Studies on Plasma Coagulation and Fibrinolysis during Oral Contraception of Various Types With Special Reference to Cold Activation of Factor VII

SummaryThe cold promoted shortening of the thrombotest-times occuring in the plasmas of the majority of women on oral contraception or in the last trimester of pregnancy when incubated overnight at 0°–4° was investigated.The short thrombotest-times were caused by activation of factor VII in a time consuming reaction. Activation was also revealed in the intrinsic coagulation system, but the changes in the activities of coagulation factors other than factor VII were small.Comparison was made between clot promoting effects of cooling and contact, and it was concluded that while contact apparently exerted its main effect in the intrinsic system, cooling predominately activated the extrinsic plasma coagulation system.The cold promoted activation of factor VII seemed to be brought about by an activator.

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Studies on Coagulation and Fibrinolysis in Pregnancy: With Special Reference to Cold Activation of Factor VII

Acta Obstetricia Et Gynecologica Scandinavica ◽

10.3109/00016347509156768 ◽

1975 ◽

Vol 54 (4) ◽

pp. 363-367 ◽

Cited By ~ 15

Author(s):

Halvard Gjønnæss ◽

Magne K. Fagerhol

Keyword(s):

Special Reference ◽

Factor Vii ◽

In Pregnancy ◽

Coagulation And Fibrinolysis

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Changes of Plasma Coagulation and Fibrinolysis in Response to Mental Stress

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646899 ◽

1989 ◽

Vol 62 (02) ◽

pp. 767-771 ◽

Cited By ~ 134

Author(s):

Christina Jern ◽

Elsa Eriksson ◽

Lilian Tengborn ◽

Bo Risberg ◽

Hans Wadenvik ◽

...

Keyword(s):

Plasminogen Activator ◽

Tissue Plasminogen Activator ◽

Mental Stress ◽

Factor Vii ◽

Fibrinogen Concentration ◽

Young Males ◽

Plasma Coagulation ◽

Coagulant Activity ◽

Tissue Plasminogen ◽

Coagulation And Fibrinolysis

SummaryTo study the effects of standardized mental stress (arithmetic and the Stroop color word test) on plasma coagulation and fibrinolysis, blood samples were obtained before, during, and after 20 minutes of mental stress from 10 healthy, non-smoking young males aged 22 to 30 years. Reactions were compared with those observed during physical exercise and infusion of adrenaline. Both von Willebrand factor antigen and factor VIII coagulant activity increased significantly in response to mental stress (95 ± 28 vs 123 + 56%; p <0.05 and 125 ±54 vs 217 ± 170%; p <0.05, respectively). There was also a significant increase of factor VII coagulant activity (86 ± 31 vs 108 + 51%; p <0.05). Furthermore, mental stress caused an activation of the fibrinolytic system with an elevation of tissue plasminogen activator activity and tissue plasminogen activator antigen (0.80 ± 0.48 vs 1.23 ± 0.96 IU/ml; p = 0.076 and 4.38 ± 1.87 vs 5.78 ± 2.58 IU/ml; p <0.01). Fibrinogen concentration increased during stress (1.95 ± 0.29 vs 2.11 ± 0.27 g/1; p <0.05). Similar but more pronounced responses were observed during exercise and adrenaline infusion. Parallel to the increases in coagulation and fibrinolytic factors there were significant increases in heart rate, and systolic and diastolic blood pressure. It is concluded that mental stress has significant effects on plasma coagulation and fibrinolysis, and that it could thus affect important risk factors for cardiovascular disease.

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A study of blood coagulation and fibrinolysis system in experimental acute pancreatitis. With special reference to the effect of antiprotease inhibitor.

