Preclinical evidence that the
            PPAR
            γ modulator,
            N
            ‐Acetyl‐
            GED
            ‐0507‐34‐Levo, may protect human hair follicle epithelial stem cells against lichen planopilaris‐associated damage

AbstractThe challenge arises among researchers when hair follicle stem cells (HFSCs) derived from a human hair follicle remain poorly expanded in defined culture medium. In this study, we isolated the HFSCs and they became confluent after 10 days of cultivation. Comparing the viability of HFSCs cultured in defined keratinocytes serum free medium (KSFM) in a coated plate and CnT07 medium in an uncoated plate, the number of HFSCs cultured in CnT07 was significantly higher at days 2, 4, 6 and 8 (P=0.004). The population doubling time of HFSCs was 21.48±0.44 hours in non-coated plates with CnT07 and 30.73±0.75 hours in coated plates with KSFM. Our primary HFSC cultures were positive for CD200 and K15 with brownish color. Flow cytometry analysis showed that the percentage of HFSCs expressing CD200 and K15 were 65.20±3.16 and 72.07±6.62 respectively. After reaching 100% confluence, the HFSCs were differentiated into an epidermal layer in vitro using CnT02-3D defined media. HFSCs were differentiated into an epidermal layer after 2 weeks of induction. Involucrin- and K6-positive cells were detected in the differentiated epidermal layer. This method is a simple technique for HFSC isolation and has a lower cost of processing and labor, and it represents a promising tool for skin tissue engineering.

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The C8/144B monoclonal antibody recognizes cytokeratin 15 and defines the location of human hair follicle stem cells

Journal of Cell Science ◽

10.1242/jcs.111.21.3179 ◽

1998 ◽

Vol 111 (21) ◽

pp. 3179-3188 ◽

Cited By ~ 11

Author(s):

S. Lyle ◽

M. Christofidou-Solomidou ◽

Y. Liu ◽

D.E. Elder ◽

S. Albelda ◽

...

Keyword(s):

Stem Cells ◽

Monoclonal Antibody ◽

Hair Follicle ◽

Human Hair ◽

Expression Cloning ◽

Cell Phenotype ◽

Epithelial Stem Cells ◽

Human Hair Follicle ◽

Hair Follicle Stem Cells ◽

Follicle Stem Cells

Stem cells are vital for the homeostasis of self-renewing tissues such as the hair follicle. Epithelial stem cells have been implicated in tumorigenesis and wound healing, and their manipulation may have wide ranging applications including gene therapy and tissue transplantation. Rodent hair follicle stem cells have been localized to an area of the follicle called the bulge, however, the identification and characterization of human hair follicle stem cells has been hampered by a lack of cellular markers for this area. We have determined that the C8/144B monoclonal antibody, originally generated against a short intracytoplasmic peptide of CD8, preferentially immunostains hair follicle bulge keratinocytes without staining the remaining hair follicle. Using expression cloning, we identified cytokeratin 15 as the keratinocyte protein recognized by the C8/144B monoclonal antibody. By delineating the bulge using this antibody, we demonstrated that bulge cells possess a stem cell phenotype characterized by their slowly-cycling nature, preferential proliferation at the onset of new hair follicle growth, high level of beta1 integrin expression, and expression of cytokeratin 19.

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