The reactions of swede (Brassica napus) to infection by turnip mosaic virus

Dominant resistance genes identified in Brassica napus lines are effective against some, but not all, Turnip mosaic virus (TuMV) isolates. An infectious clone of an isolate (UK 1) was used as the basis of chimeric virus constructions using resistance-breaking mutants and other isolates to identify the virulence determinants for three dominant resistance genes. For the resistance gene TuRB01, the presence of either of two mutations affecting the cylindrical inclusion (CI) protein converted the avirulent UK 1 to a virulent isolate. Acquisition of such mutations had a slight cost to viral fitness in plants lacking the resistance gene. A similar strategy is being used to identify the virulence determinants for two more resistance genes present in another B. napus line.

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Genetic control of immunity to turnip mosaic virus in winter oilseed rape (Brassica napus ssp. oleifera) and the effect of foreign isolates of the virus

Annals of Applied Biology ◽

10.1111/j.1744-7348.1989.tb06815.x ◽

1989 ◽

Vol 115 (1) ◽

pp. 89-99 ◽

Cited By ~ 41

Author(s):

J. A. WALSH

Keyword(s):

Brassica Napus ◽

Mosaic Virus ◽

Genetic Control ◽

Oilseed Rape ◽

Turnip Mosaic Virus ◽

Winter Oilseed Rape

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Characterisation of resistance to turnip mosaic virus in oilseed rape (Brassica napus) and genetic mapping of TuRB01

Theoretical and Applied Genetics ◽

10.1007/s001220051319 ◽

1999 ◽

Vol 99 (7-8) ◽

pp. 1149-1154 ◽

Cited By ~ 62

Author(s):

J. A. Walsh ◽

A. G. Sharpe ◽

C. E. Jenner ◽

D. J. Lydiate

Keyword(s):

Brassica Napus ◽

Genetic Mapping ◽

Mosaic Virus ◽

Oilseed Rape ◽

Turnip Mosaic Virus

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Turnip mosaic virus: levantamento, identificação, caracterização biológica e molecular e aspectos epidemiológicos dos isolados brasileiros que infectam brássicas

10.31368/pgsaa.2019t.lr002 ◽

2019 ◽

Author(s):

◽

Leilane Karam Rodrigues

Keyword(s):

Brassica Napus ◽

Mosaic Virus ◽

Turnip Mosaic Virus ◽

Em 12

O turnip mosaic virus (TuMV) infecta espécies de diferentes famílias botânicas, sendo o único potyvírus capaz de infectar brássicas. Pode ser transmistido mecanicamente e de modo não persistente por mais de 80 espécies de afídeos. O TuMV é classificado em 12 patotipos, de acordo com reações em linhagens de Brassica napus que contêm (ou não) diferentes genes de resistência ao TuMV. Apesar de ser considerado o vírus mais importante que infecta brássicas no mundo, ainda há pouca informação sobre esse vírus na América do Sul. Com o objetivo de avançar no conhecimento da ocorrência, variabilidade genética e de aspectos biológicos e epidemiológicos do TuMV no Brasil, foram identificados e caracterizados 44 isolados de diferentes regiões produtoras de olerícolas. Cinco isolados tiveram seus genomas completamente sequenciados e, de acordo com análises filogenéticas da porção genômica correspondente à capa proteica, agruparam-se nos clados “World-B”, “Basal-B” e “BR”.

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THE DEVELOPMENT OF Turnip mosaic virus RESISTANT DOUBLED HAPLOID LINES OF Brassica napus L.

Sel skokhozyaistvennaya Biologiya ◽

10.15389/agrobiology.2013.5.122eng ◽

2013 ◽

pp. 122-125

Author(s):

I.А. Zubareva ◽

◽

E.N. Goloveshkina ◽

S.V. Vinogradova ◽

T.N. Gribova ◽

...

Keyword(s):

Brassica Napus ◽

Mosaic Virus ◽

Doubled Haploid ◽

Turnip Mosaic Virus ◽

Brassica Napus L ◽

Doubled Haploid Lines

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Construction of Full-length Infectious cDNA Clones of Two Korean Isolates of Turnip Mosaic Virus Breaking Resistance in Brassica Napus

10.21203/rs.3.rs-803814/v1 ◽

2021 ◽

Author(s):

Zheng-Xing Song ◽

Eun-Young Seo ◽

Wen-Xing Hu ◽

Jong-Hyeon Jeong ◽

Jae Sun Moon ◽

...

Keyword(s):

Brassica Napus ◽

South Korea ◽

Mosaic Virus ◽

Nicotiana Benthamiana ◽

Turnip Mosaic Virus ◽

Cdna Clones ◽

Infectious Clones ◽

The World ◽

Complete Genomes ◽

Systemic Symptoms

Abstract In this work, two new Turnip mosaic virus (TuMV) strains (Canola-12 and Canola-14) overcoming resistance in canola (Brassica napus) were isolated from a B. napus sample which showed typical TuMV-like symptoms and was collected from Gimcheon city, South Korea in 2020. Complete genomes and infectious clones of each isolate were obtained. Phylogenetic analysis indicated that the strains isolated from canola belonged to the World-B group. Both infectious clones which were driven by 35S and T7 promoters induced systemic symptoms on Nicotiana benthamiana and B. napus. To our knowledge, this is the first report of TuMV infecting B. napus in South Korea.

