scholarly journals Carbon catabolite repression in plant pathogenic fungi: isolation and characterization of theGibberella fujikuroiandBotrytis cinerea creAgenes

2000 ◽  
Vol 184 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Bettina Tudzynski ◽  
Songjie Liu ◽  
Joan M. Kelly
Keyword(s):  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Pathogenic Fungi ◽  
Plant Pathogenic Fungi ◽  
Isolation And Characterization

Isolation and characterization of dominant mutations resistant to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae

1981 ◽  
Vol 1 (2) ◽  
pp. 83-93
Author(s):  
K Matsumoto ◽  
A Toh-e ◽  
Y Oshima
Keyword(s):  
Partial Resistance ◽  
Carbon Catabolite Repression ◽  
Glucose Repression ◽  
Isocitrate Lyase ◽  
Isolation And Characterization ◽  
Enhanced Resistance ◽  
Leloir Pathway ◽  
Resistant Mutants ◽  
A Site

Seven dominant mutations showing greatly enhanced resistance to the glucose repression of galactokinase synthesis have been isolated from GAL81 mutants, which have the constitutive phenotype but are still strongly repressible by glucose for the synthesis of the Leloir enzymes. These glucose-resistant mutants were due to semidominant mutations at either of two loci, GAL82 and GAL83. Both loci are unlinked to the GAL81- gal4, gal80, or gal7 X gal10 X gal1 locus or to each other. The GAL83 locus was mapped on chromosome V at a site between arg9 and cho1. The GAL82 and GAL83 mutations produced partial resistance of galactokinase to glucose repression only when one or both of these mutations were combined with a GAL81 or a gal80 mutation. The GAL82 and GAL83 mutations are probably specific for expression of the Leloir pathway and related enzymes, because they do not affect the synthesis of alpha-D-glucosidase, invertase, or isocitrate lyase.

scholarly journals BIOLOGICAL CONTROL OF PHYTOPATHOGENIC FUNGI USING DIFFERENT EXTRACTS OF CHAETOMIUM CUPREUM

2018 ◽  
Vol 11 (9) ◽  
pp. 328
Author(s):  
Sharmila Tirumale ◽  
Nazir Wani
Keyword(s):  
Ethyl Acetate ◽  
Mycelial Growth ◽  
Ethyl Acetate Extract ◽  
Pathogenic Fungi ◽  
Phytopathogenic Fungi ◽  
Plant Pathogenic Fungi ◽  
Purification And Characterization ◽  
Butanol Extract ◽  
Ethyl Acetate Extracts

Objective: This study evaluated the Chaetomium cupreum extracts as biocontrol agents against four plant pathogenic fungi (Cladosporium cladosporioides, Fusarium oxysporum, Phomopsis azadirachtae, and Rhizoctonia solani).Method: The antifungal activity of n-butanol and ethyl acetate extracts of C. cupreum was evaluated against plant pathogenic fungi using food poison method.Result: In n-butanol extract, the percentage inhibition of mycelial growth against C. cladosporoides was 88.3±0.1, F. oxysporum was 59.4±0.2, R. solani was 56.2±0.9, and P. azadirachtae was 52.0±0.1at 0.25 mg/ml, respectively. In ethyl acetate extract, the percentage inhibition of mycelial growth against C. cladosporoides was 86.0±0.5, F. oxysporum was 66.4±0.1, P. azadirachtae was 55.2±0.9, and R. solani was 52.0±0.1 at 0.25 mg/ml, respectively.Conclusion: It was found that n-butanol extract is more effective than ethyl acetate extract of C. cupreum. Future studies will focus on the purification and characterization of compounds of C. cupreum and their biocontrol capacity with the mechanism for plant pathological applications.

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