scholarly journals A new mechanism for reduced sensitivity to demethylation-inhibitor fungicides in the fungal banana black Sigatoka pathogen Pseudocercospora fijiensis

2018 ◽  
Vol 19 (6) ◽  
pp. 1491-1503 ◽  
Author(s):  
Caucasella Diaz-Trujillo ◽  
Pablo Chong ◽  
Ioannis Stergiopoulos ◽  
Viviane Cordovez ◽  
Mauricio Guzman ◽  
...  
Keyword(s):  
Black Sigatoka ◽  
Pseudocercospora Fijiensis ◽  
Demethylation Inhibitor

scholarly journals First Draft Genome Sequence Resource of a Strain of Pseudocercospora fijiensis Isolated in North America

2020 ◽  
Vol 110 (10) ◽  
pp. 1620-1622
Author(s):  
Luis Amarillas ◽  
Mitzi Estrada-Acosta ◽  
Rubén G. León-Chan ◽  
Carlos López-Orona ◽  
Luis Lightbourn
Keyword(s):  
North America ◽  
Fungicide Resistance ◽  
Fungal Pathogens ◽  
Genomic Sequence ◽  
Draft Genome ◽  
Bioinformatic Analysis ◽  
Black Sigatoka ◽  
Efficient Management ◽  
Black Sigatoka Disease ◽  
Pseudocercospora Fijiensis

Black Sigatoka disease, caused by the fungus Pseudocercospora fijiensis, is one of the most devastating diseases of banana around the world. Fungicide applications are the primary tool used to manage black Sigatoka, but fungicide resistance in P. fijiensis, as in other fungal pathogens, is one of the major limitations in the efficient management and prevention of this disease. In the current study, we present the draft genome of P. fijiensis strain IIL-20, the first genomic sequence published from a strain of this fungus isolated in North America. Bioinformatic analysis showed putative genes involved in fungus virulence and fungicide resistance. These findings may lead us to a better understanding of the molecular pathogenesis of this fungal pathogen and also to the discovery of the mechanisms conferring fungicide resistance.

scholarly journals Combating a Global Threat to a Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) Genomes Reveal Clues for Disease Control

PLoS Genetics ◽  
2016 ◽  
Vol 12 (8) ◽  
pp. e1005876 ◽  
Author(s):  
Rafael E. Arango Isaza ◽  
Caucasella Diaz-Trujillo ◽  
Braham Dhillon ◽  
Andrea Aerts ◽  
Jean Carlier ◽  
...  
Keyword(s):  
Disease Control ◽  
Mycosphaerella Fijiensis ◽  
Black Sigatoka ◽  
Global Threat ◽  
Pseudocercospora Fijiensis

scholarly journals Population Structure of Pseudocercospora fijiensis in Costa Rica Reveals Shared Haplotype Diversity with Southeast Asian Populations

2017 ◽  
Vol 107 (12) ◽  
pp. 1541-1548 ◽  
Author(s):  
Amanda Saville ◽  
Melodi Charles ◽  
Suchitra Chavan ◽  
Miguel Muñoz ◽  
Luis Gómez-Alpizar ◽  
...  
Keyword(s):  
Costa Rica ◽  
Southeast Asia ◽  
Management Strategies ◽  
Haplotype Diversity ◽  
Costa Rican ◽  
Black Sigatoka ◽  
Shared Haplotype ◽  
Asian Populations ◽  
Haplotypic Diversity ◽  
Pseudocercospora Fijiensis

Pseudocercospora fijiensis is the causal pathogen of black Sigatoka, a devastating disease of banana that can cause 20 to 80% yield loss in the absence of fungicides in banana crops. The genetic structure of populations of P. fijiensis in Costa Rica was examined and compared with Honduran and global populations to better understand migration patterns and inform management strategies. In total, 118 isolates of P. fijiensis collected from Costa Rica and Honduras from 2010 to 2014 were analyzed using multilocus genotyping of six loci and compared with a previously published global dataset of populations of P. fijiensis. The Costa Rican and Honduran populations shared haplotype diversity with haplotypes from Southeast Asia, Oceania, and the Americas but not Africa for all but one of the six loci studied. Gene flow and shared haplotype diversity was found in Honduran and Costa Rican populations of the pathogen. The data indicate that the haplotypic diversity observed in Costa Rican populations of P. fijiensis is derived from dispersal from initial outbreak sources in Honduras and admixtures between genetically differentiated sources from Southeast Asia, Oceania, and the Americas.

scholarly journals Identification of New Hosts of Pseudocercospora fijiensis Suggests Innovative Pest Management Programs for Black Sigatoka Disease in Banana Plantations

Agronomy ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 666 ◽  
Author(s):  
Vázquez-Euán ◽  
Chi-Manzanero ◽  
Hernández-Velázquez ◽  
Tzec-Simá ◽  
Islas-Flores ◽  
...  
Keyword(s):  
Plant Species ◽  
Current Knowledge ◽  
Present Report ◽  
Black Sigatoka ◽  
Successful Management ◽  
Plant Host ◽  
Diagnostic Pcr ◽  
Banana Production ◽  
Initial Inoculum ◽  
Pseudocercospora Fijiensis

