NUTRITIONAL EVALUATION OF BEAN (PHASEOLUS VULGARIS) PROTEIN. IN VIVO VERSUS IN VITRO PROCEDURE

Abstract Objectives The common dry bean (Phaseolus vulgaris L.) is a globally produced pulse crop and an important source of protein and micronutrients for millions of people across Latin America and Africa. In these regions, energy for cooking is expensive or scarce and long cooking times deter consumers from purchasing beans. In addition, many of the preferred black and red seed types have phytate and polyphenols that limit the absorption of trace minerals. Yellow beans are unique because their seed coats are rich in kaempferol 3-glucoside, a recently discovered promoter of iron absorption. Several market classes of yellow beans are sold throughout Latin America and Africa, where they are marketed at premium prices for their fast cooking tendencies. Exploring the yellow bean's unique heritage to develop new fast cooking varieties that deliver more absorbable iron would be useful for regions where inhabitants have limited access to fuelwood for cooking. This study compared the iron bioavailability of three fast cooking yellow beans from Africa with contrasting seed coat colors (Manteca, Amarillo, Njano) to slower cooking white and red kidney commercial varieties from North America (Table 1). Methods Cooked beans were formulated into diets with the complementary food crops of potato, rice and cabbage. Iron bioavailability was measured as ferritin formation in an in vitro digestion Caco-2 bioassay and the ability to maintain total body iron hemoglobin (Hb-Fe) during a 6 week in vivo (Gallus gallus) feeding trial. Results Animals fed yellow bean diets had faster growth rates, accumulated more dietary iron and had higher Hb-Fe than animals fed either kidney bean diet (Figure 1). In contrast to yellow beans, the kidney beans had almost no kaempferol 3-glucoside (Table 2). When compared to the other four bean based diets, the fast cooking Manteca yellow bean diet had the highest Caco-2 ferritin formation in vitro (Table 3) and delivered the largest increase in Hb-Fe in vivo (Figure 1). Conclusions Through the added benefit of fast preparation times and improved iron quality after cooking, this study provides evidence that the Manteca market class is worthy of germplasm enhancement as a new convenience food to help alleviate trace mineral deficiencies in regions where beans are widely accepted as a dietary staple. Funding Sources USDA-NIFA. Supporting Tables, Images and/or Graphs

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L-Phenylalanine ammonia-lyase from Phaseolus vulgaris: partial degradation of enzyme subunits in vitro and in vivo

Biochimica et Biophysica Acta (BBA) - General Subjects ◽

10.1016/0304-4165(86)90006-1 ◽

1986 ◽

Vol 881 (2) ◽

pp. 210-221 ◽

Cited By ~ 29

Author(s):

G. Paul Bolwell ◽

Jan Sap ◽

Carole L. Cramer ◽

Christopher J. Lamb ◽

Wolfgang Schuch ◽

...

Keyword(s):

Phaseolus Vulgaris ◽

Phenylalanine Ammonia Lyase ◽

Enzyme Subunits ◽

Partial Degradation

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Nutritional Evaluation, in vitro Free Radical Scavenging and in vivo Anti-inflammatory Effects of Gisekia pharnaceoides and Identification of Kaempferol as a Nutraceutical Agent

British Biotechnology Journal ◽

10.9734/bbj/2011/597 ◽

2011 ◽

Vol 1 (3) ◽

pp. 113-135 ◽

Cited By ~ 1

Author(s):

Chinnasammi Gandhimathi

Keyword(s):

Free Radical ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Nutritional Evaluation ◽

Anti Inflammatory

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Ação fungicida do acaricida azocyclotin sobre a antracnose do feijoeiro comum

Bragantia ◽

10.1590/s0006-87052005000200011 ◽

2005 ◽

Vol 64 (2) ◽

pp. 241-248

Author(s):

Ademir Santini ◽

Margarida Fumiko Ito ◽

Jairo Lopes de Castro ◽

Marcio Akira Ito ◽

Juliana Cristina Goto

Keyword(s):

Phaseolus Vulgaris ◽

Colletotrichum Lindemuthianum ◽

Phaseolus Vulgaris L

A cultura do feijoeiro (Phaseolus vulgaris L.) pode ser afetada por muitas doenças e dentre elas destaca-se a antracnose, causada pelo fungo Colletotrichum lindemuthianum. O acaricida azocyclotin (AZ) foi avaliado in vitro, em plântulas e em condições de campo, quanto ao efeito em C. lindemuthianum. Foram avaliados sete tratamentos in vitro: 1) testemunha; 2) AZ-1 mg L-1; 3) Trifenil hidróxido de estanho (THE)-1 mg L-1; 4) AZ-10 mg L-1; 5) THE-10 mg L-1; 6) AZ-100 mg L-1 e 7) THE-100 mg L-1 e 13 tratamentos in vivo: 1) testemunha; 2) AZ aplicado 24 horas antes da inoculação (AZ-24); 3) THE-24; 4) AZ-48; 5) THE-48; 6) AZ-72; 7) THE-72; 8) AZ-96; 9) THE-96; 10) AZ-120; 11) THE-120; 12) AZ-144 e 13) THE-144. Azocyclotin foi avaliado à dose de 125 g i.a.100 L-1 de água e trifenil hidróxido de estanho a 41,25 g i.a.100 L-1. Os delineamentos experimentais foram inteiramente ao acaso, com cinco repetições. Em condições de campo, foi realizado um experimento com seis tratamentos. Os tratamentos e as doses em g ha-1 de i.a foram: 1) tebuconazole + trifenil hidróxido de estanho (100 + 200); 2) tebuconazole + trifloxystrobin (40 + 100); 3) trifloxystrobin (125); 4) tebuconazole + azocyclotin (100 + 500); 5) azocyclotin (500) e 6) testemunha. O delineamento foi em blocos ao acaso, com quatro repetições. Para a avaliação in vitro foram medidos diâmetros ortogonais do crescimento micelial do fungo em BDA; in vivo e no campo usou-se escala de notas de 1 a 9, sendo 1 = sem sintoma e 9 = igual ou mais de 25% de área foliar afetada. In vitro, o tratamento 7 proporcionou maior inibição do desenvolvimento micelial. Em plântulas, observou-se controle de C. lindemuthianum até 144 horas, pelos dois produtos. Uma nova constatação em campo foi o controle de antracnose pelo acaricida azocyclotin, em que se observou também efeito sobre mancha-angular e mancha-de-alternária. Concluiu-se que o acaricida azocyclotin é eficiente no controle da antracnose do feijoeiro, semelhante ao trifenil hidróxido de estanho.

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