The Japanese Journal of Gastroenterological Surgery ◽

10.5833/jjgs.24.985 ◽

1991 ◽

Vol 24 (4) ◽

pp. 985-992

Author(s):

Sin-Soo Ha ◽

Katsusuke Satake ◽

Akihito Hiura ◽

Hideki Nishiwaki ◽

Kaoru Umeyama

Keyword(s):

Acute Pancreatitis ◽

Special Reference ◽

Blood Coagulation ◽

Experimental Acute Pancreatitis ◽

Coagulation And Fibrinolysis

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Involvement Of Human Factor IX In Tissue Thromboplastin Induced Coagulation

10.1055/s-0038-1652050 ◽

1981 ◽

Author(s):

A M H P van den Besselaar ◽

I E Ram ◽

R M Bertina

Keyword(s):

Tissue Factor ◽

Polyacrylamide Gel Electrophoresis ◽

Fold Increase ◽

Factor Ix ◽

Factor Vii ◽

Factor Xii ◽

Free System ◽

Plasma Coagulation ◽

Human Factor Ix ◽

Tissue Thromboplastin

This study is concerned with the question whether activation of factor IX by factor VII - tissue thromboplastin contributes to the rate of plasma coagulation. The protein component of tissue factor was partially purified from human brain. Its molecular weight as deduced from SDS - polyacrylamide gel electrophoresis was about 48,000. Reconstitution of thromboplastin activity was obtained by mixing apoprotein and phospholipids in the presence of Triton X-100 and subsequent removal of Triton by adsorption to Biobeads SM-2. Reconstituted tissue factor greatly accelerated the activation of factor IX by isolated factor VII in the presence of calcium ions. In a contact free system (plasma from a patient with congenital factor XII deficiency; factor XII<0.001 Unit/ml) plasma coagulation times (tc) were determined as a function of apoprotein concentration (at constant phospholipid) both in the presence and absence of factor IX. At high apoprotein concentration tc showed to be independent of factor IX, whereas at low apoprotein concentration the removal of factor IX resulted in a 2 - 3 fold increase of tc. The involvement of the tissue factor - factor VII complex in this phenomenon was evaluated using a specific anti-factor VII serum. The results indicate that activation of factor IX by factor VII - tissue thromboplastin does not significantly contribute to the rate of plasma coagulation.

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LOCAL ACTIVATION OF COAGULATION AND FIBRINOLYSIS IN LUNG DISEASE IS REFLECTED IN BR0NCH0ALVE0LAR LAVAGE FLUID

10.1055/s-0038-1643054 ◽

1987 ◽

Author(s):

Britt Nakstad ◽

Torstein Lyberg

Keyword(s):

Lung Disease ◽

Degradation Products ◽

Membrane Vesicles ◽

Lavage Fluid ◽

Factor Vii ◽

Cell Protein ◽

Factor X ◽

Fibrin Degradation Products ◽

Activation Products ◽

Coagulation And Fibrinolysis

Fibrin deposition in the alveolar space and the lung interstitium is a prominent feature of many types of pulmonary inflammatory diseases.Ce11s of the monocyte/ macrophage lineage have a dualistic role in the balance between coagulation and fibrinolysis having the ability to synthesize both procoagulant factors and plasminogen activators. In the present studies bronchoa1veo1 ar lavage were performed on 128 patients with various lung diseases and 29 healthy controls.Both lung alveolar macrophages (LAM) and the supernatant bronchoalveolar lavage fluid (BALF) expressed two types of procoagulant activities a)thromboplastin and b) a direct factor X activator.The procoagulant in BALF was associated with membrane vesicles which sedimented at 100000g,lh.By electron microscopy the BALF ultrasediment was seen to consist for a large part of membrane material and this was confirmed by monitoring the content of different marker enzymes for specific subcellular structures. LAM from patients had significantly higher specific thromboplastin activity than LAM from controls (4,36 -0.98(SEM)vs.0.81± 0.14 U/mg cell protein).BALF collected from patients had significantly higher levels than BALF from controls of a ) thrombop1 astin(0.66-0.18vs.0.07 ±0.01 U/ml) b ) factor VII activity(1.33±0.31vs.0.48±0.06 U/ml) c)fibrin degradation products(presentin 28,7vs.0% of the cases) and d) fibronectin(491±103 vs.35±5 ng/ml ) In addition,the level of plasminogen activator was higher in controls than in patients(294±68 vs.!02±14 m U/m1).These studies show that activation products of the coagulation and fibrinolytic systems can be detected in BALF and that lung disease often is associated with abberations in the balance between these systems.Fibrin serves as a substrate for fibronectin and the increases in lavage fibronectin may reflect the development of lung fibrosis.

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