Evaluation of resistance to Turnip mosaic virus in Australian Brassica napus genotypes

2007 ◽  
Vol 58 (1) ◽  
pp. 67 ◽  
Author(s):  
Brenda A. Coutts ◽  
John A. Walsh ◽  
Roger A. C. Jones
Keyword(s):  
Brassica Napus ◽  
Mosaic Virus ◽  
Resistance Genes ◽  
Systemic Infection ◽  
Turnip Mosaic Virus ◽  
Resistance Response ◽  
Breeding Lines ◽  
Necrotic Spots ◽  
Low Incidence ◽  
Tumv Resistance

Forty-three Australian cultivars or breeding lines of Brassica napus (canola, oilseed rape) and 2 cultivars of Brassica juncea (mustard) were inoculated with infective sap containing isolate WA-Ap of Turnip mosaic virus (TuMV), which belongs to TuMV pathotype 8. The types of reactions obtained were: necrotic spots in inoculated leaves without systemic infection (RN), chlorotic blotches in inoculated leaves without systemic infection (R), and chlorotic blotches in inoculated leaves accompanied by systemic infection that consisted of either necrotic spots (+N) or chlorotic blotches (+). The RN and +N reactions are consistent with those expected in the presence of 4 strain-specific TuMV resistance genes TuRB01 (+N response), TuRB03 (+N response) and TuRB04 with TuRB05 (RN), with + indicating a susceptible response. However, which resistance gene corresponds to the R response is unclear. The RN (TuRB04 with TuRB05) type of response was the commonest. Only one genotype lacked any TuMV resistance, and segregation for more than one different type of resistance response occurred within 22 genotypes and some segregated for resistance and susceptibility. Some genotypes segregated for all 3 types of resistance response found. The reaction of 2 plants of cv. Rivette was atypical as they developed both necrotic spots in inoculated leaves and systemic chlorotic spots. Since breeding for TuMV resistance is not undertaken in Australia, these results indicate frequent but inadvertent crossing with parental lines carrying TuMV resistance. Widespread occurrence of TuMV resistance genes and the possibility that many Australian TuMV isolates may not be well adapted to B. napus may explain the low incidence of this virus found in Australian B. napus crops.

scholarly journals Resistance Phenotypes in Diverse Accessions, Breeding Lines, and Cultivars of Three Mustard Species Inoculated with Turnip mosaic virus

Plant Disease ◽  
2010 ◽  
Vol 94 (11) ◽  
pp. 1290-1298 ◽  
Author(s):  
Monica A. Kehoe ◽  
Brenda A. Coutts ◽  
Roger A. C. Jones
Keyword(s):  
Mosaic Virus ◽  
Resistance Genes ◽  
Indian Mustard ◽  
Systemic Infection ◽  
Turnip Mosaic Virus ◽  
Biofuel Production ◽  
Systemic Necrosis ◽  
Breeding Lines ◽  
B Genome ◽  
A Genome

The responses of 44 accessions, breeding lines, or cultivars of Brassica juncea (Indian mustard), 9 of B. carinata (Ethiopian mustard), 5 of B. nigra (black mustard), and 6 crosses between B. juncea and B. napus (canola) to sap inoculation with Turnip mosaic virus (TuMV) were investigated. Eleven different phenotypes were obtained, including six previously recognized in B. napus (+, O, R, RN, RN/+, and +N) and five not recorded before (+St, RN/St, RN/St/+, +N1, and +ND). All but two (+ and +St) were resistance phenotypes. The resistance phenotypes in B. carinata and B. juncea × B. napus crosses prevented systemic infection but those in B. juncea and B. nigra included systemic necrosis. Absence of systemic invasion associated with resistance phenotypes in B. carinata was confirmed by graft inoculations. The resistance phenotypes may reflect the presence of known TuMV resistance genes located in the A genome or unknown genes in the B genome in B. juncea, unknown resistance genes in the B or C genomes in B. carinata, and unknown resistance genes in the B genome in B. nigra. Further research to identify the resistance genes involved would establish the potential usefulness of these resistance phenotypes in breeding TuMV-resistant mustard cultivars for biofuel production.

scholarly journals Turnip mosaic virus determinants of virulence for Brassica napus resistance genes

