Development of an Isavirus minigenome system to study the function of the pocket RNA‐binding domain of the viral nucleoprotein (NP) in salmon cells

2019 ◽  
Vol 43 (2) ◽  
pp. 197-206
Author(s):  
Daniela Toro‐Ascuy ◽  
Alvaro Santibañez ◽  
Victor Peña ◽  
Carolina Beltran‐Pavez ◽  
Luis Cottet ◽  
...  
Keyword(s):  
Rna Binding ◽  
Binding Domain ◽  
Rna Binding Domain ◽  
Minigenome System

scholarly journals Requirement for C-terminal Extension to the RNA Binding Domain for Efficient RNA Binding by Ribosomal Protein L2

2002 ◽  
Vol 66 (3) ◽  
pp. 682-684 ◽  
Author(s):  
Takeshi HAYASHI ◽  
Maino TAHARA ◽  
Kenta IWASAKI ◽  
Yoshiaki KOUZUMA ◽  
Makoto KIMURA
Keyword(s):  
Ribosomal Protein ◽  
Rna Binding ◽  
Binding Domain ◽  
Ribosomal Protein L2 ◽  
Rna Binding Domain

K160 in the RNA‐binding domain of the orf virus virulence factor OV20.0 is critical for its functions in counteracting host antiviral defense

FEBS Letters ◽  
2021 ◽  
Author(s):  
Guan‐Ru Liao ◽  
Yeu‐Yang Tseng ◽  
Ching‐Yu Tseng ◽  
Ying‐Ping Huang ◽  
Ching‐Hsiu Tsai ◽  
...  
Keyword(s):  
Virulence Factor ◽  
Rna Binding ◽  
Binding Domain ◽  
Orf Virus ◽  
Antiviral Defense ◽  
Rna Binding Domain

scholarly journals The RNA binding domain within the nucleoporin Nup153 associates preferentially with single-stranded RNA

RNA ◽  
2004 ◽  
Vol 10 (1) ◽  
pp. 19-27 ◽  
Author(s):  
J. R. BALL
Keyword(s):  
Rna Binding ◽  
Binding Domain ◽  
Rna Binding Domain

scholarly journals Characterization of an RNA-binding domain in the bacteriophage phi 29 connector.

1993 ◽  
Vol 268 (27) ◽  
pp. 20198-20204
Author(s):  
L.E. Donate ◽  
J.M. Valpuesta ◽  
C Mier ◽  
F Rojo ◽  
J.L. Carrascosa
Keyword(s):  
Rna Binding ◽  
Binding Domain ◽  
Rna Binding Domain

scholarly journals Straightening of bulged RNA by the double-stranded RNA-binding domain from the protein kinase PKR

2000 ◽  
Vol 97 (26) ◽  
pp. 14162-14167 ◽  
Author(s):  
X. Zheng ◽  
P. C. Bevilacqua
Keyword(s):  
Protein Kinase ◽  
Rna Binding ◽  
Binding Domain ◽  
Double Stranded Rna ◽  
Rna Binding Domain

TAR-Independent Activation of HIV-1 Requires the Activation Domain but Not the RNA-Binding Domain of Tat

Virology ◽  
1993 ◽  
Vol 195 (2) ◽  
pp. 780-785 ◽  
Author(s):  
J.Paul Taylor ◽  
Mondira Kundu ◽  
Kamel Khalili
Keyword(s):  
Rna Binding ◽  
Binding Domain ◽  
Activation Domain ◽  
Rna Binding Domain ◽  
Hiv 1

scholarly journals Mapping the RNA-binding domain on the Apple chlorotic leaf spot virus movement protein

2005 ◽  
Vol 86 (1) ◽  
pp. 225-229 ◽  
Author(s):  
Masamichi Isogai ◽  
Nobuyuki Yoshikawa
Keyword(s):  
Leaf Spot ◽  
Rna Binding ◽  
Movement Protein ◽  
Binding Domain ◽  
Sequence Specificity ◽  
Nucleic Acid Binding ◽  
Spot Virus ◽  
Uv Crosslinking ◽  
Chlorotic Leaf ◽  
Rna Binding Domain

The RNA-binding properties of the cell-to-cell movement protein (MP) of Apple chlorotic leaf spot virus were analysed. MP was expressed in Escherichia coli and was used in UV-crosslinking analysis, using a digoxigenin–UTP-labelled RNA probe and gel-retardation analysis. The analyses demonstrated that MP bound cooperatively to single-stranded RNA (ssRNA). When analysed for NaCl dependence of the RNA-binding activity, the majority of the MP could bind ssRNA even in binding buffer with 1 M NaCl. Furthermore, competition binding experiments showed that the MP bound preferentially to ssRNA and single-stranded DNA without sequence specificity. MP deletion mutants were used to identify the RNA-binding domain by UV-crosslinking analysis. Amino acid residues 82–126 and 127–287 potentially contain two independently active, single-stranded nucleic acid-binding domains.

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