scholarly journals Three-dimensional electron microscopic reconstruction of intracellular organellar arrangements in vascular smooth muscle - further evidence of nanospaces and contacts

2009 ◽  
Vol 13 (5) ◽  
pp. 995-998 ◽  
Author(s):  
Wing Chiu Tong ◽  
Michele Sweeney ◽  
Carolyn J. P. Jones ◽  
Henggui Zhang ◽  
Stephen C. O’Neill ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Three Dimensional ◽  
Dimensional Electron ◽  
Electron Microscopic

Neuronal and Extraneuronal Uptake of 3H-Norepinephrine in the Heart of the Active Bat: An Electron Microscopic Radioautographic Study

1973 ◽  
Vol 31 ◽  
pp. 522-523
Author(s):  
Martin Hagopian ◽  
Michael D. Gershon ◽  
Eladio A. Nunez
Keyword(s):  
Smooth Muscle ◽  
Monoamine Oxidase ◽  
Vascular Smooth Muscle ◽  
Cardiac Muscle ◽  
Maximum Rate ◽  
External Medium ◽  
Extraneuronal Uptake ◽  
Electron Microscopic ◽  
Cardiac Tissues ◽  
High Affinity

The ability of cardiac tissues to take up norepinephrine from an external medium is well known. Two mechanisms, called Uptake and Uptake respectively by Iversen have been differentiated. Uptake is a high affinity system associated with adrenergic neuronal elements. Uptake is a low affinity system, with a higher maximum rate than that of Uptake. Uptake has been associated with extraneuronal tissues such as cardiac muscle, fibroblasts or vascular smooth muscle. At low perfusion concentrations of norepinephrine most of the amine taken up by Uptake is metabolized. In order to study the localization of sites of norepinephrine storage following its uptake in the active bat heart, tritiated norepinephrine (2.5 mCi; 0.064 mg) was given intravenously to 2 bats. Monoamine oxidase had been inhibited with pheniprazine (10 mg/kg) one hour previously to decrease metabolism of norepinephrine.

Differentiating human pluripotent stem cells into vascular smooth muscle cells in three dimensional thermoreversible hydrogels

2019 ◽  
Vol 7 (1) ◽  
pp. 347-361 ◽  
Author(s):  
Haishuang Lin ◽  
Qian Du ◽  
Qiang Li ◽  
Ou Wang ◽  
Zhanqi Wang ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Three Dimensional ◽  
Muscle Cells ◽  
Human Pluripotent Stem Cell ◽  
Scalable Production

3D thermoreversible PNIPAAm-PEG hydrogels are used for scalable production of human pluripotent stem cell-derived vascular smooth muscle cells.

Morphology of vascular smooth muscle cells and pericytes as revealed by transmission and scanning EM

1978 ◽  
Vol 36 (2) ◽  
pp. 464-465
Author(s):  
Y. Uehara ◽  
T. Komuro ◽  
J. Desaki
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cell ◽  
Vascular Function ◽  
Three Dimensional ◽  
Muscle Cells ◽  
Structural Level ◽  
Scanning Em

Although subcellular organization of vascular smooth muscle cells and pericytes have been described by a number of authors, the precise overall structure of these cells has not been revealed at the fine structural level.In order to extend our knowledge in understanding of vascular function, the present paper deals the three dimensional organization of these cells as studied with a transmission EM using serial sections and with a scanning EM using materials from which connective tissue elements are removed.For the reconstruction study, arterioles ranging in diameter from l0um to 25um are selected from the hamster skeletal muscle and the rat pancreas.Fig. 1. demonstrates an example of the reconstructed model of the arteriole smooth muscle cell. Here the muscle cell takes a spiral form which coils nearly twice around the endothelial column. The shape and disposition of the muscle cells gradually become to be irregular towards the capillary bed.

A Relatively Inexpensive Microprocessor-Linked Digital Plamimeter for Electron Microscopic Morphometry

1981 ◽  
Vol 39 ◽  
pp. 266-269
Author(s):  
Lee D. Peachey
Keyword(s):  
Theoretical Basis ◽  
Structural Parameters ◽  
Three Dimensional ◽  
Structural Data ◽  
Large Set ◽  
Dimensional Electron ◽  
Electron Microscopic ◽  
Morphometric Methods ◽  
Electron Microscopic Morphometry ◽  
Separate Step

Stereology provides a theoretical basis for powerful morphometric methods for the estimation of three-dimensional structural parameters from two-dimensional electron micrographs of cells and tissues. These methods assume at the start that one has a sufficiently large set of micrographs containing valid structural data. The task of obtaining from these micrographs the large quantity of data needed to get statistically valid results has been eased in two general ways. Sampling of data in the micrograph can be done rapidly by point and intersection counting methods. An alternate method, planimetry, obtains all the data in the micrograph, but in general is more time-consuming than point and intersection counting. Some of the relative inefficiency of planimetry is compensated when a digital planimeter is coupled with a computer. Areas and lengths can be computed simultaneously as fast as profiles are traced. Furthermore, rapid and numerically accurate compilation and statistical analysis of the data can be done automatically as the planimetry is done, not as a separate step after the data have been obtained.

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