An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Anaplasma centrale and Anaplasma marginale

1990 ◽  
Vol 25 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Varda Shkap ◽  
Hanna Bin ◽  
Hanna Ungar-Waron ◽  
E. Pipano
Keyword(s):  
Immunosorbent Assay ◽  
Anaplasma Marginale ◽  
Enzyme Linked Immunosorbent Assay ◽  
Anaplasma Centrale

scholarly journals Detection of Cattle Naturally Infected with Anaplasma marginale in a Region of Endemicity by Nested PCR and a Competitive Enzyme-Linked Immunosorbent Assay Using Recombinant Major Surface Protein 5

1998 ◽  
Vol 36 (3) ◽  
pp. 777-782 ◽  
Author(s):  
Susana Torioni de Echaide ◽  
Donald P. Knowles ◽  
Travis C. McGuire ◽  
Guy H. Palmer ◽  
Carlos E. Suarez ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Immunosorbent Assay ◽  
Nested Pcr ◽  
Surface Protein ◽  
Anaplasma Marginale ◽  
Epidemiological Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Major Surface Protein ◽  
Persistent Infections ◽  
Major Surface

A competitive enzyme-linked immunosorbent assay using recombinant major surface protein 5 (rMSP5-cELISA) of Anaplasma marginale was validated in a naturally infected cattle herd in an area of eastern Oregon where A. marginale is endemic. The true positive and negative A. marginale infection status of 235 randomly selected cattle was determined by using a nested PCR (nPCR) coupled with msp5 sequence analysis and hybridization. Judgment of the reliability of the nPCR and hybridization for detection of persistent infections was based on three observations. First, the nPCR was able to detect as few as 30 infected erythrocytes per ml. Second, the nPCR was able to consistently detect low levels of rickettsemia in seven carrier cattle experimentally infected with A. marginale. Third, msp5sequence analysis showed >95% identity among 30 nPCR amplicons from cattle naturally infected with field strains of A. marginale. The nPCR and hybridization identified 151 infected and 84 uninfected cattle among the 235 animals tested. With a cutoff point of 28%, the rMSP5-cELISA showed a sensitivity of 96% and a specificity of 95%. These results indicate that the rMSP5-cELISA can sensitively and specifically detect cattle with naturally acquired persistent A. marginale infections and suggest that it is an excellent assay for epidemiological studies, eradication programs, and regulation of international cattle movement.

scholarly journals Enzyme-linked immunosorbent assay for detection of antibodies to Anaplasma centrale.

1988 ◽  
Vol 50 (4) ◽  
pp. 933-935 ◽  
Author(s):  
Yoshio NAKAMURA ◽  
Shinya SHIMIZU ◽  
Tetsuro MINAMI ◽  
Shingo ITO
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Anaplasma Centrale

scholarly journals Séroprévalence et facteurs de risque des hémoparasitoses (theilériose, babésiose et anaplasmose) chez les bovins dans quatre grandes régions d’élevage du Maroc

2015 ◽  
Vol 67 (4) ◽  
pp. 235 ◽  
Author(s):  
T. Rahali ◽  
H. Sahibi ◽  
A. Sadak ◽  
S. Ait Hamou ◽  
B. Losson ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Facteurs De Risque ◽  
Anaplasma Marginale ◽  
Theileria Annulata ◽  
Enzyme Linked Immunosorbent Assay ◽  
Babesia Bigemina ◽  
Infections Mixtes

Les hémoparasitoses (theilériose, babésiose et anaplasmose) sont des maladies vectorielles graves dont l’impact économique et sur la santé des élevages bovins au Maroc est considérable. L’objectif de cette étude, réalisée dans quatre régions d’élevage bovin laitier, a été de déterminer la séroprévalence et les facteurs de risque de ces infections. Au total 1 040 bovins ont été examinés dans 96 exploitations. La technique competitive enzyme-linked immunosorbent assay (cELISA) et la réaction d’immunofluorescence indirecte ont été utilisées pour la détermination de la séroprévalence, et le test statistique V de Cramer a permis d’analyser les facteurs de risque d’infection des bovins par les hémoparasites. Sur 1 040 sérums analysés, 689 (66,3 p. 100) ont été séropositifs ; la séroprévalence a varié selon le parasite considéré : Theileria annulata (35,4 p. 100), Anaplasma marginale (20,5 p. 100), Babesia bigemina (13,6 p. 100) et B. bovis (12,0 p. 100). Par ailleurs, 155 sérums (14,9 p. 100) ont présenté des infections mixtes (deux ou trois hémoparasites simultané- ment). L’analyse statistique a montré que l’âge (C = 0,62) et le type d’élevage (C = 0,60) ont eu une forte influence sur la séroprévalence, tandis que la race (C = 0,46) et le sexe (C = 0,29) ont eu une influence moindre. Le climat (C = 0,06) n’a pas semblé avoir d’impact. Ces résultats aideront à comprendre la situation épizootique des hémoparasitoses vectorielles chez les bovins afin d’établir une stratégie adéquate pour leur contrôle.

scholarly journals Evaluation of an enzyme-linked immunosorbent assay to detect antibodies against Anaplasma marginale

2000 ◽  
Vol 20 (3) ◽  
pp. 109-112 ◽  
Author(s):  
Cláudio R. Madruga ◽  
Ana Paula C. Marques ◽  
Cássia Rejane B. Leal ◽  
Cristiano M.E. Carvalho ◽  
Flábio R. Araújo ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Fluorescent Antibody ◽  
High Sensitivity ◽  
Antibody Test ◽  
Anaplasma Marginale ◽  
Babesia Bovis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Soluble Antigen ◽  
Babesia Bigemina ◽  
Nitrophenyl Phosphate

A rapid indirect enzyme-linked immunosorbent assay (ELISA) was developed for measuring antibodies against Anaplasma marginale using a partially soluble antigen prepared from semi-purified initial bodies from erythrocytes with 80.0% of rickettsiaemia. This technique utilized alkaline phosphatase and p-nitrophenyl phosphate as reaction indicators. The high sensitivity (100.0%) was confirmed with sera from 100 calves experimentally-infected with A. marginale. All of these animals showed seroconversion before or at the same time of the first rickettsiaemia or even when it was not detected. Also the elevated specificity (94.0%) was confirmed by the low percentage of cross-reactions with sera from animals experimentally-infected with Babesia bigemina and Babesia bovis (1.4 and 6.6%, respectively). Performances of ELISA and indirect fluorescent antibody test (IFAT) with 324 sera from enzootically stable area did not show statistical difference (P>0.05), since the former showed 96.9% and the latter 97.2% of positive reactions. The advantage of this ELISA is a shorter execution time than others developed until now, allowing more samples to be analyzed.

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