Identification of a foreign body - tick (Ixodes) - with confocal laser scan microscopy (CLSM) in comparison to histology in a mouse model

2012 ◽  
Vol 10 (4) ◽  
pp. 277-278 ◽  
Author(s):  
Sezgin Erdoğan ◽  
Peter Dorittke ◽  
Bernd Kardorff
Keyword(s):  
Foreign Body ◽  
Mouse Model ◽  
Confocal Laser ◽  
Confocal Laser Scan Microscopy

Mouse model of foreign body reaction that alters the submesothelium and transperitoneal transport

2011 ◽  
Vol 300 (1) ◽  
pp. F283-F289 ◽  
Author(s):  
Toni Peters ◽  
Rebecca Potter ◽  
Xiarong Li ◽  
Zhi He ◽  
Glenn Hoskins ◽  
...  
Keyword(s):  
Foreign Body ◽  
Mouse Model ◽  
Foreign Body Reaction ◽  
Cell Layer ◽  
Adherent Cell ◽  
Transfer Coefficients ◽  
Tissue Samples ◽  
C57bl Mice ◽  
Strong Staining ◽  
Filtration Flux

To address the hypothesis that sterile intraperitoneal (ip) catheters alone promote a progressive foreign body reaction (FBR), silicone catheters were surgically implanted in C57BL mice. Controls (CON) underwent sham operations. After 1–5 wk (E1–E5 for catheter-bearing mice), catheters were recovered, and the adherent cell layer (ACL) was separated and cultured to demonstrate sterility. Transperitoneal transport experiments were performed to determine the mass transfer coefficients of mannitol (MTCM) and albumin (MTCA) and the osmotic filtration flux ( Josm). After euthanasia, tissue samples were analyzed for submesothelial thickness, angiogenesis, and cytokine immunohistochemistry (IHC). Progressive increases with time were observed in submesothelial thickness (μm: CON, 18.8 ± 12.3; E1, 46.1 ± 20.0; E2, 72.0 ± 17.9; E4, 97.3 ± 20.0; E5, 131.7 ± 10.3; P < 0.003), angiogenesis (no. of vessels/mm of peritoneum: CON, 10.7 ± 9.4; E1, 15.4 ± 15.6; E2, 27.0 ± 14.0; E4, 39.8 ± 15.7; E5, 90.1 ± 8.1; P < 0.0003), MTCA(6.5 ± 1.5 × 10−5cm/min, mean CON; 18.0 ± 1.1 × 10−5cm/min, mean E1–E5, P < 0.0001), Josm(0.0013 ± 0.0001 cm/min, mean CON; 0.0017 ± 0.0001 cm/min, mean E1–E5, P < 0.01). No significant differences were found for MTCM. IHC demonstrated strong staining for all treated animals and correlated with the ACL. This mouse model demonstrates that ip silicone catheters result in progressive FBR, altering the submesothelial anatomy and transperitoneal transport, and will form the basis for mechanistic studies in genetically-altered animals.

Endoscopy and Confocal Laser Endomicroscopy: A Validation Study in a Mouse Model of Colitis

2011 ◽  
Vol 140 (5) ◽  
pp. S-651
Author(s):  
Rae R. Foshaug ◽  
Aducio Thiesen ◽  
Karen I. Kroeker ◽  
Thomas W. Lee ◽  
Karen Wong ◽  
...  
Keyword(s):  
Mouse Model ◽  
Validation Study ◽  
Confocal Laser Endomicroscopy ◽  
Confocal Laser

Natural History of Chronic Staphylococcus epidermidis Foreign Body Infection in a Mouse Model

1991 ◽  
Vol 164 (6) ◽  
pp. 1220-1223 ◽  
Author(s):  
B. Gallimore ◽  
R. F. Gagnon ◽  
R. Subang ◽  
G. K. Richards
Keyword(s):  
Foreign Body ◽  
Natural History ◽  
Mouse Model ◽  
Staphylococcus Epidermidis ◽  
History Of

Expression of the gap junction proteins connexin31 and connexin43 correlates with communication compartments in extraembryonic tissues and in the gastrulating mouse embryo, respectively

1996 ◽  
Vol 109 (1) ◽  
pp. 191-197
Author(s):  
E. Dahl ◽  
E. Winterhager ◽  
B. Reuss ◽  
O. Traub ◽  
A. Butterweck ◽  
...  
Keyword(s):  
Mouse Embryo ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Confocal Laser ◽  
Mouse Development ◽  
Confocal Laser Scan Microscopy ◽  
Early Mouse ◽  
Junction Proteins ◽  
In Cells ◽  
Extraembryonic Tissues

