SOMATIC EMBRYOGENESIS IN ANISE (PIMPINELLA ANISUM L.): THE EFFECT OF PROLINE ON EMBRYOGENIC CALLUS FORMATION AND ABA ON ADVANCED EMBRYO DEVELOPMENT

1999 ◽  
Vol 23 (1) ◽  
pp. 17-32 ◽  
Author(s):  
JOHN BELA ◽  
KALIDAS SHETTY
Keyword(s):  
Somatic Embryogenesis ◽  
Embryo Development ◽  
Embryogenic Callus ◽  
Callus Formation ◽  
Pimpinella Anisum ◽  
Advanced Embryo ◽  
Embryogenic Callus Formation

scholarly journals The evaluation of java fine flavor cocoa propagation through somatic embryogenesis technique for germplasm preservation

2021 ◽  
Vol 306 ◽  
pp. 01056
Author(s):  
Sulistyani Pancaningtyas
Keyword(s):  
Somatic Embryogenesis ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Basic Structure ◽  
Embryonic Cells ◽  
Regeneration Rate ◽  
Germplasm Preservation ◽  
Embryogenic Callus Formation ◽  
Secondary Somatic Embryos

Somatic embryogenesis is one of the newest technology that applied for the mass production of cocoa. This research aims to evaluate the regeneration rate of somatic embryos through somatic embryogenesis propagation techniques on java fine flavor cocoa. Cultivars in this study are ICCRI 01, ICCRI 02, DR 1, DR 2, DRC 16, DR 38, PNT 16, and PNT 30. Observations include parameters to determine the percentage of primary callus and embryogenic callus formation and the number of somatic embryos produced. Based on data, the ability of callus to produce primary embryos is highly dependent on plant cultivars and explant sources. Five cultivars showed a higher regeneration rate using explants from the petal part, while the rest showed a higher regeneration rate using explants from the staminode section. Embryogenic callus from each cacao cultivar has the same basic structure: a nodular friable structure consisting of many embryonic cells. Some fine flavor cacao cultivars that were able to produce callus and primary somatic embryos could not produce secondary somatic embryos and plantlets. However, two cultivars, which had low potential in producing primary embryos, had the high ability to produce secondary somatic embryos and develop into plantlets.

scholarly journals INDUCTION AND MORPHOLOGICAL CHARACTERISTICS ASSOCIATED WITH EMBRYOGENESIS OF OENANTHE STOLONIFERA DC.

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 693e-693
Author(s):  
Ji-Weon Lee ◽  
Byoung-Yil Lee
Keyword(s):  
Somatic Embryogenesis ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Morphological Characteristics ◽  
Liquid Media ◽  
Friable Embryogenic Callus ◽  
Development And Differentiation ◽  
Rapid Mass ◽  
Embryogenic Callus Formation

The study was carried out to examine the appropriate media, explant sources, and suitable growth regulators for somatic embryogenesis to establish a rapid mass production system via somatic embryogenesis in Oenanthe stolonifera DC. Modified MS media containing higher concentrations of NO3-N were more effective for the formation and development of the somatic embryos from embryogenic callus. Liquid media were more effective for the production of somatic embryos than solidified media. Immature florets were found to be the most competent explant sources for embryogenic callus formation. 2,4-D at 1mg/l was highly effective for the formation of embryogenic callus but inhibitory for the development and differentiation of somatic embryo. Somatic embryos were developed from the translucent and friable embryogenic callus. Addition of BA promoted the callus growth synefgistically with NAA and 2,4-D, but the production of embryogenic callus was inhibited by BA.

scholarly journals Induction of somatic embryogenesis in two cultivars of anthurium analysed by scanning electron microscopy

2019 ◽  
Vol 13 ◽  
pp. 1
Author(s):  
Priscila Bezerra Dos Santos Melo ◽  
Ana Cristina Portugal Pinto de Carvalho ◽  
Cândida Hermínia Campos de Magalhães Bertini ◽  
Celli Rodrigues Muniz ◽  
Adroaldo Guimarães Rossetti
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Somatic Embryogenesis ◽  
Callus Formation ◽  
Nodal Segments ◽  
Embryogenic Calli ◽  
Evaluation Period ◽  
Mean Values ◽  
Embryogenic Callus Formation ◽  
Scanning Electron

