The effect of cleaning and disinfecting the sampling well on the microbial communities of deep subsurface water samples

2005 ◽  
Vol 7 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Odile Basso ◽  
Jean-Francois Lascourreges ◽  
Marc Jarry ◽  
Michel Magot
Keyword(s):  
Microbial Communities ◽  
Water Samples ◽  
Subsurface Water ◽  
Deep Subsurface

scholarly journals Cultivation and Molecular Studies to Reveal the Microbial Communities of Groundwaters Discharge Located in Hungary

Water ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1533
Author(s):  
Marwene Toumi ◽  
Gorkhmaz Abbaszade ◽  
Yousra Sbaoui ◽  
Rózsa Farkas ◽  
Éva Ács ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Water Samples ◽  
Methanogenic Archaea ◽  
Amplicon Sequencing ◽  
Diversity Indices ◽  
High Ratio ◽  
Subsurface Water ◽  
Ammonia Oxidizer ◽  
Bacterial Phyla ◽  
Cell Counts

In the present study 12 water samples of five sampling sites (Tatabánya, Dandár, Szentendre, Szent Flórián and Ciprián groundwaters) known as nutrient-depleted aquatic environments were studied using amplicon sequencing (NGS) and cultivation techniques. Diversity indices and cell counts were determined to assess the species richness in relation to the cell counts within the samples, and the oligocarbophile growth capability of the isolated bacteria was tested in microtiter plates. Altogether, 55 bacterial phyla were identified from the samples by amplicon sequencing. The microbial communities of the different sampling times of the same sites did not differ significantly. Patescibacteria and Proteobacteria were present in all samples. Ciprián sample was dominated by Bacteroidetes, while in Dandár sample a high ratio of Chloroflexi was detected. Rokubacteria and WOR-1 dominated Szent Flórián sample and Tatabánya had a high number of Epsilonbacteraeota. Nine archaeal phyla were also detected; the samples were characterized by the presence of unclassified archaea and Nanoarchaeota, among them Woesearchaeia, as the most dominant. Crenarchaeota and Altiarchaeota were detected in high ratios in Dandár water samples. Among Thaumarchaeota the family Nitrosopumilaceae, and orders of Nitrosotaleales and Nitrososphaerales appeared in Szent Flórián and Tatabánya samples. Key organisms of the different biogeochemical cycles were discovered in these nutrient-depleted environments: methanogenic archaea, methanotrophic bacteria, ammonia oxidizer, nitrate reducers, diazotrophs, sulfate reducers, and sulfur oxidizer. Diversity indices and cell counts of the samples show negative correlation in case of bacteria and positive in case of archaea in Ciprián sample. The high diversity indices in Szentendre samples are connected to low cell counts, most probably due to the vulnerability of the groundwaters to the external environment factors which lead to the infiltration of soil microbes and contaminants to the water. The isolated bacteria were affiliated into four phyla, most of them belonging to Proteobacteria (59%) followed by Actinobacteria (21%), Firmicutes (17%) and Verrucomicrobia (1%). The members of the facultative chemolithotrophic genera of Sphingobium, Sphingomonas, Sphingopyxis were characterizing only Szentendre, Szent Flórián and Tatabánya samples. Only 10% of the isolated species showed an obligate oligocarbophile character. From the samples, a high number of novel bacterial taxa were cultivated. As a conclusion, our results confirmed the predominance of unclassified and unknown taxa in subsurface water, pointing to the importance and necessity of further studies to characterize these microbial populations.

scholarly journals Shaping of the Present-Day Deep Biosphere at Chicxulub by the Impact Catastrophe That Ended the Cretaceous

2021 ◽  
Vol 12 ◽  
Author(s):  
Charles S. Cockell ◽  
Bettina Schaefer ◽  
Cornelia Wuchter ◽  
Marco J. L. Coolen ◽  
Kliti Grice ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Amplicon Sequencing ◽  
Granitic Rocks ◽  
Rrna Gene ◽  
Deep Biosphere ◽  
Deep Subsurface ◽  
Cell Biomass ◽  
Colonization Potential ◽  
The Impact

We report on the effect of the end-Cretaceous impact event on the present-day deep microbial biosphere at the impact site. IODP-ICDP Expedition 364 drilled into the peak ring of the Chicxulub crater, México, allowing us to investigate the microbial communities within this structure. Increased cell biomass was found in the impact suevite, which was deposited within the first few hours of the Cenozoic, demonstrating that the impact produced a new lithological horizon that caused a long-term improvement in deep subsurface colonization potential. In the biologically impoverished granitic rocks, we observed increased cell abundances at impact-induced geological interfaces, that can be attributed to the nutritionally diverse substrates and/or elevated fluid flow. 16S rRNA gene amplicon sequencing revealed taxonomically distinct microbial communities in each crater lithology. These observations show that the impact caused geological deformation that continues to shape the deep subsurface biosphere at Chicxulub in the present day.

