scholarly journals Genomic constitution of Elymus parviglumis and E. pseudonutans: Triticeae (Poaceae)

Hereditas ◽  
2008 ◽  
Vol 113 (2) ◽  
pp. 109-119 ◽  
Author(s):  
BAO-RONG LU ◽  
ROLAND VON BOTHMER
Keyword(s):  
Genomic Constitution

scholarly journals Wheat, Rye, and Barley Genomes Can Associate during Meiosis in Newly Synthesized Trigeneric Hybrids

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 113
Author(s):  
María-Dolores Rey ◽  
Carmen Ramírez ◽  
Azahara C. Martín
Keyword(s):  
Chromosome Segregation ◽  
Genome Duplication ◽  
Triticum Turgidum ◽  
Aegilops Tauschii ◽  
Genomic In Situ Hybridization ◽  
Hordeum Chilense ◽  
Genomic Constitution ◽  
Chromosome Associations ◽  
High Level

Polyploidization, or whole genome duplication (WGD), has an important role in evolution and speciation. One of the biggest challenges faced by a new polyploid is meiosis, in particular, discriminating between multiple related chromosomes so that only homologs recombine to ensure regular chromosome segregation and fertility. Here, we report the production of two new hybrids formed by the genomes of species from three different genera: a hybrid between Aegilops tauschii (DD), Hordeum chilense (HchHch), and Secale cereale (RR) with the haploid genomic constitution HchDR (n = 7× = 21); and a hybrid between Triticum turgidum spp. durum (AABB), H. chilense, and S. cereale with the constitution ABHchR (n = 7× = 28). We used genomic in situ hybridization and immunolocalization of key meiotic proteins to establish the chromosome composition of the new hybrids and to study their meiotic behavior. Interestingly, there were multiple chromosome associations at metaphase I in both hybrids. A high level of crossover (CO) formation was observed in HchDR, which shows the possibility of meiotic recombination between the different genomes. We succeeded in the duplication of the ABHchR genome, and several amphiploids, AABBHchHchRR, were obtained and characterized. These results indicate that recombination between the genera of three economically important crops is possible.

Genomic constitution of Festuca × Lolium hybrids revealed by the DArTFest array

2010 ◽  
Vol 122 (2) ◽  
pp. 355-363 ◽  
Author(s):  
David Kopecký ◽  
Jan Bartoš ◽  
Pavla Christelová ◽  
Vladimír Černoch ◽  
Andrzej Kilian ◽  
...  
Keyword(s):  
Genomic Constitution

scholarly journals GISH analysis revealed new aspect of genomic constitution of Thinopyrum intermedium

2012 ◽  
Vol 41 (Special Issue) ◽  
pp. 92-95 ◽  
Author(s):  
M. Kishii ◽  
R.R.-C. Wang ◽  
H. Tsujimoto
Keyword(s):  
Full Text ◽  
Thinopyrum Intermedium ◽  
Genomic Constitution ◽  
Gish Analysis

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Study of the genomic constitution of the German mirror carp

2014 ◽  
Vol 23 (1) ◽  
pp. 81-91
Author(s):  
Ya-Juan Li ◽  
Bo Liu ◽  
He Zhou ◽  
Yang-Chun Gao ◽  
Min Gao ◽  
...  
Keyword(s):  
Genomic Constitution ◽  
Mirror Carp

scholarly journals Heterozygous indels as useful tools in the reconstruction of DNA sequences and in the assessment of ploidy level and genomic constitution of hybrid organisms

DNA Sequence ◽  
2005 ◽  
Vol 16 (6) ◽  
pp. 462-467 ◽  
Author(s):  
Carla Sousa-Santos ◽  
Joana I. Robalo ◽  
Maria-João Collares-Pereira ◽  
Vitor C. Almada
Keyword(s):  
Ploidy Level ◽  
Dna Sequences ◽  
Genomic Constitution