Somatic embryogenesis is an advantageous tool in the commercial production of micropropagated anthurium plantlets. As such, the aim of this study was to establish a protocol for the induction of somatic embryogenesis in Jureia and Luau cultivars. Defoliated nodal segments, 1.0 cm in length and containing one bud, were used as explants. The experimental design was completely randomised, in a 2 x 3 x 5 factorial scheme (cultivar: Jureia and Luau x auxin: 2,4-D, NAA and Picloram x concentration: 0, 2.5, 5.0, 7.5, and 10.0 μM), with 30 treatments in a scheme of plots split over time (15, 30, 45, 60, 75 and 90 days). The anatomy and percentage of embryogenic callus formation were analysed. The structures formed, analysed by scanning electron microscopy, corresponded to embryogenic calli. The Luau cultivar was superior in forming embryogenic calli. For the two cultivars, among the auxins under study, NAA demonstrated a greater induction potential for somatic embryogenesis, with the concentration of 7.5 μM giving the highest mean values. The 90-day evaluation period showed the maximum formation of embryogenic calli; however, mean values were fairly similar to the 75-day evaluation period. To induce embryogenic calli, therefore, it is suggested that the nodal segments be inoculated into a culture medium with added NAA growth regulator at a concentration of 7.5 μM, and that the explants remain in this medium for 75 days after inoculation.

scholarly journals Bioproduction of Diosgenin in Callus Cultures of Balanites aegyptiaca: Effect of Growth Regulators, Explants and Somatic Embryogenesis

2006 ◽  
Vol 1 (3) ◽  
pp. 1934578X0600100
Author(s):  
Bishnu P. Chapagain ◽  
Vinod Saharan ◽  
Dan Pelah ◽  
Ram C. Yadav ◽  
Zeev Wiesman
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Basal Medium ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Balanites Aegyptiaca ◽  
Basal Media

This study describes the effects of plant growth regulators, explants, and somatic embryogenesis on in vitro production of the steroidal sapogenin, diosgenin, in callus cultures of the Balanites aegyptiaca (L.) Del.(desert date). Root, shoot, hypocotyl, and epicotyl callus culture of B. aegyptiaca, were raised on MS basal media supplemented with various combinations of either 2,4-D and NAA alone, or with BAP. The diosgenin content (on a dry weight basis) was found to be highest when calli were cultured in MS basal medium supplemented with 1.0 mg l−1 2,4-D alone and/or in combination with 0.5 mg l−1 BAP. However, the callus growth was highest in media supplemented with 2.5 or 3.0 mg l−1 2,4-D. MS basal media supplemented with 2,4-D 2.5 mg l−1 alone and in combination with 0.5 mg l−1 BAP induced pre-embryogenic callus formation on root cultures. When these pre-embryogenic callus cultures were used to establish cell suspension cultures, two growth densities were obtained in embryogenic suspension cultures, inducing clusters of somatic embryos at various stages of development. The maximum number of somatic embryos were obtained at the fifth week on the medium supplemented with 1.0 mg l−1 2,4-D. However, the diosgenin content in these somatic cells was found to be lower compared to the explant calluses. This study revealed that production of diosgenin in callus cultures of B. aegyptiaca is possible, but the amount is significantly affected by the growth regulators, type of explants, and somatic embryogenesis.

Embryogenic callus formation from maize protoplasts

Planta ◽  
1987 ◽  
Vol 172 (2) ◽  
pp. 245-251 ◽  
Author(s):  
K. K. Kamo ◽  
K. L. Chang ◽  
M. E. Lynn ◽  
T. K. Hodges
Keyword(s):  
Embryogenic Callus ◽  
Callus Formation ◽  
Embryogenic Callus Formation
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