scholarly journals Rickettsia spp. infection in Rhipicephalus sanguineus ticks in a Brazilian spotted fever endemic rural area in Rio de Janeiro state, Brazil

2009 ◽  
Vol 15 ◽  
pp. 245-246 ◽  
Author(s):  
T. Rozental ◽  
A.R.M. Favacho ◽  
J.D. Barreira ◽  
R.C. Oliveira ◽  
R. Gomes ◽  
...  
Keyword(s):  
Rural Area ◽  
Rio De Janeiro ◽  
Spotted Fever ◽  
Rhipicephalus Sanguineus ◽  
Brazilian Spotted Fever ◽  
Janeiro State

scholarly journals First identification of natural infection of Rickettsia rickettsii in the Rhipicephalus sanguineus tick, in the State of Rio de Janeiro

2009 ◽  
Vol 29 (2) ◽  
pp. 105-108 ◽  
Author(s):  
Nathalie C. Cunha ◽  
Adivaldo H. Fonseca ◽  
Jania Rezende ◽  
Tatiana Rozental ◽  
Alexsandra R.M. Favacho ◽  
...  
Keyword(s):  
Rio De Janeiro ◽  
Natural Infection ◽  
Spotted Fever ◽  
Pcr Amplification ◽  
Rhipicephalus Sanguineus ◽  
Rickettsia Rickettsii ◽  
Protein Encoding ◽  
Pcr Products ◽  
Brazilian Spotted Fever

The Brazilian Spotted Fever (BSF) is a zoonotic disease caused by Rickettsia rickettsii and transmitted by ticks of the genus Amblyomma, more frequently, Amblyomma cajennense. The aim of this paper was to report the first molecular detection of R. rickettsii on R. sanguineus naturally infected in Rio de Janeiro, Brazil. Ticks were collected from dogs in a rural region of Resende municipality, Rio de Janeiro State, Brazil (22º30'9.46"S, 44º42'44.29"WO), where occurred five human cases of BSF in 2006. The ticks were identified under a stereoscopic microscope and separated in pools by stages, species and sex. DNA extraction was carried out using QIAamp DNA Mini Kit (QIAGEN®). The DNA was submitted to PCR amplification using 04 set of primers: Rr190.70p/Rr190.602n (OmpA, 532bp), BG1-21/BG2-20 (OmpB, 650bp), Tz15/Tz16 (17 kDa protein-encoding gene, 246bp) and RpCS.877p/RpCS.1258n (gltA, 381bp). PCR products were separated by electrophoresis on 1% agarose gels and visualized under ultraviolet light with ethidium bromide. PCR products of the expected sizes were purified by QIAquick® and sequenced by ABI PRISM®. The generated nucleotide sequences were edited with using Bioedit® software and compared with the corresponding homologous sequences available through GenBank, using Discontiguous Mega Blast (http://www.ncbi.nlm.nih.gov). It was confirmed R. rickettsii by sequencing of the material (GenBank FJ356230). The molecular characterization of R. rickettsii in the tick R. sanguineus emphasizes the role of dogs as carriers of ticks from the environment to home. Moreover, this result suggests that there is a considerable chance for active participation of R. sanguineus as one of tick species in the transmission of R. ricketsii to human being in the Brazilian territory.

scholarly journals Seroprevalence of Rickettsia rickettsii and Rickettsia parkeri in dogs during a Brazilian Spotted Fever outbreak in the State of Rio de Janeiro

2018 ◽  
Vol 70 (3) ◽  
pp. 667-674 ◽  
Author(s):  
I.T. Poubel ◽  
N.C. Cunha ◽  
A.B.M. Fonseca ◽  
A. Pinter ◽  
A.H. Fonseca ◽  
...  
Keyword(s):  
Rio De Janeiro ◽  
Spotted Fever ◽  
Immunofluorescence Assay ◽  
Spotted Fever Group ◽  
Ambient Conditions ◽  
Rickettsia Parkeri ◽  
Rickettsia Rickettsii ◽  
Brazilian Spotted Fever ◽  
Spotted Fever Group Rickettsia ◽  
Janeiro State

ABSTRACT The present paper is the first to perform this evaluation in dogs from the cities of Natividade, Porciuncula and Varre-Sai. The aim of this study is to search for Spotted Fever Group Rickettsia in canine sera using indirect immunofluorescence assay and to identify the probable causative agent of sera reactions in animals. Of the 253 sampled canines, 67.59% (171/253) were seroreactive for Rickettsia rickettsii and 11.07% (28/253) for Rickettsia parkeri, both in dilution 1:64. Titration of tested sera against R. rickettsii antigens reached 1:131.072 and, for R. parkeri, 1:4.096. We conclude that dogs are important sentinels for R. rickettsii infection, and can be infected regardless of sex, age, the habit of visiting woodlands or being in direct contact with equines and capybaras. Serological diagnosis has highlighted many dogs infected by R. rickettsii, and ambient conditions, such as the presence of flowing water bodies, was important for the occurrence of Brazilian Spotted Fever in the northwestern of Rio de Janeiro State.

