The microbial community structure of different permeable sandy sediments characterized by the investigation of bacterial fatty acids and fluorescence in situ hybridization

2005 ◽  
Vol 7 (2) ◽  
pp. 281-293 ◽  
Author(s):  
S. I. Buhring ◽  
M. Elvert ◽  
U. Witte
Keyword(s):  
Fatty Acids ◽  
In Situ Hybridization ◽  
Community Structure ◽  
Microbial Community ◽  
Fluorescence In Situ Hybridization ◽  
Microbial Community Structure ◽  
Sandy Sediments

Effects of hydroxylamine on microbial community structure and function of autotrophic nitrifying biofilms determined by in situ hybridization and the use of microelectrodes

2004 ◽  
Vol 49 (11-12) ◽  
pp. 61-68 ◽  
Author(s):  
T. Kindaichi ◽  
S. Okabe ◽  
H. Satoh ◽  
Y. Watanabe
Keyword(s):  
In Situ Hybridization ◽  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Structure And Function ◽  
Ammonia Oxidizing Bacteria ◽  
Low Concentrations ◽  
Nitrite Oxidizing Bacteria ◽  
And Function

Effects of hydroxylamine (NH2OH), an intermediate of NH4+ oxidation, on microbial community structure and function of two autotrophic nitrifying biofilms fed with and without NH2OH were analyzed by a 16S rRNA approach and the use of microelectrodes. In the NH2OH-added biofilm, partial oxidation of NH4+ to NO2- was observed, whereas complete oxidation of NH4+ to NO3- was achieved in the control biofilm. In situ hybridization results revealed that no nitrite-oxidizing bacteria (NOB) hybridized with any specific probes were detected in the NH2OH-added biofilm. Thus, the addition of low concentrations of NH2OH (250 mM) completely inhibited the growth of NOB. Phylogenetic analysis of 16S rDNA indicated that the ammonia-oxidizing bacteria (AOB) detected in both biofilms were closely related to Nitrosomonas europaea, and that the clone sequences from both biofilm libraries have more than 99% similarity to each other. However, in situ hybridization results revealed that the addition of NH2OH changed the form of growth pattern of the dominant Nitrosomonas spp. from dense clusters mode to single scattered cells mode. Microelectrode measurements revealed that the average NH4+ consumption rate calculated in the NH2OH-added biofilm was two times higher than that in the control biofilm. This clearly demonstrated that the oxidation of NH4+ was stimulated by NH2OH addition.

scholarly journals Performance and Microbial Community Structure of Anaerobic Membrane Bioreactor for Lipids-Rich Kitchen Waste Slurry Treatment: Mesophilic and Thermophilic Processes

Processes ◽  
2019 ◽  
Vol 7 (12) ◽  
pp. 879 ◽  
Author(s):  
Xiaolan Xiao ◽  
Wansheng Shi ◽  
Wenquan Ruan
Keyword(s):  
Fatty Acids ◽  
Community Structure ◽  
Microbial Community ◽  
Removal Efficiency ◽  
Microbial Community Structure ◽  
Membrane Bioreactor ◽  
Cod Removal ◽  
Kitchen Waste ◽  
Anaerobic Membrane Bioreactor ◽  
Increasing Temperature

The performance and microbial community structure for treating lipids-rich kitchen waste slurry in mesophilic Anaerobic Membrane Bioreactor (m-AnMBR) and thermophilic AnMBR (t-AnMBR) were compared in this study. Higher Organic Loading Rate (OLR) of 12 kg-COD/(m3·d), better Chemical Oxygen Demand (COD) removal efficiency over 98%, stronger stability with Volatile Fatty Acids (VFAs)/alkalinity below 0.04, higher flux with 18 L/(m2·h) and lower Long Chain Fatty Acids (LCFAs) concentration of 550 mg/L were obtained in the m-AnMBR. Directly increasing temperature from 39 to 55 °C resulted in a collapse of the t-AnMBR. Acclimation via gradually increasing temperature made the t-AnMBR run successfully with lower OLR and COD removal efficiency of 7.5 kg-COD/(m3·d) and 96%. An obvious discrepancy of microbial community structure was presented between the m-AnMBR and t-AnMBR via the 16S rRNA gene sequence analysis. The Methanomethylovorans and Methanoculleus were dominant in the t-AnMBR instead of Methanobacterium and Methanothrix in the m-AnMBR.

Sign in / Sign up

Export Citation Format

Share Document