Isolation and characterization of Trichoderma erinaceum for antagonistic activity against plant pathogenic fungi

Objective: This study evaluated the Chaetomium cupreum extracts as biocontrol agents against four plant pathogenic fungi (Cladosporium cladosporioides, Fusarium oxysporum, Phomopsis azadirachtae, and Rhizoctonia solani).Method: The antifungal activity of n-butanol and ethyl acetate extracts of C. cupreum was evaluated against plant pathogenic fungi using food poison method.Result: In n-butanol extract, the percentage inhibition of mycelial growth against C. cladosporoides was 88.3±0.1, F. oxysporum was 59.4±0.2, R. solani was 56.2±0.9, and P. azadirachtae was 52.0±0.1at 0.25 mg/ml, respectively. In ethyl acetate extract, the percentage inhibition of mycelial growth against C. cladosporoides was 86.0±0.5, F. oxysporum was 66.4±0.1, P. azadirachtae was 55.2±0.9, and R. solani was 52.0±0.1 at 0.25 mg/ml, respectively.Conclusion: It was found that n-butanol extract is more effective than ethyl acetate extract of C. cupreum. Future studies will focus on the purification and characterization of compounds of C. cupreum and their biocontrol capacity with the mechanism for plant pathological applications.

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Diversity of cutinases from plant pathogenic fungi: Purification and characterization of two cutinases from Alternaria brassicicola

Physiological and Molecular Plant Pathology ◽

10.1006/pmpp.1993.1019 ◽

1993 ◽

Vol 42 (3) ◽

pp. 205-220 ◽

Cited By ~ 19

Author(s):

Frances Trail ◽

Wolfram Köller

Keyword(s):

Pathogenic Fungi ◽

Alternaria Brassicicola ◽

Plant Pathogenic Fungi ◽

Purification And Characterization

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Isolation and characterization of lactobacilli from human faeces and indigenous fermented foods for their potential application as probiotics

Canadian Journal of Microbiology ◽

10.1139/cjm-2015-0576 ◽

2016 ◽

Vol 62 (4) ◽

pp. 349-359 ◽

Cited By ~ 14

Author(s):

Hemanti Mandal ◽

Ruchi Jariwala ◽

Tamishraha Bagchi

Keyword(s):

Bile Salt ◽

Lactobacillus Rhamnosus ◽

Antagonistic Activity ◽

Food Sources ◽

Fermented Foods ◽

E Coli ◽

Isolation And Characterization ◽

Acid And Bile Tolerance ◽

Ht 29

This study was conducted to select Lactobacillus strains from various sources on the basis of their probiotic attributes, such as acid and bile tolerance, binding to intestinal cells, and antimicrobial activity. Twelve isolates were obtained from human and food sources and were evaluated against standard probiotic Lactobacillus rhamnosus GG (LGG). Isolates were also studied for their antibiotic susceptibility. Isolate Lactobacillus fermentum GPI-6 showed the best survival profile at 0.3% and 1% bile salt, as compared with LGG. Isolates Lactobacillus plantarum GRI-2 and Lactobacillus salivarius GPI-4 showed no reduction in survival rate at pH 2.5. As expected, isolates showed strain-specific differences when comparing various attributes. Isolates GPI-4, GPI-7, and FA-5 showed better adhesion to HT-29, while isolate GPI-4 adhered better to Caco-2 cells than did LGG. However, when studying their ability to compete with Escherichia coli O26:H11, isolates GPI-6 and GPI-7 significantly inhibited E. coli adhesion to both HT-29 and Caco-2 cells compared with LGG. In conclusion, isolates GPI-4, GPI-7, and FA-5 showed excellent binding ability and antagonistic activity and better tolerance to acidic pH (pH 2.5) and to different bile salt concentrations in comparison with LGG, and hence, they could be considered as potential probiotic candidates.

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