Nucleotide Sequences of DNA Fragments of Encephalitozoon cuniculi Amplified by Polymerase Chain Reaction with Primers Regarded as Specific for Echinococcus

1996 ◽  
Vol 43 (3) ◽  
pp. 217-221 ◽  
Author(s):  
HIDEKI NAGANO ◽  
CHIAKI SATOH ◽  
KOJI FURUYA
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleotide Sequences ◽  
Dna Fragments ◽  
Encephalitozoon Cuniculi ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Encephalitozoon Cuniculi Placentitis and Abortion in a Quarterhorse Mare

2003 ◽  
Vol 15 (1) ◽  
pp. 57-59 ◽  
Author(s):  
J. C. Patterson-Kane ◽  
P. Caplazi ◽  
F. Rurangirwa ◽  
R. R. Tramontin ◽  
K. Wolfsdorf
Keyword(s):  
Electron Microscopy ◽  
Polymerase Chain Reaction ◽  
Epithelial Cells ◽  
Ribosomal Dna ◽  
Encephalitozoon Cuniculi ◽  
Chain Reaction ◽  
University Of Kentucky ◽  
Livestock Disease ◽  
Diagnostic Center ◽  
Polymerase Chain

Encephalitozoon cuniculi is a microsporidial parasite, which has rarely been reported to cause placentitis in animals. A late-term aborted fetus and placenta from a Quarterhorse were presented to the Livestock Disease Diagnostic Center, University of Kentucky, for diagnostic examination. There was a necrotizing placentitis, with distension of many chorionic epithelial cells by intracytoplasmic vacuoles containing 1–2-μm-diameter, elongated, gram-positive organisms. The organisms were identified as E. cuniculi by electron microscopy and by polymerase chain reaction using primers to microsporidial ribosomal DNA. Joints of the fetus were swollen, with gross and microscopic lesions of synovitis; however, E. cuniculi DNA was not detected.

Plasma vs. serum in circulating tumor DNA measurement: characterization by DNA fragment sizing and digital droplet polymerase chain reaction

2020 ◽  
Vol 58 (4) ◽  
pp. 527-532 ◽  
Author(s):  
Jee-Soo Lee ◽  
Miyoung Kim ◽  
Moon-Woo Seong ◽  
Han-Sung Kim ◽  
Young Kyung Lee ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Circulating Tumor Dna ◽  
Analytical Sensitivity ◽  
Dna Fragments ◽  
Chain Reaction ◽  
Specimen Type ◽  
Dna Fragment ◽  
Polymerase Chain ◽  
Ctdna Analysis ◽  
Tumor Dna

AbstractBackgroundChoosing the specimen type is the first step of the pre-analytical process. Previous reports suggested plasma as the optimal specimen for circulating tumor DNA (ctDNA) analysis. However, head-to-head comparisons between plasma and serum using platforms with high analytical sensitivity, such as droplet digital polymerase chain reaction (ddPCR), are limited, and several recent studies have supported the clinical utility of serum-derived ctDNA. This study aimed to compare the DNA profiles isolated from plasma and serum, characterize the effects of the differences between specimens on ctDNA measurement, and determine the major contributors to these differences.MethodsWe isolated cell-free DNA (cfDNA) from 119 matched plasma/serum samples from cancer patients and analyzed the cfDNA profiles by DNA fragment sizing. We then assessed KRAS mutations in ctDNA from matched plasma/serum using ddPCR.ResultsThe amount of large DNA fragments was increased in serum, whereas that of cfDNA fragments (<800 bp) was similar in both specimens. ctDNA was less frequently detected in serum, and the KRAS-mutated fraction in serum was significantly lower than that in plasma. The differences in ctDNA fractions between the two specimen types correlated well with the amount of large DNA fragments and white blood cell and neutrophil counts.ConclusionsOur results provided detailed insights into the differences between plasma and serum using DNA fragment sizing and ddPCR, potentially contributing to ctDNA analysis standardization. Our study also suggested that using plasma minimizes the dilution of tumor-derived DNA and optimizes the sensitivity of ctDNA analysis. So, plasma should be the preferred specimen type.