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Evaluation of resistance to Turnip mosaic virus in Australian Brassica napus genotypes

Australian Journal of Agricultural Research ◽

10.1071/ar06096 ◽

2007 ◽

Vol 58 (1) ◽

pp. 67 ◽

Cited By ~ 12

Author(s):

Brenda A. Coutts ◽

John A. Walsh ◽

Roger A. C. Jones

Keyword(s):

Brassica Napus ◽

Mosaic Virus ◽

Resistance Genes ◽

Systemic Infection ◽

Turnip Mosaic Virus ◽

Resistance Response ◽

Breeding Lines ◽

Necrotic Spots ◽

Low Incidence ◽

Tumv Resistance

Forty-three Australian cultivars or breeding lines of Brassica napus (canola, oilseed rape) and 2 cultivars of Brassica juncea (mustard) were inoculated with infective sap containing isolate WA-Ap of Turnip mosaic virus (TuMV), which belongs to TuMV pathotype 8. The types of reactions obtained were: necrotic spots in inoculated leaves without systemic infection (RN), chlorotic blotches in inoculated leaves without systemic infection (R), and chlorotic blotches in inoculated leaves accompanied by systemic infection that consisted of either necrotic spots (+N) or chlorotic blotches (+). The RN and +N reactions are consistent with those expected in the presence of 4 strain-specific TuMV resistance genes TuRB01 (+N response), TuRB03 (+N response) and TuRB04 with TuRB05 (RN), with + indicating a susceptible response. However, which resistance gene corresponds to the R response is unclear. The RN (TuRB04 with TuRB05) type of response was the commonest. Only one genotype lacked any TuMV resistance, and segregation for more than one different type of resistance response occurred within 22 genotypes and some segregated for resistance and susceptibility. Some genotypes segregated for all 3 types of resistance response found. The reaction of 2 plants of cv. Rivette was atypical as they developed both necrotic spots in inoculated leaves and systemic chlorotic spots. Since breeding for TuMV resistance is not undertaken in Australia, these results indicate frequent but inadvertent crossing with parental lines carrying TuMV resistance. Widespread occurrence of TuMV resistance genes and the possibility that many Australian TuMV isolates may not be well adapted to B. napus may explain the low incidence of this virus found in Australian B. napus crops.

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Mutations in Turnip mosaic virus P3 and Cylindrical Inclusion Proteins Are Separately Required to Overcome Two Brassica napus Resistance Genes

Virology ◽

10.1006/viro.2002.1519 ◽

2002 ◽

Vol 300 (1) ◽

pp. 50-59 ◽

Cited By ~ 99

Author(s):

Carol E. Jenner ◽

Kenta Tomimura ◽

Kazusato Ohshima ◽

Sara L. Hughes ◽

John A. Walsh

Keyword(s):

Brassica Napus ◽

Mosaic Virus ◽

Resistance Genes ◽

Turnip Mosaic Virus ◽

Cylindrical Inclusion

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Biological and Molecular Properties of a Turnip mosaic virus (TuMV) Strain that Breaks TuMV Resistances in Brassica napus

Plant Disease ◽

10.1094/pdis-08-16-1129-re ◽

2017 ◽

Vol 101 (5) ◽

pp. 674-683 ◽

Cited By ~ 10

Author(s):

Marine G. L. Guerret ◽

Eviness P. Nyalugwe ◽

Solomon Maina ◽

Martin J. Barbetti ◽

Joop A. G. van Leur ◽

...

Keyword(s):

Brassica Napus ◽

Mosaic Virus ◽

Turnip Mosaic Virus ◽

The Other ◽

Australian Isolate ◽

Breeding Line ◽

Breaking Strain ◽

Resistance Breaking ◽

Complete Genomes ◽

Tumv Resistance

A new resistance-breaking strain of Turnip mosaic virus (TuMV) overcomes TuMV resistance genes that currently suppress spread of this virus in Brassica napus crops in the Liverpool Plains region of eastern Australia. Isolates 12.1 and 12.5 of this strain and three other isolates in TuMV pathotypes 1 (NSW-2), 7 (NSW-1), and 8 (WA-Ap1) were inoculated to plants of 19 B. napus cultivars and one breeding line. All plants of these cultivars and the breeding line proved susceptible to 12.1 and 12.5 but developed only resistance phenotypes with WA-Ap1 or mostly resistance phenotypes with NSW-1 and NSW-2. Five different TuMV resistance phenotypes occurred either alone or segregating in different combinations. When these five isolates were inoculated to plants of nine other crop or wild Brassicaceae spp. and four indicator hosts in other families, 12.1 and 12.5 resembled the other three in inducing TuMV resistance phenotypes in some Brassicaceae spp. but not others, and by inducing extreme resistance phenotypes in all inoculated plants of B. oleracea var. botrytis and Raphanus sativus. Therefore, the overall resistance-breaking properties of 12.1 and 12.5 were restricted to B. napus. When isolates 12.1, 12.5, and WA-Ap1 and additional Australian isolate WA-EP1 were sequenced and complete genomes of each compared, 12.1 and 12.5 grouped separately from the other 2 and from all 23 Australian isolates with complete genomes sequenced previously. In addition, there was evidence for at least six separate TuMV introductions to Australia. Spread of this B. napus resistance-breaking strain poses a significant threat to the B. napus oilseed industry. Breeding B. napus cultivars with resistance to this strain constitutes a critical priority for B. napus breeding programs in Australia and elsewhere.

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