Polymerase Chain Reaction and Culture Confirmation of Disseminated Encephalitozoon cuniculi in a Patient with AIDS: Successful Therapy with Albendazole

1995 ◽  
Vol 171 (5) ◽  
pp. 1375-1378 ◽  
Author(s):  
M. A. De Groote ◽  
G. Visvesvara ◽  
M. L. Wilson ◽  
N. J. Pieniazek ◽  
S. B. Slemenda ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Successful Therapy ◽  
Encephalitozoon Cuniculi ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Encephalitozoon Cuniculi Placentitis and Abortion in a Quarterhorse Mare

2003 ◽  
Vol 15 (1) ◽  
pp. 57-59 ◽  
Author(s):  
J. C. Patterson-Kane ◽  
P. Caplazi ◽  
F. Rurangirwa ◽  
R. R. Tramontin ◽  
K. Wolfsdorf
Keyword(s):  
Electron Microscopy ◽  
Polymerase Chain Reaction ◽  
Epithelial Cells ◽  
Ribosomal Dna ◽  
Encephalitozoon Cuniculi ◽  
Chain Reaction ◽  
University Of Kentucky ◽  
Livestock Disease ◽  
Diagnostic Center ◽  
Polymerase Chain

Encephalitozoon cuniculi is a microsporidial parasite, which has rarely been reported to cause placentitis in animals. A late-term aborted fetus and placenta from a Quarterhorse were presented to the Livestock Disease Diagnostic Center, University of Kentucky, for diagnostic examination. There was a necrotizing placentitis, with distension of many chorionic epithelial cells by intracytoplasmic vacuoles containing 1–2-μm-diameter, elongated, gram-positive organisms. The organisms were identified as E. cuniculi by electron microscopy and by polymerase chain reaction using primers to microsporidial ribosomal DNA. Joints of the fetus were swollen, with gross and microscopic lesions of synovitis; however, E. cuniculi DNA was not detected.

scholarly journals In utero transmission of Encephalitozoon cuniculi strain type I in rabbits

2003 ◽  
Vol 37 (2) ◽  
pp. 132-138 ◽  
Author(s):  
P. J. R. Baneux ◽  
F. Pognan
Keyword(s):  
Polymerase Chain Reaction ◽  
In Utero ◽  
Type I ◽  
Transplacental Transmission ◽  
Encephalitozoon Cuniculi ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Strain Type ◽  
Polymerase Chain

Pregnant rabbits were serologically diagnosed as having been infected with Encephalitozoon cuniculi. At necropsy at 28 days of gestation, does, placentas and fetuses were found to be infected with E. cuniculi strain type I as evidenced by using the nested-polymerase chain reaction (PCR) technique, thereby confirming vertical transplacental transmission.

Incidence of hepatitis B viraemia, detected using the polymerase chain reaction, after successful therapy of hepatitis B virus carriers with interferon–α

1991 ◽  
Vol 34 (2) ◽  
pp. 114-118 ◽  
Author(s):  
William F. Carman ◽  
Spyros Dourakis ◽  
Peter Karayiannis ◽  
Mary Crossey ◽  
Rita Drobner ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Interferon Α ◽  
Successful Therapy ◽  
Chain Reaction ◽  
B Virus ◽  
Polymerase Chain

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

Detection of the Glanzmann's Thrombasthenia Mutations in Arab and Iraqi-Jewish Patients by Polymerase Chain Reaction and Restriction Analysis of Blood or Urine Samples

1991 ◽  
Vol 66 (04) ◽  
pp. 500-504 ◽  
Author(s):  
H Peretz ◽  
U Seligsohn ◽  
E Zwang ◽  
B S Coller ◽  
P J Newman
Keyword(s):  
Polymerase Chain Reaction ◽  
Base Pair ◽  
Restriction Analysis ◽  
Urine Samples ◽  
Chain Reaction ◽  
Base Pairs ◽  
Glanzmann’S Thrombasthenia ◽  
Glanzmann's Thrombasthenia ◽  
Base Pair Deletion ◽  
Polymerase Chain

SummarySevere Glanzmann's thrombasthenia is relatively frequent in Iraqi-Jews and Arabs residing in Israel. We have recently described the mutations responsible for the disease in Iraqi-Jews – an 11 base pair deletion in exon 12 of the glycoprotein IIIa gene, and in Arabs – a 13 base pair deletion at the AG acceptor splice site of exon 4 on the glycoprotein IIb gene. In this communication we show that the Iraqi-Jewish mutation can be identified directly by polymerase chain reaction and gel electrophoresis. With specially designed oligonucleotide primers encompassing the mutation site, an 80 base pair segment amplified in healthy controls was clearly distinguished from the 69 base pair segment produced in patients. Patients from 11 unrelated Iraqi-Jewish families had the same mutation. The Arab mutation was identified by first amplifying a DNA segment consisting of 312 base pairs in controls and of 299 base pairs in patients, and then digestion by a restriction enzyme Stu-1, which recognizes a site that is absent in the mutant gene. In controls the 312 bp segment was digested into 235 and 77 bp fragments, while in patients there was no change in the size of the amplified 299 bp segment. The mutation was found in patients from 3 out of 5 unrelated Arab families. Both Iraqi-Jewish and Arab mutations were detectable in DNA extracted from blood and urine samples. The described simple methods of identifying the mutations should be useful for detection of the numerous potential carriers among the affected kindreds and for prenatal diagnosis using DNA extracted from chorionic villi samples.

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