Bile Salt-Stimulated Lipase Activity in Human Colostrum from Mothers of Infants of Different Gestational Age and Birthweight

Time-temperature relationships for heat-inactivation of the bile salt-stimulated lipase activity were compared in whole human milk and in the same product fortified to 9 mM/ml with sodium taurocholate. Heat treatments were varied from 45 to 70°C for times ranging from 15s to 40 min. Enzyme activity was more heat stable in human milk fortified with taurocholate than in control samples. The temperature required for the onset of heat inactivation at 30-min holding time was increased from 45°C for control samples to 60°C following addition of taurocholate. A temperature differential of approximately 12°C was required in the fortified milks to produce inactivation equivalent to that observed in the control milks over the heating range studied.

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Heat Inactivation of Bile Salt-Stimulated Lipase Activity in Human Milk and Colostrum1

Journal of Food Protection ◽

10.4315/0362-028x-46.6.525 ◽

1983 ◽

Vol 46 (6) ◽

pp. 525-527

Author(s):

S. H. L. PAN ◽

C. W. DILL ◽

E. S. ALFORD ◽

R. L. RICHTER ◽

C. GARZA

Keyword(s):

Human Milk ◽

Bile Salt ◽

Lipase Activity ◽

Bovine Milk ◽

Heat Inactivation ◽

Assay Procedure ◽

Ph Stat

Time-temperature relationships for heat-inactivation of bile salt-stimulated lipase activity in human milk and colostrum were systematically measured using a pH-stat assay procedure with triolein as substrate. The enzyme was not affected in either menstruum at 45°C for 40 min. The enzyme was destroyed almost instantaneously at 60°C, and was slightly more heat-sensitive in colostrum than in milk. The bile salt-stimulated lipase(s) in human milk was more heat sensitive than lipase in bovine milk.

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Effect of dietary taurine supplementation on growth performance and bile salt activated lipase activity of common dentex, Dentex dentex, fed a fish meal/soy protein concentrate-based diet

Aquaculture ◽

10.1016/j.aquaculture.2007.12.013 ◽

2008 ◽

Vol 275 (1-4) ◽

pp. 201-208 ◽

Cited By ~ 116

Author(s):

Stavros Chatzifotis ◽

Irene Polemitou ◽

Pascal Divanach ◽

Efthimia Antonopoulou

Keyword(s):

Bile Salt ◽

Growth Performance ◽

Soy Protein ◽

Lipase Activity ◽

Fish Meal ◽

Soy Protein Concentrate ◽

Protein Concentrate ◽

Taurine Supplementation ◽

Dentex Dentex

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Effect of gestational age (preterm or full term) on lipid composition of the milk fat globule and its membrane in human colostrum

Journal of Dairy Science ◽

10.3168/jds.2020-18428 ◽

2020 ◽

Vol 103 (9) ◽

pp. 7742-7751 ◽

Cited By ~ 1

Author(s):

Antonio Pérez-Gálvez ◽

María V. Calvo ◽

Javier Megino-Tello ◽

Josefa Aguayo-Maldonado ◽

Rafael Jiménez-Flores ◽

...

Keyword(s):

Gestational Age ◽

Lipid Composition ◽

Milk Fat ◽

Full Term ◽

Human Colostrum ◽

Milk Fat Globule ◽

Fat Globule

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Lipolytic Activity During Storage of Human Milk: Stability of the Bile Salt-Stimulated Lipase1

Journal of Food Protection ◽

10.4315/0362-028x-46.11.994 ◽

1983 ◽

Vol 46 (11) ◽

pp. 994-996 ◽

Cited By ~ 2

Author(s):

C. W. DILL ◽

C. T. CHEN ◽

E.S. ALFORD ◽

R. L. EDWARDS ◽

R. L. RICHTER ◽

...

Keyword(s):

Human Milk ◽

Bile Salt ◽

Lipase Activity ◽

Room Temperature ◽

Lipolytic Activity ◽

Skim Milk ◽

C Storage ◽

Whole Milk ◽

Freeze Dried ◽

Initial Drop

Bile salt-stimulated lipase activity was monitored in fresh human milk and skim milk during refrigerated (4°C) and frozen (−20°C) storage, and in the lyophilized milks stored at −20°C and at room temperature. Following a sharp initial drop to approximately 77% of the original lipase activity, lipase was relatively stable in frozen or freeze-dried milks during 180 d of storage at −20°C. Activity losses were greatest (P < .05) in freeze-dried whole milks and skim milks stored at room temperature, approximating a 30% loss during 30 d of storage. Lipase activity was stable during refrigerated (4°C) storage of whole milk for 1 week.

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