scholarly journals Influence of technique and transplantation site on rosette formation in rabbit retinal transplants

2009 ◽  
Vol 75 (1) ◽  
pp. 3-10 ◽  
Author(s):  
R. K. Sharma ◽  
A. Bergström ◽  
B. Ehinger
Keyword(s):  
Rosette Formation ◽  
Retinal Transplants ◽  
Transplantation Site

scholarly journals Transplantation Site Affects the Outcomes of Adipose-Derived Stem Cell-Based Therapy for Retinal Degeneration

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Chengyu Hu ◽  
Huanzhi La ◽  
Xuancheng Wei ◽  
Yue Zhou ◽  
Qingjian Ou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Clinical Trials ◽  
Stem Cell ◽  
Neural Retina ◽  
Rosette Formation ◽  
Subretinal Space ◽  
Electric Response ◽  
Anatomic Structure ◽  
Cell Based Therapy ◽  
Transplantation Site

Adipose-derived stem cells (ASCs) have shown a strong protective effect on retinal degenerative diseases (RDD) after being transplanted into the subretinal space in an animal model. Recently, several clinical trials have been conducted to treat RDD with intravitreal transplantation of stem cells, including ASCs. However, the outcomes of the clinical trials were not satisfactory. To investigate if the transplantation site alters the outcome of stem cell-based therapy for RDD, we isolated rat ASCs (rASCs) and labeled them with green fluorescent protein. Autologous rASCs were grafted into the vitreous chamber or subretinal space in a rat RDD model induced by sodium iodate (SI). The electric response was recorded by ERG. The anatomic structure of the retina was observed in cryosections of rat eyes at posttransplantation weeks 1, 2, and 4. Neural retina apoptosis and epiretinal membrane- (ERM-) like structure formation were investigated by immunostaining. The intravitreal transplantation of rASCs resulted in an extinguished electric response, although the rosette formation and apoptosis of neural retina were reduced. However, the rASCs that grafted in the subretinal space protected the retina from the damage caused by SI, including a partial recovering of the electric response and a reduction in rosette formation. Intravitreally grafted rASCs formed a membrane, resulting in retina folding at the injection site. Müller cells, retinal pigment epithelial cells, and microglial cells migrated from the retina to the rASC-formed membrane and subsequently formed an ERM-like structure. Furthermore, vitreous fluid promoted rASC migration, and rASC-conditioned medium enhanced Müller cell migration as indicated by in vitro studies. These data suggested that the vitreous chamber is not a good transplantation site for ASC-based therapy for RDD and that a deliberate decision should be made before transplantation of stem cells into the vitreous chamber to treat RDD in clinical trials.

Rosette Formation in Hodgkin’s Disease

Oncology ◽  
1974 ◽  
Vol 30 (6) ◽  
pp. 449-457 ◽  
Author(s):  
Alexis Swain ◽  
J.R. Trounce
Keyword(s):  
Hodgkin's Disease ◽  
Hodgkin’S Disease ◽  
Rosette Formation

scholarly journals Histidine-rich glycoprotein blocks T cell rosette formation and modulates both T cell activation and immunoregulation

1989 ◽  
Vol 264 (14) ◽  
pp. 8254-8259
Author(s):  
M Shatsky ◽  
K Saigo ◽  
S Burdach ◽  
L L K Leung ◽  
L J Levitt
Keyword(s):  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Rosette Formation

scholarly journals The Mouse Cornea as a Transplantation Site for Live Imaging of Engineered Tissue Constructs

2010 ◽  
Vol 2010 (4) ◽  
pp. pdb.prot5416-pdb.prot5416 ◽  
Author(s):  
R. A. Poche ◽  
J. E. Saik ◽  
J. L. West ◽  
M. E. Dickinson
Keyword(s):  
Live Imaging ◽  
Tissue Constructs ◽  
Engineered Tissue ◽  
Transplantation Site

Coordinate and independent effects of heroin, cocaine, and alcohol abuse on T-cell E-rosette formation and antigenic marker expression

1986 ◽  
Vol 41 (2) ◽  
pp. 254-264 ◽  
Author(s):  
Robert M. Donahoe ◽  
Janet K.A. Nicholson ◽  
John J. Madden ◽  
Felicia Donahoe ◽  
David A. Shafer ◽  
...  
Keyword(s):  
T Cell ◽  
Alcohol Abuse ◽  
Rosette Formation ◽  
Marker Expression ◽  
Independent Effects

Rosette formation by a mouse fibroblast cell line

Nature ◽  
1974 ◽  
Vol 248 (5448) ◽  
pp. 514-515 ◽  
Author(s):  
E. V. ELLIOTT ◽  
R. S. KERBEL ◽  
B. J. PHILLIPS
Keyword(s):  
Cell Line ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Rosette Formation ◽  
Fibroblast Cell Line ◽  
Mouse Fibroblast Cell ◽  
Mouse Fibroblast Cell Line

Studies on Rosette Formation of Human Lymphocytes with Pig Red Blood Cells

1978 ◽  
Vol 56 (3) ◽  
pp. 273-283 ◽  
Author(s):  
J.S. Smolen ◽  
U. Youngchaiyud ◽  
W.J. Pichler ◽  
M. Binder ◽  
C. Sieffen ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Lymphocytes ◽  
Rosette Formation

Transplantation of Neural Stem/Progenitor Cells at Different Locations in Mice with Spinal Cord Injury

2014 ◽  
Vol 23 (11) ◽  
pp. 1451-1464 ◽  
Author(s):  
Hiroki Iwai ◽  
Satoshi Nori ◽  
Soraya Nishimura ◽  
Akimasa Yasuda ◽  
Morito Takano ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Progenitor Cells ◽  
Functional Recovery ◽  
Control Group ◽  
Cord Injury ◽  
Neural Stem Progenitor Cells ◽  
Neurotropic Factor ◽  
Transplantation Site

Transplantation of neural stem/progenitor cells (NS/PCs) promotes functional recovery after spinal cord injury (SCI); however, few studies have examined the optimal site of NS/PC transplantation in the spinal cord. The purpose of this study was to determine the optimal transplantation site of NS/PCs for the treatment of SCI. Wild-type mice were generated with contusive SCI at the T10 level, and NS/PCs were derived from fetal transgenic mice. These NS/PCs ubiquitously expressed ffLuc-cp156 protein (Venus and luciferase fusion protein) and so could be detected by in vivo bioluminescence imaging 9 days postinjury. NS/PCs (low: 250,000 cells per mouse; high: 1 million cells per mouse) were grafted into the spinal cord at the lesion epicenter (E) or at rostral and caudal (RC) sites. Phosphate-buffered saline was injected into E as a control. Motor functional recovery was better in each of the transplantation groups (E-Low, E-High, RC-Low, and RC-High) than in the control group. The photon counts of the grafted NS/PCs were similar in each of the four transplantation groups, suggesting that the survival of NS/PCs was fairly uniform when more than a certain threshold number of cells were transplanted. Quantitative RT-PCR analyses demonstrated that brain-derived neurotropic factor expression was higher in the RC segment than in the E segment, and this may underlie why NS/PCs more readily differentiated into neurons than into astrocytes in the RC group. The location of the transplantation site did not affect the area of spared fibers, angiogenesis, or the expression of any other mediators. These findings indicated that the microenvironments of the E and RC sites are able to support NS/PCs transplanted during the subacute phase of SCI similarly. Optimally, a certain threshold number of NS/PCs should be grafted into the E segment to avoid damaging sites adjacent to the lesion during the injection procedure.

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