scholarly journals Detection of pathogens in blood or feces of adult horses with enteric disease and association with outcome of colitis

Author(s):  
Jamie J. Kopper ◽  
Jaclyn A. Willette ◽  
Clark J. Kogan ◽  
Alexis Seguin ◽  
Steven R. Bolin ◽  
...  
Keyword(s):  
Author(s):  
Bruce Wetzel ◽  
Robert Buscho ◽  
Raphael Dolin

It has been reported that explants of human fetal intestine can be maintained in culture for up to 21 days in a viable condition and that these organ cultures support the growth of a variety of known viral agents responsible for enteric disease. Scanning electron microscopy (SEM) has been undertaken on several series of these explants to determine their appearance under routine culture conditions.Fresh specimens of jejunum obtained from normal human fetuses were washed, dissected into l-4mm pieces, and cultured in modified Leibowitz L-15 medium at 34° C as previously described. Serial specimens were fixed each day in 3% glutaraldehyde for 90 minutes at room temperature, rinsed, dehydrated, and dried by the CO2 critical point method in a Denton DCP-1 device. Specimens were attached to aluminum stubs with 3M transfer tape No. 465, and one sample on each stub was carefully rolled along the adhesive such that villi were broken off to expose their interiors.


Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 656
Author(s):  
Konstantin Tanida ◽  
Andreas Hahn ◽  
Kirsten Alexandra Eberhardt ◽  
Egbert Tannich ◽  
Olfert Landt ◽  
...  

Microsporidiosis is an infection predominantly occurring in immunosuppressed patients and infrequently also in travelers. This study was performed to comparatively evaluate the diagnostic accuracy of real-time PCR assays targeting microsporidia with etiological relevance in the stool of human patients in a latent class analysis-based test comparison without a reference standard with perfect accuracy. Thereby, two one-tube real-time PCR assays and two two-tube real-time PCR assays targeting Enterocytozoon bieneusi and Encephalocytozoon spp. were included in the assessment with reference stool material (20), stool samples from Ghanaian HIV-positive patients (903), and from travelers, migrants and Colombian indigenous people (416). Sensitivity of the assays ranged from 60.4% to 97.4% and specificity from 99.1% to 100% with substantial agreement according to Cohen’s kappa of 79.6%. Microsporidia DNA was detected in the reference material and the stool of the HIV patients but not in the stool of the travelers, migrants, and the Colombian indigenous people. Accuracy-adjusted prevalence was 5.8% (n = 78) for the study population as a whole. In conclusion, reliable detection of enteric disease-associated microsporidia in stool samples by real-time PCR could be demonstrated, but sensitivity between the compared microsporidia-specific real-time PCR assays varied.


2008 ◽  
Vol 14 (2) ◽  
pp. 311-313 ◽  
Author(s):  
Craig W. Hedberg ◽  
Jesse F. Greenblatt ◽  
Bela T. Matyas ◽  
Jennifer Lemmings ◽  
Donald J. Sharp ◽  
...  

1985 ◽  
Vol 4 (1) ◽  
pp. 155-156
Author(s):  
Carmen Gutiérrez ◽  
Ernesto Guiraldes
Keyword(s):  

2013 ◽  
Vol 25 (3) ◽  
pp. 314-327 ◽  
Author(s):  
Kerry K. Cooper ◽  
J. Glenn Songer ◽  
Francisco A. Uzal
Keyword(s):  

Author(s):  
Elliot Spicer ◽  
BCIT School of Health Sciences, Environmental Health ◽  
Helen Heacock

Background: Seniors participate in sports to improve physical, mental, and social health; however, such activities may increase the risk of illness and injury. Curling is popular in this age group because it is physically manageable, strategic, and provides social connection. Certain factors in curling such as handshaking, play during the flu season, and shared contact with curling stones suggest an increased risk of disease transmission. The purpose of this study was to determine the qualitative risk of communicable enteric disease transmission due to shared contact with curling stone handles in a senior men’s curling league. Methods: 3M™ Quick Swabs were used to sample 22 curling stone handles for total coliforms before a senior’s league game. To analyze microbial shedding during gameplay, the same 22 handles were sampled after the game. Samples were plated on 3M™ Petrifilm™ Coliform Count Plates and incubated at 30ºC ± 1ºC for 24 hours ± 2 hours. Colonies were enumerated in units of CFU (colony forming units)/cm2. Ambient and handle surface temperatures were measured, and curler hygiene-related behaviours documented. Results: Total coliform counts for all samples were 0 CFU/cm2. The ambient temperature was 6.6°C pre-game, and 8.0°C post-game. Mean handle surface temperature was 3.6°C. Hygiene behaviours of concern were hand-face contact, handkerchief/tissue use, and handshaking. Conclusion: There is low risk of enteric disease transmission due to shared contact with curling stone handles by male curlers 55 years and older. Absence of coliforms may have been due to adequate player hygiene, transference of microbial load before sampling, error, or environmental conditions. Health promotion and education can reduce the infection risk elevated by poor hand hygiene, face contact, and handshaking in senior’s curling, thereby protecting the health and welfare of all participants.


2011 ◽  
Vol 174 (suppl 11) ◽  
pp. S23-S35 ◽  
Author(s):  
A. P. Wright ◽  
L. H. Gould ◽  
B. Mahon ◽  
M. J. Sotir ◽  
R. V. Tauxe

The Lancet ◽  
1907 ◽  
Vol 170 (4385) ◽  
pp. 794
Author(s):  
FrancisT. Bond
Keyword(s):  

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