GRAFT-VERSUS-HOST REACTIONS MEDIATED BY A THYMUS-BONE MARROW COMBINATION, SPLEEN CELLS, AND LYMPH NODE CELLS FROM AMYLOIDOTIC AND NONAMYLOIDOTIC MICE

A mortality assay was used to quantitate graft-versus-host (GVH) reactions in sublethally irradiated (400 R) neonatal (C57BL/6 x BALB/c)F1 recipients of BALB/c lymphoid cells from various tissues. The probit of the 35 day cumulative per cent of mortality was a linear function of the logarithm of the cell inoculum for any tissue; reactivities of different tissues fell on a series of parallel lines. Peripheral blood leukocytes (PBL), the most active cells, were about 30 times as active as thymocytes, the least active cells studied; femoral lymph node cells and spleen cells were about 23 and 8 times as reactive as thymocytes, respectively. The average survival time of recipients of thymocytes who eventually died was nearly a week longer than that of recipients of comparably lethal numbers of PBL, lymph node, or spleen cells. Mixtures of PBL and thymocytes gave levels of 35 day mortality significantly greater than those expected if the reactivities of the mixture had been merely the sum of the reactivities of the components measured separately, thereby confirming in any assay independent of host splenomegaly the synergistic interaction of thymocytes and PBL in the GVH reaction. Both populations of cells in the mixture had to be allogeneic to the host in order to observe this synergy. The kinetics of cumulative mortality observed for mixtures of PBL and thymocytes were indistinguishable from those seen with thymocytes alone, indicating activation of the latter cell type. Finally, comparison of the relative abilities of different cell populations to cause splenomegaly on the one hand and lethal runting on the other has raised the possibility that expression of different effector functions of cell-mediated immune reactions may in fact be initiated by distinct cells.

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TRANSFER OF RESPONSIVENESS TO HAPTEN CONJUGATES OF POLY-L-LYSINE AND OF A COPOLYMER OF L-GLUTAMIC ACID AND L-LYSINE TO LETHALLY IRRADIATED NON-RESPONDER GUINEA PIGS BY BONE MARROW OR LYMPH NODE AND SPLEEN CELLS FROM RESPONDER GUINEA PIGS

Journal of Experimental Medicine ◽

10.1084/jem.130.5.1107 ◽

1969 ◽

Vol 130 (5) ◽

pp. 1107-1122 ◽

Cited By ~ 23

Author(s):

John Foerster ◽

Ira Green ◽

Jean-Pierre Lamelin ◽

Baruj Benacerraf

Keyword(s):

Bone Marrow ◽

Immune Response ◽

Lymph Node ◽

Glutamic Acid ◽

Graft Versus Host Disease ◽

Guinea Pigs ◽

Allogeneic Bone ◽

Spleen Cells ◽

Host Disease ◽

Graft Versus Host

Hartley guinea pigs genetically unresponsive to hapten-PLL (poly-L-lysine) conjugates were lethally irradiated and given allogeneic bone marrow from Hartley responder animals. Many of the animals died of graft versus host disease before their response to 2,4-dinitrophenyl-PLL (DNP-PLL) could be measured. The immune response of the surviving recipient animals was evaluated by anti-DNP antibody production, development of delayed hypersensitivity to DNP-poly-L-lysine, as well as by lymph node cell stimulation in vitro by this antigen. 12 of 14 recipient animals thus treated made an immune response as measured by 2 of the 3 parameters. Strain 13 guinea pigs, genetically unable to respond immunologically to DNP-PLL and to DNP-GL (2,4-dinitrophenyl-L-glutamic acid L-lysine copolymer) were lethally irradiated and given bone marrow from (2 x 13) F1 responder animals or strain 13 bone marrow and (2 x 13) F1 lymph node and spleen cells. A high proportion of the animals survived this procedure; no evidence of graft versus host disease was observed. Three of three strain 13 animals irradiated and, given strain 13 bone marrow and (2 x 13) F1 lymph node and spleen, and then immunized with DNP-PL, made a specific immune response. 7 of 10 irradiated strain 13 animals given strain 13 bone marrow and (2 x 13) F1 lymph node and spleen made an immune response to DNP-GL. However, only one of six irradiated strain 13 animals made a vigorous immune response to DNP-GL after reconstitution with (2 x 13) F1 bone marrow alone. The ability to transfer the immune response to PLL antigens from responder to nonresponder animals demonstrates unequivocally that the defect in the non-responder animals is immunological rather than due to some other type of non-immunological mechanism. The bone marrow contains all the immunological cells necessary for the expression of the PLL gene. However, the finding that (2 x 13) F1 lymph node and spleen cells were more effective than (2 x 13)F1 bone marrow cell populations (known to be a rich source of monocyte precursors) suggests that the cells in which the PLL gene function is expressed may be lymphocytes rather than monocytes and macrophages.

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Evidence for the involvement of a bone marrow-derived cell population in the immune expulsion ofTrichinella spiralis

Parasitology ◽

10.1017/s0031182000047855 ◽

1977 ◽

Vol 74 (3) ◽

pp. 225-234 ◽

Cited By ~ 15

Author(s):

D. Wakelin ◽

Margaret M. Wilson

Keyword(s):

Bone Marrow ◽

Lymph Node ◽

Cell Population ◽

Mesenteric Lymph Node ◽

Trichinella Spiralis ◽

Mesenteric Lymph ◽

Lymph Node Cells

When mice were irradiated immediately before infection withTrichinella spiralisthere was a profound and long-lasting interference with their ability to expel adult worms from the intestine. Irradiation given after the fifth day of infection was progressively less effective in this respect. The ability to expel worms was not restored when mesenteric lymph node cells (MLNC) were transferred (a) on the day of infection in mice irradiated one day previously, or (b) on day 7 of an infection in mice irradiated on day 6, even though the MLNC transferred immunity to intact recipients. Transfer of bone marrow (BM) alone was also without effect. However, worm explusion was restored if, following irradiation and injection of BM, 10 days were allowed for BM differentiation before transfer of MLNC. This restoration was effective even after lethal levels of irradiation and was clearly dependent upon a donor-derived BM component cooperating with, or responding to, the activity of the transferred MLNC. The possibility that the BM component is non-lymphoid in nature is discussed.

