Improved heterologous protein expression in the chloroplast of Chlamydomonas reinhardtii through promoter and 5′ untranslated region optimization

2011 ◽  
Vol 9 (6) ◽  
pp. 674-683 ◽  
Author(s):  
Beth A. Rasala ◽  
Machiko Muto ◽  
Joseph Sullivan ◽  
Stephen P. Mayfield
Keyword(s):  
Protein Expression ◽  
Chlamydomonas Reinhardtii ◽  
Untranslated Region ◽  
Heterologous Protein ◽  
Heterologous Protein Expression

scholarly journals Heterologous reporter expression in the planarian Schmidtea mediterranea through somatic mRNA transfection

2021 ◽  
Author(s):  
Richard Nelson Hall ◽  
Uri Weill ◽  
Margarita Khariton ◽  
Sergio Leal-Ortiz ◽  
Leonard Drees ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Expression ◽  
Untranslated Region ◽  
Adult Stem Cells ◽  
Heterologous Protein ◽  
Heterologous Protein Expression ◽  
Reporter Expression ◽  
Mrna Transfection ◽  
Genetic Constructs ◽  
Exogenous Gene

Planarians have long been studied for their regenerative abilities, but they possess limited genetic tools due to challenges in gene delivery, expression, and detection, despite decades of work. We developed a toolbox for heterologous protein expression in planarian cells and in live animals. Specifically, we identified and optimized nanotechnological and chemical transfection methods to efficiently deliver mRNA encoding nanoluciferase into somatic cells, including planarian adult stem cells (neoblasts). The use of a luminescent reporter allowed us to quantitatively measure protein expression through spectroscopy and microscopy, thus overcoming the strong autofluorescent background of planarian tissues. Using this platform, we investigated the use of endogenous untranslated region (UTR) sequences and codon usage bias to post-transcriptionally alter gene expression. Our work provides a strong foundation for advancing exogenous gene expression and for the rapid prototyping of genetic constructs to accelerate the development of transgenic techniques in planarians.

A chimeric hydrolase-PTXD transgene enables chloroplast-based heterologous protein expression and non-sterile cultivation of Chlamydomonas reinhardtii

2021 ◽  
Vol 59 ◽  
pp. 102429
Author(s):  
Edoardo Cutolo ◽  
Matteo Tosoni ◽  
Simone Barera ◽  
Luis Herrera-Estrella ◽  
Luca Dall'Osto ◽  
...  
Keyword(s):  
Protein Expression ◽  
Chlamydomonas Reinhardtii ◽  
Heterologous Protein ◽  
Heterologous Protein Expression

Transport proteins PotD and Crr of Escherichia coli, novel fusion partners for heterologous protein expression

2007 ◽  
Vol 1774 (12) ◽  
pp. 1536-1543 ◽  
Author(s):  
Kyung-Yeon Han ◽  
Hyuk-Seong Seo ◽  
Jong-Am Song ◽  
Keum-Young Ahn ◽  
Jin-Seung Park ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein Expression ◽  
Heterologous Protein ◽  
Transport Proteins ◽  
Heterologous Protein Expression

scholarly journals Heterologous protein expression in E. coli v3 (protocols.io.9vgh63w)

protocols.io ◽  
2019 ◽  
Author(s):  
Diep R ◽  
Timothy Rhodes ◽  
Nay Chi ◽  
Estee E ◽  
Kai Xun
Keyword(s):  
Protein Expression ◽  
Heterologous Protein ◽  
Heterologous Protein Expression ◽  
E Coli

scholarly journals Heterologous protein expression in E. coli v5 (protocols.io.bdjti4nn)

protocols.io ◽  
2020 ◽  
Author(s):  
Diep Ganguly ◽  
Timothy Rhodes ◽  
Nay Chi ◽  
Estee E ◽  
Kai Xun
Keyword(s):  
Protein Expression ◽  
Heterologous Protein ◽  
Heterologous Protein Expression ◽  
E Coli
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