2002 ◽  
Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽  
pp. S155-S157
Author(s):  
C.E. Jenner ◽  
F. Sánchez ◽  
K. Tomimura ◽  
K. Ohshima ◽  
F. Ponz ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Infectious Clone ◽  
Turnip Mosaic Virus ◽  
Viral Fitness ◽  
Virulence Determinants ◽  
Similar Strategy ◽  
Napus Line

Dominant resistance genes identified in Brassica napus lines are effective against some, but not all, Turnip mosaic virus<br />(TuMV) isolates. An infectious clone of an isolate (UK 1) was used as the basis of chimeric virus constructions using<br />resistance-breaking mutants and other isolates to identify the virulence determinants for three dominant resistance genes.<br />For the resistance gene TuRB01, the presence of either of two mutations affecting the cylindrical inclusion (CI) protein<br />converted the avirulent UK 1 to a virulent isolate. Acquisition of such mutations had a slight cost to viral fitness in<br />plants lacking the resistance gene. A similar strategy is being used to identify the virulence determinants for two more<br />resistance genes present in another B. napus line.

scholarly journals Mutations in Turnip mosaic virus P3 and Cylindrical Inclusion Proteins Are Separately Required to Overcome Two Brassica napus Resistance Genes

Virology ◽  
2002 ◽  
Vol 300 (1) ◽  
pp. 50-59 ◽  
Author(s):  
Carol E. Jenner ◽  
Kenta Tomimura ◽  
Kazusato Ohshima ◽  
Sara L. Hughes ◽  
John A. Walsh
Keyword(s):  
Brassica Napus ◽  
Mosaic Virus ◽  
Resistance Genes ◽  
Turnip Mosaic Virus ◽  
Cylindrical Inclusion

scholarly journals Biological and Molecular Properties of a Turnip mosaic virus (TuMV) Strain that Breaks TuMV Resistances in Brassica napus

Plant Disease ◽  
2017 ◽  
Vol 101 (5) ◽  
pp. 674-683 ◽  
Author(s):  
Marine G. L. Guerret ◽  
Eviness P. Nyalugwe ◽  
Solomon Maina ◽  
Martin J. Barbetti ◽  
Joop A. G. van Leur ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Mosaic Virus ◽  
Turnip Mosaic Virus ◽  
The Other ◽  
Australian Isolate ◽  
Breeding Line ◽  
Breaking Strain ◽  
Resistance Breaking ◽  
Complete Genomes ◽  
Tumv Resistance

A new resistance-breaking strain of Turnip mosaic virus (TuMV) overcomes TuMV resistance genes that currently suppress spread of this virus in Brassica napus crops in the Liverpool Plains region of eastern Australia. Isolates 12.1 and 12.5 of this strain and three other isolates in TuMV pathotypes 1 (NSW-2), 7 (NSW-1), and 8 (WA-Ap1) were inoculated to plants of 19 B. napus cultivars and one breeding line. All plants of these cultivars and the breeding line proved susceptible to 12.1 and 12.5 but developed only resistance phenotypes with WA-Ap1 or mostly resistance phenotypes with NSW-1 and NSW-2. Five different TuMV resistance phenotypes occurred either alone or segregating in different combinations. When these five isolates were inoculated to plants of nine other crop or wild Brassicaceae spp. and four indicator hosts in other families, 12.1 and 12.5 resembled the other three in inducing TuMV resistance phenotypes in some Brassicaceae spp. but not others, and by inducing extreme resistance phenotypes in all inoculated plants of B. oleracea var. botrytis and Raphanus sativus. Therefore, the overall resistance-breaking properties of 12.1 and 12.5 were restricted to B. napus. When isolates 12.1, 12.5, and WA-Ap1 and additional Australian isolate WA-EP1 were sequenced and complete genomes of each compared, 12.1 and 12.5 grouped separately from the other 2 and from all 23 Australian isolates with complete genomes sequenced previously. In addition, there was evidence for at least six separate TuMV introductions to Australia. Spread of this B. napus resistance-breaking strain poses a significant threat to the B. napus oilseed industry. Breeding B. napus cultivars with resistance to this strain constitutes a critical priority for B. napus breeding programs in Australia and elsewhere.

