Influence of Zinc and Manganese Nanoparticles on Selected Parameters of Turkey Spermatozoa Stored in a Liquid State at 4 °C

The aim of this study was to determine the effect of semen extender supplementation with 25 or 50 μM of zinc nanoparticles (ZnNPs) or manganese nanoparticles (MnNPs) on turkey spermatozoa preserved in a liquid state. Twenty turkey ejaculates were obtained from twenty healthy males. The collected semen was preserved at 4 °C for 48 h with or without NPs. Selected qualitative and quantitative parameters of sperm (motility, plasma membrane activity, mitochondrial membrane potential (MMP) and the percentage of sperm demonstrating NO and SOD activity) were examined after 2, 24 and 48 h of storage. Sperm motility and MMP decreased in semen preserved with ZnNPs at each time point of the analysis. However, all spermatozoa remained viable throughout storage. In contrast, membrane integrity and mitochondria activity (p ≤ 0.05) increased, and the highest SOD activity (p ≤ 0.05) was observed in semen preserved with MnNPs. The addition of MnNPs to the semen extender could potentially improve the parameters of turkey semen during prolonged storage.

Synthesis of Graphene oxide supported with Bimetallic nanoparticles and its Application

In this study, a very simple and highly effective mechanochemical preparation method was developed for the preparation of Ni nanoparticles supported graphene oxide (GO). The developed method is not only very simple and efficient, but also, the morphology of Ni/GO nanocomposites can be tuned by simply varying the metal loading. The nanoparticle has an immense assortment of prospective applications in biomedical, optical, and electronic fields. Nanoparticles are of great technological fascination as they are effectively an aqueduct between bulk substances and atomic or molecular structures. The properties of matter change as their size approaches to nanoscale and as the atomic percentage at the material surface becomes significant. For bulk materials larger than one micrometre in size, the rate of particles at the surface is minute relative to the total number of particles of the material. The absorbing and occasionally unpredicted properties of nanoparticles are not partly due to the characteristic of the material surface dominating the properties instead of the bulk properties. In this study, two different graphene oxide supported by two mono Ni Mn and bimetallic Ni\Mn nanoparticles catalyst synthesized. The size and shape of the products were characterised by scanning electron microscopy (SEM) andX-ray diffraction spectroscopy (XRD). Results proved that the newly developed graphene oxide carried nickel-manganese nanoparticles catalysts can be more efficient to reductive, oxidative and environmentally important organic pollutants.

Biosynthesis of Manganese Nanoparticles (MnNPs) from Brassica oleraceae (Cabbage leaves) and its Antibacterial Activity

Manganese nanoparticles are promising material for various applications such as water remediation, catalytic oxidation reactions, bio sensors, etc for their superior adsorbing, electrochemical, catalytic, magnetic, supercapactive like properties additionally, MnNPs possess significant medicinal values, hence, this research aims at synthesizing MnNPs biogenically, characterize them to explore antibacterial activity. Aqueous cabbage extract was allowed to react with precursor KMnO4 solution to synthesize the MnNPs. Then, MnNPs were characterized by: visual observation, UV-visible spectroscopy (UV-vis), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDX). Finally, agarwell diffusion was employed to study antibacterial activity against human pathogenic bacteria: staphylococcus aureus ATCC 25923, escherichia coli ATCC 25922 and salmonella typhi. Turning of purple color solution to reddish brown after stirring was the indication of the formation of nanoparticles. The formation of MnNPs was confirmed by appearance of sharp peak at 420 nm, the assigned band for MnNPs and the energy absorption band at 6 keV in EDX spectrum. Diffraction pattern of MnNPs revealed polycrystalline type with crystallite size of 10.70 nm. FT-IR result showed the chief biomolecules: flavoniods, alkaloids, proteins present in the extract which acts as reducing agent. FE-SEM confirmed the formation of spherical and ellipsoidal shaped agglomerated MnNPs. Agarwell diffusion method showed zone of inhibition (ZOI) of range 10-13 mm with (gram + ve and gram –ve) bacteria. Cabbage mediated biosynthesis of MnNPs is found simple, ecofriendly. This work revealed the formation of agglomerated MnNPs confirmed from uv-vis spectra and FE-SEM. Chief biomolecules of the extract act as reducing agent and stabilizing agent. Results of agarwell diffusion method depicted MnNPs are promising material for its antibacterial properties.

Effectiveness of manganese nanoparticles in the treatment of spring triticale seeds

In this work we studied the effect of finished preparative forms with manganese nanoparticles (monohydrate sulfate) with different contents and inclusion of stabilizers and solution modifiers on the growth and development of spring triticale plants in the first phases of ontogenesis. A positive effect of manganese on the increase of seed germination energy and germination of spring triticale at the dosage of Mn 20 mg/g + (PVP), with the drug consumption rate of 5 ml/t, was found. In this variant, both manganese and polyvinylpyrrolidone (PVP) solution stabilizer are determined to be positive. The inclusion of collagen hydrolysate (GC) and Ag-K modifier in the combination of the stabilizer solution, where the Mn content is 10 mg/g, negatively affected and reduced the germination energy by 13-17% compared to the control variant. Increasing the manganese content in the samples up to 20 mg/g levels out the negative effect of Ag-K modifier on seed germination. A positive effect within 7 days in most variants, both on the length of the sprout and on its weight was traced in the application of manganese with its content in the samples of 20 mg/g with a rate of 1 ml/t.