CROSS-IMMUNITY TESTS BETWEEN ANAPLASMA CENTRALE (SOUTH AFRICA) AND ANAPLASMA MARGINALE (AUSTRALIA)

Bovine anaplasmosis caused by the intraerythrocytic rickettsial pathogenAnaplasma marginaleis endemic in South Africa.Anaplasma marginalesubspeciescentralealso infects cattle; however, it causes a milder form of anaplasmosis and is used as a live vaccine againstA. marginale. There has been less interest in the epidemiology ofA. marginalesubsp.centrale, and, as a result, there are few reports detecting natural infections of this organism. When detected in cattle, it is often assumed that it is due to vaccination, and in most cases, it is reported as coinfection withA. marginalewithout characterization of the strain. A total of 380 blood samples from wild ruminant species and cattle collected from biobanks, national parks, and other regions of South Africa were used in duplex real-time PCR assays to simultaneously detectA. marginaleandA. marginalesubsp.centrale.PCR results indicated high occurrence ofA. marginalesubsp.centraleinfections, ranging from 25 to 100% in national parks. Samples positive forA. marginalesubsp.centralewere further characterized using themsp1aSgene, a homolog ofmsp1α ofA. marginale, which contains repeats at the 5′ ends that are useful for genotyping strains. A total of 47 Msp1aS repeats were identified, which corresponded to 32A. marginalesubsp.centralegenotypes detected in cattle, buffalo, and wildebeest. RepeatAnalyzer was used to examine strain diversity. Our results demonstrate a diversity ofA. marginalesubsp.centralestrains from cattle and wildlife hosts from South Africa and indicate the utility ofmsp1aSas a genotypic marker forA. marginalesubsp.centralestrain diversity.

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Epidemiology and evolution of the genetic variability of Anaplasma marginale in South Africa

Ticks and Tick-borne Diseases ◽

10.1016/j.ttbdis.2014.04.011 ◽

2014 ◽

Vol 5 (6) ◽

pp. 624-631 ◽

Cited By ~ 28

Author(s):

Awelani M. Mutshembele ◽

Alejandro Cabezas-Cruz ◽

Moses S. Mtshali ◽

Oriel M.M. Thekisoe ◽

Ruth C. Galindo ◽

...

Keyword(s):

South Africa ◽

Genetic Variability ◽

Anaplasma Marginale

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A Study of the Immunological Relationship of Anaplasma Marginale and Anaplasma Centrale

Research in Veterinary Science ◽

10.1016/s0034-5288(18)34606-x ◽

1967 ◽

Vol 8 (4) ◽

pp. 467-471 ◽

Cited By ~ 19

Author(s):

K.L. Kuttler

Keyword(s):

Anaplasma Marginale ◽

Immunological Relationship ◽

Relationship Of ◽

Anaplasma Centrale

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Cepas atenuadas de Babesia bovis e Babesia bigemina e de Anaplasma centrale como imunógenos no controle da tristeza parasitária bovina

Ciência Rural ◽

10.1590/s0103-84782001000500018 ◽

2001 ◽

Vol 31 (5) ◽

pp. 849-855 ◽

Cited By ~ 2

Author(s):

Ana Maria Sastre Sacco ◽

Raul Henrique Kessler ◽

Cláudio Roberto Madruga

Keyword(s):

Anaplasma Marginale ◽

Babesia Bovis ◽

Babesia Bigemina ◽

Anaplasma Centrale

Neste trabalho foram avaliadas cepas atenuadas de Babesia bovis e B. bigemina e Anaplasma centrale como imunógenos a serem utilizados no controle da Tristeza Parasitária Bovina. O processo de imunização demonstrou ser inócuo, imunogênico e eficiente, pelo menos no que diz respeito às babesias, pois protegeu os animais vacinados frente ao desafio com cepas heterólogas virulentas a campo, que provocou doença clínica e morte nos animais do grupo controle. O desafio a campo pelo Anaplasma marginale não se mostrou muito virulento ou patogênico, não sendo possível concluir que a imunização com A. centrale proteja os animais contra anaplasmose.

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