Procedures for Sampling Deep Subsurface Microbial Communities in Unconsolidated Sediments

1992 ◽  
Vol 12 (1) ◽  
pp. 96-104 ◽  
Author(s):  
Brent F. Russell ◽  
Tommy J. Phelps ◽  
William T. Griffin ◽  
Kenneth A. Sargent
Keyword(s):  
Microbial Communities ◽  
Unconsolidated Sediments ◽  
Deep Subsurface

scholarly journals Ion Chromatographic Determination of Sulfate Ion in Deep Subsurface Water and Subsurface Brine

2014 ◽  
Vol 63 (5) ◽  
pp. 405-409
Author(s):  
Machie OTSUKA ◽  
Takehiro SHIBUYA ◽  
Hidenori KANETA ◽  
Makoto NONOMURA
Keyword(s):  
Chromatographic Determination ◽  
Subsurface Water ◽  
Sulfate Ion ◽  
Deep Subsurface

scholarly journals Surface and subsurface water quality assessment in semi-arid region: a case study from Quetta and Sorange Intermontane Valleys, Pakistan

2014 ◽  
Vol 16 (5) ◽  
pp. 938-954 ◽  
Keyword(s):  
Water Samples ◽  
Central Valley ◽  
Water Quality Assessment ◽  
Hydrochemical Facies ◽  
Subsurface Water ◽  
Rock Types ◽  
Sodium Percent ◽  
Semi Arid Region ◽  
Siwalik Group ◽  
Quetta Valley

<div> <p>Present investigation is an exertion to explore safe means of water for the community in the water scare region. In order to accomplish such goal origin of springs as well as chemical analyses of groundwater samples from different locations were carried out. Four zones are discovered on the basis of nature of water points, rock types and water chemistry. These zones are Hard Rocks Springs (HRS), Valley Springs (VS), Recharge Areas Aquifers (RAA) and Central Valley Aquifers (CVA). HRS are originated from thick successions of limestone (Kirthar Limestone) and thick packages of channelized sandstone and conglomerates of the Siwalik Group. It is recommended that Chiltan, Parh, Kirthar, Dhok Pathan and Soan formations may be focused for future needs of safe water supply in the Quetta valley. This study includes the analysis of different physico-chemical parameters such as Total dissolved solids (TDS), pH, turbidity, Electrical conductivity (EC), hydrochemical facies, Sodium Adsorption Ration (SAR), Sodium Percent (Na%), Kelleys Index (KI), Permeability Index (PI), major cation and anions to assess the quality of groundwater sources. The outcomes of groundwater analyses were equated with various drinking water standards e.g., NSDWQ-Pak, USEPA, and EU. The results are plotted for determination of lateral variations and to empathize the reasons of these variations. The affects of rock types, geological structures as well as anthropogenic causes on origin and results are discussed systematically. The hydrochemical facies identified show that the alkaline earth exceeds alkalies, strong acids exceeds weak acids, mixed type (no cation, anion exceeds 50%), no dominant cation and anion types but water of VS is calcium chloride and sulfate type. The water is classified as C2-S1 (HRS), C3-S1 (CVA &amp; RAA) and C3-S2 (VS) based on SAR and EC values, which intend that most water samples can be used for irrigation safely except water samples collected from VS. Based on the Na% the water samples are categorized as excellent to good and good to permissible limits. The water is suitable for irrigation as all samples bear KI less than 1. The PI values in the water samples studied (less than 40) also contemplate that the water is desirable for irrigation use, although caution is needed of VS water.</p> </div> <p>&nbsp;</p>

scholarly journals Radiolytic H2in continental crust: Nuclear power for deep subsurface microbial communities

2005 ◽  
Vol 6 (7) ◽  
pp. n/a-n/a ◽  
Author(s):  
Li-Hung Lin ◽  
James Hall ◽  
Johanna Lippmann-Pipke ◽  
Julie A. Ward ◽  
Barbara Sherwood Lollar ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Continental Crust ◽  
Nuclear Power ◽  
Deep Subsurface

scholarly journals Differences in microbial community composition between injection and production water samples of water flooding petroleum reservoirs

2015 ◽  
Vol 12 (11) ◽  
pp. 3403-3414 ◽  
Author(s):  
P. K. Gao ◽  
G. Q. Li ◽  
H. M. Tian ◽  
Y. S. Wang ◽  
H. W. Sun ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Water Samples ◽  
High Throughput Sequencing ◽  
Strong Association ◽  
Microbial Community Composition ◽  
Petroleum Reservoirs ◽  
Water Flooding ◽  
Microbial Populations ◽  
Production Wells ◽  
Sandstone Reservoir

Abstract. Microbial communities in injected water are expected to have significant influence on those of reservoir strata in long-term water flooding petroleum reservoirs. To investigate the similarities and differences in microbial communities in injected water and reservoir strata, high-throughput sequencing of microbial partial 16S rRNA of the water samples collected from the wellhead and downhole of injection wells, and from production wells in a homogeneous sandstone reservoir and a heterogeneous conglomerate reservoir were performed. The results indicate that a small number of microbial populations are shared between the water samples from the injection and production wells in the sandstone reservoir, whereas a large number of microbial populations are shared in the conglomerate reservoir. The bacterial and archaeal communities in the reservoir strata have high concentrations, which are similar to those in the injected water. However, microbial population abundance exhibited large differences between the water samples from the injection and production wells. The number of shared populations reflects the influence of microbial communities in injected water on those in reservoir strata to some extent, and show strong association with the unique variation of reservoir environments.

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