Direct Sequencing of DNA Fragments Amplified with Single Primers by Polymerase Chain Reaction without Cloning

1996 ◽  
Vol 241 (1) ◽  
pp. 136-139 ◽  
Author(s):  
Masayoshi Iizuka ◽  
Yuki Sugiyama ◽  
Shigeru Iida ◽  
Takao Sekiya
Keyword(s):  
Polymerase Chain Reaction ◽  
Direct Sequencing ◽  
Dna Fragments ◽  
Chain Reaction ◽  
Polymerase Chain

Identification of concealed disturbance of blood coagulation (deficiency of factor XI) by polymerase chain reaction (experimental study)

2015 ◽  
Vol 6 (3) ◽  
pp. 69-73
Author(s):  
Yessengali Serikovich Ussenbekov ◽  
Orik Orazimanovna Zhanserkenova ◽  
Shinar Nikolaevna Kasymbekova ◽  
Sarsenbek Torekhanovich Siyabekov ◽  
Ivan Viktorovich Sobolev ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Clotting ◽  
Autosomal Recessive ◽  
Coagulation Factor ◽  
Nucleotide Sequences ◽  
Holstein Cows ◽  
Clotting Factor ◽  
Chain Reaction ◽  
Factor Xi ◽  
Polymerase Chain

Minor bleeding is quite common in children and in some cases masks the serious disease of blood clotting. As a rule, this rare inherited disease associated with deficiency of coagulation factors as the I, II, V, VII, X, XI and XIII, as well as deficiency conjugate, most often, the joint failure factors V and VIII and factor whose synthesis associated with vitamin K. The pediatric clinic is difficult to fulfill a randomized trial because of the difficulty of identifying such children carriers of genetic abnormalities at a specific blood clotting factor. In connection with the model of deficiency of coagulation factor XI in a mammals (Bos Taurus L) with autosomal recessive type of inheritance is particularly promising. Deficiency of coagulation factor XI in cattle is inherited autosomal recessive defect. At the first time this pathology was recognized in Holstein cows in 1969. Frequently the etiologic factor of most hidden genetic defects in animals are point mutations in the coding region of the respective genes. On the contrary it has been found that deficiency of coagulation factor XI cattle (FXID) is a consequence of the insertion of nucleotide sequences within exon 12 of the gene FXI length of 76 base pairs. STOP codon (TAA) was resulted from insertion. Phenotypically deficiency of factor XI (FXID) in calves is resulted in disturbance of blood clotting and characterized by prolonged bleeding from the umbilical cord and anemia. Cows which are heterozygous in deficiency of coagulation factor XI have colostrum pink color. Those animals are frequently suffered from pneumonia, mastitis and endometritis. We monitored the breeding sires and Holstein cows on the carrier of the genetic disease: deficiency of coagulation factor XI. To detect the insertion of nucleotide sequences of 76 bp in size it is recommended to use the polymerase chain reaction.

scholarly journals In utero transmission of Encephalitozoon cuniculi strain type I in rabbits

2003 ◽  
Vol 37 (2) ◽  
pp. 132-138 ◽  
Author(s):  
P. J. R. Baneux ◽  
F. Pognan
Keyword(s):  
Polymerase Chain Reaction ◽  
In Utero ◽  
Type I ◽  
Transplacental Transmission ◽  
Encephalitozoon Cuniculi ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Strain Type ◽  
Polymerase Chain

Pregnant rabbits were serologically diagnosed as having been infected with Encephalitozoon cuniculi. At necropsy at 28 days of gestation, does, placentas and fetuses were found to be infected with E. cuniculi strain type I as evidenced by using the nested-polymerase chain reaction (PCR) technique, thereby confirming vertical transplacental transmission.

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