Spontaneous, synchronous zinc spikes oscillate with neural excitability and calcium spikes in primary hippocampal neuron culture

2021 ◽  
Author(s):  
Chen Zhang ◽  
Drew Maslar ◽  
Taylor F. Minckley ◽  
Kate D. LeJeune ◽  
Yan Qin
Keyword(s):  
Hippocampal Neuron ◽  
Neuron Culture ◽  
Calcium Spikes ◽  
Neural Excitability ◽  
Primary Hippocampal Neuron ◽  
Hippocampal Neuron Culture

scholarly journals Low-Density Primary Hippocampal Neuron Culture

10.3791/55000 ◽  
2017 ◽  
Author(s):  
Reiko T. Roppongi ◽  
Kevin P. Champagne-Jorgensen ◽  
Tabrez J. Siddiqui
Keyword(s):  
Hippocampal Neuron ◽  
Low Density ◽  
Neuron Culture ◽  
Primary Hippocampal Neuron ◽  
Hippocampal Neuron Culture

Sub-chronic Antipsychotic Drug Administration Reverses the Expression of Neuregulin 1 and ErbB4 in a Cultured MK801-Induced Mouse Primary Hippocampal Neuron or a Neurodevelopmental Schizophrenia Model

2016 ◽  
Vol 41 (8) ◽  
pp. 2049-2064 ◽  
Author(s):  
Cunyan Li ◽  
Yamei Tang ◽  
Jingjing Yang ◽  
Xianghui Zhang ◽  
Yong Liu ◽  
...  
Keyword(s):  
Antipsychotic Drug ◽  
Drug Administration ◽  
Hippocampal Neuron ◽  
Neuregulin 1 ◽  
Primary Hippocampal Neuron

scholarly journals Arfgef1 haploinsufficiency in mice alters neuronal endosome composition and decreases membrane surface postsynaptic GABAA receptors

2019 ◽  
Author(s):  
JiaJie Teoh ◽  
Narayan Subramanian ◽  
Maria Elena Pero ◽  
Francesca Bartolini ◽  
Ariadna Amador ◽  
...  
Keyword(s):  
Cell Body ◽  
Membrane Surface ◽  
Seizure Threshold ◽  
List Type ◽  
Recycling Endosome ◽  
Neuron Culture ◽  
Recycling Endosomes ◽  
Neuronal Inhibition ◽  
Early Endosomes ◽  
Hippocampal Neuron Culture

AbstractARFGEF1 encodes a guanine exchange factor involved in intracellular vesicle trafficking, and is a candidate gene for childhood genetic epilepsies. To model ARFGEF1 haploinsufficiency observed in a recent Lennox Gastaut Syndrome patient, we studied a frameshift mutation (Arfgef1fs) in mice. Arfgef1fs/+ pups exhibit signs of developmental delay, and Arfgef1fs/+ adults have a significantly decreased threshold to induced seizures but do not experience spontaneous seizures. Histologically, the Arfgef1fs/+ brain exhibits a disruption in the apical lining of the dentate gyrus and altered spine morphology of deep layer neurons. In primary hippocampal neuron culture, dendritic surface and synaptic but not total GABAA receptors (GABAAR) are reduced in Arfgef1fs/+ neurons with an accompanying decrease in GABAAR-containing recycling endosomes in cell body. Arfgef1fs/+ neurons also display differences in the relative ratio of Arf6+:Rab11+:TrfR+ recycling endosomes. Although the GABAAR-containing early endosomes in Arfgef1fs/+ neurons are comparable to wildtype, Arfgef1fs/+ neurons show an increase in GABAAR-containing lysosomes in dendrite and cell body. Together, the altered endosome composition and decreased neuronal surface GABAAR results suggests a mechanism whereby impaired neuronal inhibition leads to seizure susceptibility.HighlightsArfgef1fs/+ mice have lower seizure threshold but no spontaneous seizure.Arfgef1fs/+ neurons show reduced dendritic surface GABAAR.Arfgef1fs/+ neurons have decreased GABAAR-containing recycling endosome accompanied with an increase in GABAAR-containing lysosomes.

scholarly journals Developmental and Activity-Dependent miRNA Expression Profiling in Primary Hippocampal Neuron Cultures

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e74907 ◽  
Author(s):  
Myrrhe van Spronsen ◽  
Eljo Y. van Battum ◽  
Marijn Kuijpers ◽  
Vamshidhar R. Vangoor ◽  
M. Liset Rietman ◽  
...  
Keyword(s):  
Mirna Expression ◽  
Expression Profiling ◽  
Hippocampal Neuron ◽  
Primary Hippocampal Neuron ◽  
Mirna Expression Profiling ◽  
Activity Dependent
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