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Truncated mu (mu') chains in murine IgM. Evidence that mu' chains lack variable regions.

Journal of Experimental Medicine ◽

10.1084/jem.162.6.1862 ◽

1985 ◽

Vol 162 (6) ◽

pp. 1862-1877 ◽

Cited By ~ 15

Author(s):

R Marks ◽

M J Bosma

Keyword(s):

Bone Marrow ◽

Lymph Node ◽

Molecular Mass ◽

Tumor Cells ◽

Isoelectric Focusing ◽

Bone Marrow Cells ◽

Normal Serum ◽

Antigenic Determinants ◽

Variable Regions ◽

Lymph Node Cells

Secreted IgM was shown to contain truncated mu (mu') chains with an apparent molecular mass of approximately 55 kD. The estimated percentage of IgM heavy (H) chains in the mu' form ranged from less than or equal to 1% in the case of one tumor IgM protein (104E) to greater than or equal to 30% in normal serum IgM. Serum mu' chains lacked antigenic determinants characteristic of immunoglobulin variable regions and showed a restricted isoelectric focusing pattern compared with that of conventional mu chains. Intracellular mu' chains were readily detected in bone marrow cells but not in spleen or lymph node cells; mu' chains were also detected in IgM-producing tumor cells and in a hybridoma cell line that deleted its productive mu allele. These results predict irregularities in IgM structure and recall an old controversy concerning the valence of IgM molecules.

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Influence of red bone marrow allotransplantation on neuron-specific (NSE) and histamine-containing lymph node cells

Cell Technologies in Biology and Medicine ◽

10.47056/1814-3490-2021-4-246-249 ◽

2021 ◽

pp. 246-249

Author(s):

V. O. Romanov ◽

◽

L. A. Lyubovtseva ◽

O. V. Vorob'eva ◽

L. P. Romanova ◽

...

Keyword(s):

Bone Marrow ◽

Lymph Node ◽

Red Bone Marrow ◽

Lymph Node Cells

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A Method of Reducing the Incidence of the Secondary Syndrome in Allogenic Marrow Transplantation

Blood ◽

10.1182/blood.v22.1.44.44 ◽

1963 ◽

Vol 22 (1) ◽

pp. 44-52 ◽

Cited By ~ 17

Author(s):

GEORGES MATHÉ ◽

JEAN-LOUIS AMIEL ◽

LÉON SCHWARZENBERG ◽

ANNE-MARIE MERY ◽

F. Lapeyraque

Keyword(s):

Bone Marrow ◽

Lymph Node ◽

Marrow Transplantation ◽

Clinical Medicine ◽

Bone Marrow Cells ◽

Allogenic Bone ◽

Allogenic Bone Marrow ◽

Lymph Node Cells ◽

Bone Marrow Grafting ◽

Marrow Cells

Abstract Preservation of a graft of lymph node cells or semi-allogenic bone marrow cells at 37 C. in Tyrode’s solution for 2 hours, which reduces the percentage of cells not permeable to eosin by half, has a statistically significant reducing effect on the frequency of acute or chronic secondary syndromes which occur in irradiated recipients. Preservation at 18 C. for 6 hours, which in the same way increases the percentage of cells permeable to eosin, does not have the same effect. These two methods of preserving bone marrow cells do not appreciably reduce the myeloid-restoring capacity of compatible or incompatible irradiated recipients. Application of these results to bone marrow grafting in clinical medicine is discussed.

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Mortality of Radiation Chimeras in Relation to the Number of Transplanted Bone Marrow and Lymph Node Cells2

JNCI Journal of the National Cancer Institute ◽

10.1093/jnci/36.3.431 ◽

1966 ◽

Keyword(s):

Bone Marrow ◽

Lymph Node ◽

Radiation Chimeras ◽

Lymph Node Cells

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BONE MARROW AS SOURCE OF CELLS IN REACTIONS OF CELLULAR HYPERSENSITIVITY

Journal of Experimental Medicine ◽

10.1084/jem.128.6.1437 ◽

1968 ◽

Vol 128 (6) ◽

pp. 1437-1449 ◽

Cited By ~ 32

Author(s):

David M. Lubaroff ◽

Byron H. Waksman

Keyword(s):

Bone Marrow ◽

Lymph Node ◽

Lymph Nodes ◽

Skin Test ◽

Skin Tests ◽

Skin Reactions ◽

Lewis Rats ◽

Lymph Node Cells ◽

Lymphocyte Transfer ◽

Spleen And Lymph Nodes

The precise origin of cells infiltrating tuberculin skin reactions was studied with the technique of immunofluorescence. Thymectomized, irradiated Lewis rats were restored with bone marrow from allogeneic or F1 donors. They were passively sensitized to tuberculin by a subsequent transfer of Lewis lymph node cells and were given intradermal skin tests with tuberculoprotein. In 24 hr reactions the majority of cells were shown to be derived from the infused marrow. These results were the same regardless whether the lymphocyte transfer was performed on the day of irradiation and marrow injection or 7 days later. The cells in the tuberculin reactions, marrow, spleen, and lymph nodes not derived from the bone marrow were found to originate in the transferred lymph node cells. The relative percentages of marrow-derived and lymph node-derived cells in the tuberculin reactions remained the same during the 9–24 hr period following skin test.

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