Transfer of resistance to potato leafroll virus, potato virus Y and potato virus X from Solarium brevidens to S. tuberosum through symmetric and designed asymmetric somatic hybridisation

O vírus do enrolamento da folha da batata (Potato leafroll virus, PLRV) e o vírus Y da batata (Potato virus Y, PVY) constituem as principais causas da degenerescência da batata-semente no Brasil. Com o objetivo de determinar, nas condições do Rio Grande do Sul, a resistência de campo de genótipos de batata à infecção por estes vírus, avaliaram-se, na presença de infectores, durante três plantios consecutivos de primavera, 20 cultivares e clones de batata. A detecção dos vírus foi efetuada por meio de testes sorológicos (DAS-ELISA). Pela análise de agrupamento os genótipos foram separados em três grupos para resistência ao PLRV (Elvira, Achat, Bintje, Monalisa, Monte Bonito, Panda e Araucária, resistentes; Baronesa, Asterix, Atlantic, 2CRI-1149-1-78, C-1226-35-80, Astrid, C-1714-7-94, A-1139-12-92, Macaca, Eliza e Santo Amor, suscetíveis; Catucha e Cristal, muito suscetíveis) e em quatro grupos para resistência ao PVY (Asterix, Astrid, Catucha, Cristal, Macaca, Monte Bonito, A-1139-12-92, C-1226-35-80 e C-1714-7-94, resistentes; Baronesa, Santo Amor, Monalisa, Panda e 2CRI-1149-1-78, resistentes intermediários; Bintje, Atlantic, Elvira e Araucária, suscetíveis; Achat e Eliza, muito suscetíveis).

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Multiple DNA markers for evaluation of resistance against Potato virus Y, Potato virus S and Potato leafroll virus

Czech Journal of Genetics and Plant Breeding ◽

10.17221/180/2016-cjgpb ◽

2018 ◽

Vol 54 (No. 1) ◽

pp. 30-33 ◽

Cited By ~ 2

Author(s):

M. Naderpour ◽

L. Sadeghi

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Simultaneous Detection ◽

Potato Leafroll Virus ◽

Potato Seed ◽

Breeding Programs ◽

Potato Virus S ◽

Sequence Characterized Amplified Region ◽

Leafroll Virus ◽

Resistance To Viruses

Molecular markers within or close to genes of interest play essential roles in marker-assisted selection. PCR-based markers have been developed for numerous traits in different plant species including several genes conferring resistance to viruses in potato. In the present work, rapid and reliable approaches were developed for the simultaneous detection of Ryadg and Ry-fsto, Ns, and PLRV.1 genes conferring resistance to Potato virus Y, Potato virus S and Potato leafroll virus, respectively, on the basis of previously published and newly modified markers. The sequence characterized amplified region (SCAR) markers for Ryadg, Ns and PLRV1 and the newly modified cleaved amplified polymorphic sequences (CAPS) marker for Ry-fsto were amplified in one PCR reaction which could simply characterize Ryadg and PLRV.1 resistance. Additional digestion of amplicons with EcoRV and MfeI for genotyping the Ry-fsto and Ns resistance genes, respectively, was needed. The effectiveness of genotyping in triplex and tetraplex PCRs was tested on 35 potato varieties used for potato seed production and breeding programs.

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Effect of Mixed Viral Infections (Potato Virus Y–Potato Leafroll Virus) on Biology and Preference of Vectors Myzus persicae and Macrosiphum euphorbiae (Hemiptera: Aphididae)

Journal of Economic Entomology ◽

10.1093/jee/100.3.646 ◽

2007 ◽

Vol 100 (3) ◽

pp. 646-655 ◽

Cited By ~ 13

Author(s):

Rajagopalbabu Srinivasan ◽

Juan M. Alvarez

Keyword(s):

Potato Virus ◽

Myzus Persicae ◽

Potato Virus Y ◽

Viral Infections ◽

Potato Leafroll Virus ◽

Macrosiphum Euphorbiae ◽

Leafroll Virus

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Comparative detectability of potato leafroll virus, potato virus X and potato virus S using enzyme-linked immunosorbent assay (ELISA) and serologically specific electron microscopy (SSEM)

American Potato Journal ◽

10.1007/bf02853465 ◽

1987 ◽

Vol 64 (4) ◽

pp. 205-207

Author(s):

A. L. Braun ◽

D. C. Opgenorth

Keyword(s):

Electron Microscopy ◽

Immunosorbent Assay ◽

Potato Virus ◽

Potato Virus X ◽

Potato Leafroll Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Potato Virus S ◽

Leafroll Virus

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Molecular Characterization of Potato leafroll virus, Potato virus A, and Potato virus X Isolates from Potatoes in Alaskan Cities and Villages

Plant Health Progress ◽

10.1094/php-2011-0209-01-br ◽

2011 ◽

Vol 12 (1) ◽

pp. 39 ◽

Cited By ~ 2

Author(s):

Nancy L. Robertson ◽

Jeffrey Smeenk ◽

Jodie M. Anderson

Keyword(s):

Molecular Characterization ◽

Potato Virus ◽

Potato Virus X ◽

Potato Leafroll Virus ◽

First Report ◽

Potato Virus A ◽

Potato Viruses ◽

The World ◽

Leafroll Virus

Although all three viruses are commonly found in potatoes throughout the world, this is the first report of potato viruses from Alaska to be sequenced and molecularly analyzed for comparisons with known viruses. Accepted for publication 17 January 2011. Published 9 February 2011.

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