Induction of Apoptosis and Necrosis in Human Peripheral Blood Mononuclear Cells by Fatty Acid Ethyl Esters

2008 ◽  
Vol 32 (3) ◽  
pp. 534-543 ◽  
Author(s):  
Khaled Alhomsi ◽  
Martin Selig ◽  
Tonci Sustic ◽  
Eyad Katrangi ◽  
Volkmar Weissig ◽  
...  
2007 ◽  
Vol 99 (1) ◽  
pp. 147-154 ◽  
Author(s):  
Camilla T. Damsgaard ◽  
Hanne Frøkiær ◽  
Lotte Lauritzen

Dietary intake of 18: 2n-6 and 18: 3n-3 may affect endogenous production and incorporation of n-3 long-chain PUFA (LCPUFA) from fish oils (FO). This double-blinded controlled 2 × 2-factorial 8-week intervention investigates the effects of high and low 18: 2n-6 intake in combination with FO-supplementation on tissue fatty acid composition. Healthy young men (n 64) were randomized to capsules with FO or olive oil (control) (4·4 (2·0–5·6) ml/d) and to either sunflower oil and margarine (S/B) or rapeseed oil and a butter spread (R/K) to provide a high or a low 18: 2n-6 intake. Diet was measured by 4-d weighed dietary records at baseline, during and 8 weeks after the intervention and tissue incorporation as fatty acid composition of peripheral blood mononuclear cells (PBMC). The fat intervention gave a mean difference in the 18: 2n-6 intake of 7·3 g/d (95 % CI 4·6, 10·0) and a similar 18: 3n-3 intake in the groups. The R/K groups had a 0·2 % fatty acid (FA%) (95 % CI 0·0, 0·4, P = 0·02) higher content of 22: 5n-3 in the PBMC, a tendency of slightly higher 20: 5n-3 (P = 0·06), but no more 22: 6n-3 (P = 0·83) than the S/B groups. FO effectively raised the PBMC content of all n-3 LCPUFA (P < 0·001). The fat intervention did not markedly influence the effect of FO; the mean PBMC content of n-3 LCPUFA was 10·3 (sem 0·3) FA% in the FO+S/B group and 10·6 (sem 0·2) FA% in the FO+R/K group. In conclusion, increasing the 18: 2n-6 intake did not have any pronounced effect on incorporation of n-3 LCPUFA in PBMC, either alone or with simultaneous FO supplementation.


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