We have characterized the pattern of connexin expression in embryonic and extraembryonic tissues during early mouse development. In the preimplantation blastocyst, at 3.5 days post coitum (dpc), immunofluorescent signals specific for connexin31 and connexin43 proteins were present in both the inner cell mass and the trophectoderm, as shown by confocal laser scan microscopy. Immediately after implantation at 6.5 dpc, however, we find complete compartmentation of these two connexins: connexin31 mRNA and protein are expressed exclusively in cells derived from the trophectoderm lineage, whereas connexin43 mRNA and protein are detected in cells derived from the inner cell mass. This expression pattern of connexin31 and connexin43 is maintained at 7.5 dpc when the axial polarity of the mouse embryo is established. It correlates with the communication compartments in extraembryonic tissues and the gastrulating mouse embryo, respectively. The communication boundary between those compartments may be due to incompatibility of connexin31 and connexin43 hemichannels, which do not communicate with each other in cell culture.

scholarly journals Ganoderic Acid A Promotes Amyloid-β Clearance (In Vitro) and Ameliorates Cognitive Deficiency in Alzheimer’s Disease (Mouse Model) Through Autophagy Induced by Activating Axl

2021 ◽  
Vol 22 (11) ◽  
pp. 5559
Author(s):  
Li-Feng-Rong Qi ◽  
Shuai Liu ◽  
Yu-Ci Liu ◽  
Ping Li ◽  
Xiaojun Xu
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Mouse Model ◽  
Amyloid Β ◽  
Microglial Cells ◽  
Confocal Laser ◽  
Ganoderic Acid ◽  
Aβ Clearance ◽  
Cognitive Deficiency ◽  
Ad Mouse Model

Alzheimer’s disease (AD) is thought to be caused by amyloid-β (Aβ) accumulation in the central nervous system due to deficient clearance. The aim of the present study was to investigate the effect of ganoderic acid A (GAA) on Aβ clearance in microglia and its anti-AD activity. Aβ degradation in BV2 microglial cells was determined using an intracellular Aβ clearance assay. GAA stimulated autophagosome formation via the Axl receptor tyrosine kinase (Axl)/RAC/CDC42-activated kinase 1 (Pak1) pathway was determined by Western blot analyses, and fluorescence-labeled Aβ42 was localized in lysosomes in confocal laser microscopy images. The in vivo anti-AD activity of GAA was evaluated by object recognition and Morris water maze (MWM) tests in an AD mouse model following intracerebroventricular injection of aggregated Aβ42. The autophagy level in the hippocampus was assayed by immunohistochemical assessment against microtubule-associated proteins 1A/1B light-chain 3B (LC3B). Intracellular Aβ42 levels were significantly reduced by GAA treatment in microglial cells. Additionally, GAA activated autophagy according to increased LC3B-II levels, with this increased autophagy stimulated by upregulating Axl and Pak1 phosphorylation. The effect of eliminating Aβ by GAA through autophagy was reversed by R428, an Axl inhibitor, or IPA-3, a Pak1 inhibitor. Consistent with the cell-based assay, GAA ameliorated cognitive deficiency and reduced Aβ42 levels in an AD mouse model. Furthermore, LC3B expression in the hippocampus was up-regulated by GAA treatment, with these GAA-specific effects abolished by R428. GAA promoted Aβ clearance by enhancing autophagy via the Axl/Pak1 signaling pathway in microglial cells and ameliorated cognitive deficiency in an AD mouse model.

scholarly journals Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cells

2005 ◽  
Vol 48 (3) ◽  
pp. 351-357 ◽  
Author(s):  
Celso Aparecido Polinarski ◽  
José Luis da Conceição Silva ◽  
Liya Regina Mikami ◽  
Maria Aparecida Fernandez
Keyword(s):  
Salivary Gland ◽  
Structure And Function ◽  
Confocal Laser ◽  
Work Analysis ◽  
Confocal Laser Scan Microscopy ◽  
Nuclear Halo ◽  
Hypotonic Buffer ◽  
And Function ◽  
Polytene Nuclei ◽  
Bradysia Hygida

A protocol for recovered nuclear halos from insect polytene nuclei after the extraction of the nuclear proteins using LIS detergent is reported in this work. Analysis was carried out using fluorescence and confocal laser scan microscopy. The extraction of nuclear halos was possible only with nuclei-fraction isolation in hypotonic buffer without spermine and spermidine. The recovered nuclear halos from Bradysia hygida salivary gland polytene nuclei, contributed greatly to the study of the structure and function of these special organelles.

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