scholarly journals Intelligent monitoring of Aedes aegypti in a rural area of Rio de Janeiro State, Brazil

2017 ◽  
Vol 59 (0) ◽  
Author(s):  
Argemiro Sanavria ◽  
Claudia Bezerra da Silva ◽  
Érica Heleno Electo ◽  
Lidiane Cristina Rocha Nogueira ◽  
Sandra Maria Gomes Thomé ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Rural Area ◽  
Rio De Janeiro ◽  
Intelligent Monitoring ◽  
Janeiro State

scholarly journals Serosurvey of Rickettsia spp. in cats from a Brazilian spotted fever-endemic area

2019 ◽  
Vol 28 (4) ◽  
pp. 713-721
Author(s):  
Juliana Cristina Rebonato Mendes ◽  
Louise Bach Kmetiuk ◽  
Camila Marinelli Martins ◽  
Aurea Maria Oliveira Canavessi ◽  
Tatiana Jimenez ◽  
...  
Keyword(s):  
Endemic Area ◽  
Spotted Fever ◽  
Rhipicephalus Sanguineus ◽  
Southeastern Brazil ◽  
Rickettsia Parkeri ◽  
Rickettsia Felis ◽  
Rickettsia Species ◽  
Brazilian Spotted Fever ◽  
Endemic Areas ◽  
The University

Abstract Rickettsia spp. bacteria are responsible for tick-borne diseases worldwide, mostly maintained by rickettsial amplifiers capybaras in Brazilian endemic areas. The campus of the University of São Paulo, in southeastern Brazil, is an area endemic for Brazilian spotted fever (BSF), with high density of capybaras and Amblyomma spp., along with confirmed human cases. Besides capybaras, the university has also an in-campus high population of sheltered and free-roaming cats. Accordingly, the aim of this study was to determine the prevalence and characteristics associated with Rickettsia rickettsii, Rickettsia parkeri and Rickettsia felis exposure among cats in a BSF-endemic area. Out of 51 cats sampled, 23/35 shelter (65.7%) and 5/16 free-roaming (31.2%) were positive (titers ≥ 64) for at least one Rickettsia species. Ticks species were present in 3/16 free-roaming cats (18.8%), consisting of Amblyomma spp., nymphs of Amblyomma sculptum and adult Rhipicephalus sanguineus sensu lato. Despite sharing the capybaras environment, the seropositivity among the free-roaming and shelter cats was lower than owned cats in other endemic areas. Whether equally or less exposed to rickettsial infection, compared with owned cats in endemic areas, free-roaming and shelter cats may be used as environmental sentinels for human exposure to rickettsiae in such areas.

scholarly journals Atypical lymphocytosis in leptospirosis: a cohort of hospitalized cases between 1996 and 2009 in State of Rio de Janeiro, Brazil

2011 ◽  
Vol 44 (5) ◽  
pp. 611-615 ◽  
Author(s):  
Paulo Vieira Damasco ◽  
Carlos André Lins Ávila ◽  
Angélica Tápia Barbosa ◽  
Marilza de Moura Ribeiro Carvalho ◽  
Geraldo Moura Batista Pereira ◽  
...  
Keyword(s):  
Rio De Janeiro ◽  
Spotted Fever ◽  
Health Concern ◽  
Disease Course ◽  
Public Health Concern ◽  
Partial Protection ◽  
Cell Responses ◽  
Diagnostic Confusion ◽  
Brazilian Spotted Fever ◽  
Overall Mortality

INTRODUCTION: Leptospirosis is a zoonotic disease found in tropical and temperate countries, and its clinical diagnostic confusion with arboviruses (dengue fever, oropouche fever and yellow fever), Brazilian spotted fever, viral hepatitis and hantaviruses has been an ongoing public health concern. The aim of this observational study was to demonstrate an association between findings of atypical lymphocytosis and the progression of endemic leptospirosis. METHODS: A retrospective analysis was performed on the demographic, epidemiological, clinical and laboratory aspects of 27 human leptospirosis cases that occurred over a period of 13 years (1996-2009) with no reported epidemic outbreaks in Rio de Janeiro, Brazil. RESULTS: The overall mortality rate was 11.1% in our cohort of hospitalized cases. However, there was no mortality among patients with atypical lymphocytosis (OR = 11.1; 95% CI = 1.12-110.9; p = 0.04). Two patients who were in the septicemic phase showed signs of expansion of γδ T cell responses in peripheral blood. CONCLUSIONS: Atypical lymphocytosis may be observed in patients with leptospirosis. Our observations suggest that these atypical leukocyte subsets are associated with partial protection during the disease course of leptospirosis.

scholarly journals Epidemiological aspects of the Brazilian spotted fever: seasonal activity of ticks collected in an endemic area in São Paulo, Brazil

1997 ◽  
Vol 30 (3) ◽  
pp. 181-185 ◽  
Author(s):  
Elba R.S. de Lemos ◽  
Raimundo D. Machado ◽  
José R. Coura ◽  
Maria A.A. Guimarães ◽  
Nicolau M. Serra Freire ◽  
...  
Keyword(s):  
Endemic Area ◽  
Spotted Fever ◽  
Rhipicephalus Sanguineus ◽  
Sao Paulo ◽  
Boophilus Microplus ◽  
Seasonal Activity ◽  
São Paulo ◽  
Amblyomma Triste ◽  
Brazilian Spotted Fever ◽  
One Year