Genetic mapping of the novel Turnip mosaic virus resistance gene TuRB03 in Brassica napus

2003 ◽  
Vol 107 (7) ◽  
pp. 1169-1173 ◽  
Author(s):  
S. L. Hughes ◽  
P. J. Hunter ◽  
A. G. Sharpe ◽  
M. J. Kearsey ◽  
D. J. Lydiate ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Mapping ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Virus Resistance ◽  
Turnip Mosaic Virus ◽  
The Novel ◽  
Virus Resistance Gene

scholarly journals The Dual Role of the Potyvirus P3 Protein of Turnip mosaic virus as a Symptom and Avirulence Determinant in Brassicas

2003 ◽  
Vol 16 (9) ◽  
pp. 777-784 ◽  
Author(s):  
Carol E. Jenner ◽  
Xiaowu Wang ◽  
Kenta Tomimura ◽  
Kazusato Ohshima ◽  
Fernando Ponz ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Genes ◽  
Plant Virus ◽  
Turnip Mosaic Virus ◽  
Single Amino Acid ◽  
Infection Cycle ◽  
In Planta ◽  
Napus Line ◽  
The Uk

Two isolates of the potyvirus Turnip mosaic virus (TuMV), UK 1 and CDN 1, differ both in their general symptoms on the susceptible propagation host Brassica juncea and in their ability to infect B. napus lines possessing a variety of dominant resistance genes. The isolate CDN 1 produces a more extreme mosaic in infected brassica leaves than UK 1 and is able to overcome the resistance genes TuRB01, TuRB04, and TuRB05. The resistance gene TuRB03, in the B. napus line 22S, is effective against CDN 1 but not UK 1. The nucleic acid sequences of the UK 1 and CDN 1 isolates were 90% identical. The C-terminal half of the P3 protein was identified as being responsible for the differences in symptoms in B. juncea. A single amino acid in the P3 protein was found to be the avirulence determinant for TuRB03. Previous work already has identified the P3 as an avirulence determinant for TuRB04. Our results increase the understanding of the basis of plant-virus recognition, show the importance of the potyviral P3 gene as a symptom determinant, and provide a role in planta for the poorly understood P3 protein in a normal infection cycle.

scholarly journals Involvement of Beet western yellows virus, Cauliflower mosaic virus, and Turnip mosaic virus in Internal Disorders of Stored White Cabbage

2002 ◽  
Vol 92 (8) ◽  
pp. 816-826 ◽  
Author(s):  
P. J. Hunter ◽  
J. E. Jones ◽  
J. A. Walsh
Keyword(s):  
Mosaic Virus ◽  
Leaf Tissue ◽  
Subsequent Development ◽  
Turnip Mosaic Virus ◽  
Cauliflower Mosaic Virus ◽  
Beet Western Yellows Virus ◽  
Growing Seasons ◽  
White Cabbage ◽  
Necrotic Spots ◽  
Internal Necrosis

Experiments over two growing seasons clearly showed that Turnip mosaic virus (TuMV) infection was associated with internal necrosis (sunken necrotic spots 5 to 10 mm in diameter) and Beet western yellows virus (BWYV) infection was associated with collapse of leaf tissue at the margins (tipburn) in heads of stored white cabbage (Brassica oleracea var. capitata). Virtually no tipburn was seen in cv. Polinius, whereas cv. Impala was affected severely. Internal necrotic spots were seen in both cultivars. BWYV appeared to interact with TuMV. Plants infected with both viruses showed a lower incidence of external symptoms and had less internal necrosis than plants infected with TuMV alone. Cauliflower mosaic virus (CaMV) did not induce significant amounts of internal necrosis or tipburn, but did, in most cases, exacerbate symptoms caused by TuMV and BWYV. BWYV-induced tipburn worsened significantly during storage. Post-transplanting inoculation with TuMV induced more internal necrosis than pre-transplant inoculation. There was a significant association between detection of TuMV just prior to harvest and subsequent development of internal necrotic spots. Individually, all three viruses significantly reduced the yield of cv. Polinius, whereas only BWYV and CaMV treatments reduced the yield of cv. Impala.

Characterisation of resistance to turnip mosaic virus in oilseed rape (Brassica napus) and genetic mapping of TuRB01

1999 ◽  
Vol 99 (7-8) ◽  
pp. 1149-1154 ◽  
Author(s):  
J. A. Walsh ◽  
A. G. Sharpe ◽  
C. E. Jenner ◽  
D. J. Lydiate
Keyword(s):  
Brassica Napus ◽  
Genetic Mapping ◽  
Mosaic Virus ◽  
Oilseed Rape ◽  
Turnip Mosaic Virus
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