The genome composition of hexaploid Psammopyrum athericum and octoploid Psammopyrum pungens (Poaceae: Triticeae)

Genome ◽  
2003 ◽  
Vol 46 (1) ◽  
pp. 164-169 ◽  
Author(s):  
Pernilla Ellneskog-Staam ◽  
Björn Salomon ◽  
Roland von Bothmer ◽  
Kesara Anamthawat-Jónsson
Keyword(s):  
In Situ Hybridization ◽  
Genome Analysis ◽  
Genomic In Situ Hybridization ◽  
Agropyron Cristatum ◽  
Genome Composition ◽  
Genomic Constitution ◽  
Hybridization Pattern ◽  
Genomic Probe

The genomic constitution of two species in the genus Psammopyrum, i.e., Ps. athericum (2n = 6x = 42) and Ps. pungens (2n = 8x = 56), was studied by genomic in situ hybridization (GISH). In Ps. athericum, one diploid chromosome set hybridized to a genomic probe from Pseudoroegneria ferganensis (St genome), one diploid set to a probe from Agropyron cristatum (P genome), and one diploid set to a probe from Thinopyrum junceiforme (EbEe genomes) or Th. bessarabicum (Eb genome). Substituting the St-genome probe with an L-genome probe from Festucopsis serpentinii resulted in exactly the same hybridization pattern, suggesting a genomic constitution of EStP or ELP for Ps. athericum. The same probes used on Ps. pungens showed two diploid sets of chromosomes hybridizing to the St-genome probe, one diploid set hybridizing to the P-genome probe, and one diploid set hybridizing to the EbEe-genome probe. The L-genome probe hybridized to approximately 14 of the chromosomes that were labeled by the St-genome probe. Hence the genomic constitution for Ps. pungens is proposed to be EStStP or EStLP.Key Words: Psammopyrum athericum, Psammopyrum pungens, in situ hybridization, Elytrigia pycnantha, Elytrigia pungens, genome analysis.

The genomic composition of Tricepiro, a synthetic forage crop

Genome ◽  
2005 ◽  
Vol 48 (1) ◽  
pp. 154-159 ◽  
Author(s):  
María Rosa Ferrari ◽  
Eduardo J Greizerstein ◽  
Héctor A Paccapelo ◽  
Carlos A Naranjo ◽  
Angelines Cuadrado ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Chromosomal Location ◽  
Hexaploid Triticale ◽  
Genomic Constitution ◽  
Forage Crop ◽  
A Genome ◽  
Total Dna ◽  
Synthetic Hexaploid ◽  
Specific Sequences

Chromosome in situ hybridization (FISH and GISH) is a powerful tool for determining the chromosomal location of specific sequences and for analysing genome organization and evolution. Tricepiro (2n = 6x = 42) is a synthetic cereal obtained by G. Covas in Argentina (1972), which crosses hexaploid triticale (2n = 6x = 42) and octoploid Trigopiro (2n = 8x = 56). Several years of breeding produced a forage crop with valuable characteristics from Secale, Triticum, and Thinopyrum. The aim of this work is to analyse the real genomic constitution of this important synthetic crop. In situ hybridization using total DNA of Secale, Triticum, and Thinopyrum as a probe (GISH) labelled with biotin and (or) digoxigenin showed that tricepiro is composed of 14 rye chromosomes and 28 wheat chromosomes. Small zones of introgression of Thinopyrum on wheat chromosomes were detected. The FISH using the rye repetitive DNA probe pSc 119.2 labelled with biotin let us characterize the seven pairs of rye chromosomes. Moreover, several wheat chromosomes belonging to A and B genomes were distinguished. Therefore, tricepiro is a synthetic hexaploid (2n = 6x = 42) being AABBRR in its genomic composition, with zones of introgression of Thinopyrum in the A genome of wheat.Key words: tricepiro, trihybrid, Triticum, Secale, Thinopyrum, in situ hybridization, FISH, GISH, genomic composition, synthetic forage crop.

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