Ticks were collected from vegetation and animals at monthly intervals during one year (1993-1994) in an endemic area of Brazilian spotted fever in the County of Pedreira, State of São Paulo. Six species of ticks were identified Amblyomma cajennense, Amblyomma cooperi, Amblyomma triste, Anocentor nitens, Rhipicephalus sanguineus and Boophilus microplus. Only the first species was sufficiently numerous to permit a quantitative study with seasonal activity, although the distribution and source of capture of other species were observed and are reported. This information is correlated with the epidemiology of tick-borne rickettsiosis.

scholarly journals Characterization of rickettsia rickettsii in a case of Fatal Brazilian spotted fever in the city of Rio de Janeiro, Brazil

2008 ◽  
Vol 12 (2) ◽  
Author(s):  
Cristiane Lamas ◽  
Alexsandra Favacho ◽  
Tatiana Rozental ◽  
Márcio N. Bóia ◽  
Andrei H. Kirsten ◽  
...  
Keyword(s):  
Rio De Janeiro ◽  
Spotted Fever ◽  
Rickettsia Rickettsii ◽  
Brazilian Spotted Fever ◽  
The City

scholarly journals Rickettsial pathogens circulating in urban districts of Rio de Janeiro, without report of human Brazilian Spotted Fever

2020 ◽  
Vol 29 (4) ◽  
Author(s):  
Sabrina Destri Emmerick Campos ◽  
Nathalie Costa da Cunha ◽  
Camila de Souza Cerqueira Machado ◽  
Erich Loza Telleria ◽  
Matheus Dias Cordeiro ◽  
...  
Keyword(s):  
Rural Areas ◽  
Urban Areas ◽  
Rio De Janeiro ◽  
Spotted Fever ◽  
Rhipicephalus Sanguineus ◽  
Emerging Diseases ◽  
Immunofluorescence Assay ◽  
Spotted Fever Group ◽  
Indoor Environments ◽  
Rhipicephalus Sanguineus Sensu Lato

Abstract Spotted fever group rickettsioses are emerging diseases. In some of these diseases, domestic dogs act as sentinels. Canine serological studies have demonstrated that rickettsial dispersion is concentrated in rural areas, seroprevalence being higher where human rickettsioses are endemic. In Rio de Janeiro, the Atlantic forest vegetation has been devastated by urbanization. In this context, we aimed to detect Rickettsia spp. in urban areas of the West Zone of Rio de Janeiro. Sera from 130 dogs were tested by Indirect Immunofluorescence Assay, and ticks collected from these dogs were tested by polymerase chain reaction. We found the rate of serological reactions against R. rickettsii and R. parkeri in our study area to exceed those of rural and non-endemic areas, highlighting the importance of dogs as urban sentinels. The possibility of contact with opossums and capybaras increased the chances of exposure to Rickettsia spp., reinforcing the hypothetical link between the landscape and the rickettsial wild cycle. Rhipicephalus sanguineus sensu lato was the tick most frequently observed. PCR-positive samples showed similarity with R. rickettsii and R. felis, an emerging pathogen rarely reported from ticks. We observed that rickettsiae circulate in urban places and ticks from indoor environments, which may be involved in bacterial epidemiology.

scholarly journals Evidence of spotted fever group rickettsiae in state of Rio de Janeiro, Brazil

2002 ◽  
Vol 44 (3) ◽  
pp. 155-158 ◽  
Author(s):  
Tatiana ROZENTAL ◽  
Maria Cristina BUSTAMANTE ◽  
Marinete AMORIM ◽  
Nicolau Maués SERRA-FREIRE ◽  
Elba Regina Sampaio de LEMOS
Keyword(s):  
Rio De Janeiro ◽  
Spotted Fever ◽  
Rhipicephalus Sanguineus ◽  
Direct Immunofluorescence ◽  
Spotted Fever Group ◽  
Limited Information ◽  
Amblyomma Cajennense ◽  
Preliminary Results ◽  
Spotted Fever Group Rickettsiae ◽  
Spotted Fever Group Rickettsia

Ticks were obtained from dogs from February to September of 1999 at weekly intervals, in the County of Piraí, State of Rio de Janeiro. Four hundred seventy four ixodids were taxonomically identified, 103 Amblyomma cajennense, seven Amblyomma ovale, 209 Rhipicephalus sanguineus, and 155 Amblyomma sp. An hemolymph test associated with Giemsa's stain revealed two specimens in 163 ticks tested (R. sanguineus and Amblyomma sp), containing rickettsia-like organisms. Direct immunofluorescence verified the presence of spotted fever group rickettsia in one specimen of R. sanguineus. Considering the limited information on rickettsiosis in Brazil, principally in relation to the vectors involved in perpetuating it in foci, these preliminary results give us an idea on the importance of infection in ticks, allowing to expand our knowledge on this zoonosis.

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