scholarly journals Isolation and characterization of a metronidazole-resistant (Mtn-R) mutant strain ofNostoc muscorumaffected in ammonium regulation of heterocyst formation and uptake hydrogenase activity

1988 ◽  
Vol 49 (2) ◽  
pp. 261-265 ◽  
Author(s):  
A.P. Kumar ◽  
D.T. Singh ◽  
H.N. Singh
Keyword(s):  
Mutant Strain ◽  
Hydrogenase Activity ◽  
Uptake Hydrogenase ◽  
Isolation And Characterization ◽  
Ammonium Regulation

scholarly journals Genetic analysis of developmental mechanisms in Hydra. II. Isolation and characterization of an interstitial cell-deficient strain

1978 ◽  
Vol 29 (1) ◽  
pp. 35-52 ◽  
Author(s):  
T. Sugiyama ◽  
T. Fujisawa
Keyword(s):  
Genetic Analysis ◽  
Mutant Strain ◽  
Interstitial Cell ◽  
Interstitial Cells ◽  
Nerve Cells ◽  
Isolation And Characterization ◽  
Developmental Mechanisms ◽  
Hydra Magnipapillata

A mutant strain (nf-I) of Hydra magnipapillata was isolated that contained no interstitial cells, nerve cells or nematocytes. This strain appeared spontaneously in a sexually inbred clone of hydra, and it was recognized by its inability to eat. When force-fed, however, it grew, multiplied by budding and regenerated. In this and in many other respects, nf-I was very similar to the interstitial cell-deficient strain produced by Campbell (1976) by means of colchicine. A chimera strain was produced by the reintroduction of interstitial cells from another strain into nf-I. The properties of nf-I, the chimera and other related strains were examined, and the possible roles that the interstitial cells and the nerve cells play in growth and morphogenesis of hydra are discussed.

scholarly journals Efficient Isolation and Characterization of a Cellulase Hyperproducing Mutant Strain of Trichoderma reesei

2018 ◽  
Vol 28 (9) ◽  
pp. 1473-1481 ◽  
Author(s):  
Zongsheng Zou ◽  
Yunying Zhao ◽  
Tingzhou Zhang ◽  
Jiaxing Xu ◽  
Aiyong He ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Mutant Strain ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of the Small Subunit of the Uptake Hydrogenase from the CyanobacteriumNostoc punctiforme

2013 ◽  
Vol 288 (25) ◽  
pp. 18345-18352 ◽  
Author(s):  
Patrícia Raleiras ◽  
Petra Kellers ◽  
Peter Lindblad ◽  
Stenbjörn Styring ◽  
Ann Magnuson
Keyword(s):  
Small Subunit ◽  
Uptake Hydrogenase ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of a high iturin yielding Bacillus velezensis UV mutant with improved antifungal activity

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0234177
Author(s):  
Young Tae Kim ◽  
Sung Eun Kim ◽  
Won Jung Lee ◽  
Zhao Fumei ◽  
Min Sub Cho ◽  
...  
Keyword(s):  
Biological Control ◽  
Antifungal Activity ◽  
Mutant Strain ◽  
Fusarium Wilt ◽  
Potential Candidate ◽  
Wild Type ◽  
Bacillus Velezensis ◽  
Sclerotinia Rot ◽  
Isolation And Characterization

To isolate Bacillus velezensis mutants with improved antifungal activity for use in the biological control of phytopathogenic fungi, wild-type Bacillus velezensis KRF-001 producing iturin, surfactin, and fengycin was irradiated by ultraviolet (UV) rays. The in vitro and in vivo antifungal activities of UV mutants and characterization of the cyclic lipopeptides produced by a selected mutant were examined. A mutant strain yielding high levels of iturin showed over 2-fold higher antifungal activity than the wild-type against Fusarium oxysporum. A potent suppressive effect of the mutant was also observed on spore germination of Botrytis cinerea, the causative agent of cucumber gray mold, at different butanol extract concentrations. Further analysis of the mutant by real-time PCR and high-performance liquid chromatography revealed increased expression of iturin and surfactin biosynthesis genes as well as enhanced production of iturin and surfactin metabolites. However, the amounts of fengycin obtained from the mutant strain BSM54 were significantly lesser than those of iturin and surfactin. Particularly, iturin A production by the mutant was 3.5-fold higher than that of the wild-type, suggesting that the higher antifungal activity of the mutant against F. oxysporum resulted from the increased expression of biosynthesis genes associated with iturin production. The commercial greenhouse experiment using soil naturally infested with Sclerotinia sclerotiorum (sclerotinia rot) and F. oxysporum (fusarium wilt) showed that the mutant strain reduced sclerotinia rot and fusarium wilt diseases (P = 0.05) more effectively than the wild-type and commercially available product Cillus® in Korea. These results suggest that the mutant with high iturin yield is a potential candidate for the development of a biological control agent in agriculture.

scholarly journals Isolation and characterization of the hydrogenase activity from the non-heterocystous cyanobacteriumSpirulina maxima

FEBS Letters ◽  
1979 ◽  
Vol 98 (2) ◽  
pp. 342-346 ◽  
Author(s):  
María J. Llama ◽  
Juan L. Serra ◽  
K.Krishna Rao ◽  
David O. Hall
Keyword(s):  
Hydrogenase Activity ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of a Listeria monocytogenes mutant strain hyperproducing virulence factors

2006 ◽  
Vol 150 (2) ◽  
pp. 189-195
Author(s):  
Svetlana Ermolaeva ◽  
Nina Varfolomeeva ◽  
Yuri Belyi ◽  
Igor Tartakovskii
Keyword(s):  
Listeria Monocytogenes ◽  
Mutant Strain ◽  
Virulence Factors ◽  
Isolation And Characterization
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