Black Sigatoka is the main constraint to banana production worldwide, and epidemic outbreaks are continuously causing huge losses. Successful management of diseases requires a profound knowledge of the epidemiological factors that influence disease dynamics. Information regarding alternative hosts of Pseudocercospora fijiensis, the causal agent, is still very scarce. To date, only Heliconia psittacorum has been reported as an alternative plant host, and we hypothesized that other plants can house P. fijiensis. In the present report, ten plant species with suspicious leaf spots were collected inside and around commercial banana crops in Mexico. Diagnostic PCR gave positive amplification for six of these plant species, and DNA sequencing confirmed the presence of the pathogen in four. This is the first report of the presence of P. fijiensis in unrelated plants and it represents a breakthrough in the current knowledge of black Sigatoka. This finding is very important given the polycyclic nature of this disease whose successful management requires the control of initial inoculum to minimize epidemic outbreaks. The results presented herein can be used to introduce innovations in integrated black Sigatoka management programs to reduce initial inoculum, and help the international initiative to reduce the use of fungicides in banana production.

scholarly journals Sources of resistance to Pseudocercospora fijiensis , the cause of black Sigatoka in banana

2021 ◽  
Author(s):  
Janet Kimunye ◽  
Evans Were ◽  
Rony Swennen ◽  
Altus Viljoen ◽  
George Mahuku
Keyword(s):  
Black Sigatoka ◽  
Sources Of Resistance ◽  
Pseudocercospora Fijiensis

scholarly journals Inelastic scattering imaging system for Pseudocercospora fijiensis detection

2019 ◽  
Author(s):  
Juan Velez Alvarez ◽  
Alvaro Bastidas ◽  
Alejandra Monsalve ◽  
Tehseen Adel ◽  
Isabel Calle ◽  
...  
Keyword(s):  
Inelastic Scattering ◽  
Imaging System ◽  
Culture Media ◽  
Black Sigatoka ◽  
Bending Vibration ◽  
Fungal Cell ◽  
Industrial Economy ◽  
Fungal Phytopathogen ◽  
Black Sigatoka Disease ◽  
Pseudocercospora Fijiensis

This work sought to develop an inelastic scattering imaging system based on Raman spectroscopy for the detection of the fungal phytopathogen, Pseudocercospora fijiensis, which causes Black Sigatoka disease in banana crops, very important in Colombian agro-industrial economy. This system consists of a modified stereoscope with an optical setup able to simultaneously capture spectral images together with its Raman spectra. The camera has two different bandpass filters attached, centered in the spectral region of C=O stretching of Chitin and the equatorial bending vibration of beta-1,3-glucan, molecules of the fungal cell wall. In this way, the system can get images with unique spectral features, suitable for training a convolutional neural network in order to get a recognition pattern of the fungal strain growing in the PDA agar. As a result, the instrument was able to detect the presence of P.fijiensis over the culture media.

Targeted and random genetic modification of the black Sigatoka pathogen Pseudocercospora fijiensis by Agrobacterium tumefaciens -mediated transformation

2018 ◽  
Vol 148 ◽  
pp. 127-137
Author(s):  
Caucasella Díaz-Trujillo ◽  
Adilson K. Kobayashi ◽  
Manoel Souza ◽  
Pablo Chong ◽  
Harold J.G. Meijer ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Modification ◽  
Black Sigatoka ◽  
Pseudocercospora Fijiensis

scholarly journals Genetic Diversity and Mating Type Distribution of Pseudocercospora fijiensis on Banana in Uganda and Tanzania

2020 ◽  
Author(s):  
Janet Njeri Kimunye ◽  
Norman Muzhinji ◽  
Diane Mostert ◽  
Altus Viljoen ◽  
Aletta E. van der Merwe ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
East Africa ◽  
Mating Type ◽  
Spatial Scales ◽  
Durable Resistance ◽  
Population Variation ◽  
Black Sigatoka ◽  
Single Spore ◽  
Type Distribution ◽  
Pseudocercospora Fijiensis

Black Sigatoka, caused by Pseudocercospora fijiensis, is a major foliar disease of banana and plantain worldwide. Little information on the genetic diversity and population structure of the pathogen is available for East Africa, which is needed to design effective and durable disease management strategies. We genotyped 319 single-spore isolates of P. fijiensis collected from seven regions in Uganda and Tanzania and five from Nigeria using 16 simple sequence repeat markers and mating type specific primers. Isolates from each country and region within country were treated as populations and subpopulations, respectively. A total of 296 multi-locus genotypes (MLGs) were recovered, representing a clonal fraction of 7%. Sub-populations had a moderate level of genetic diversity (Hexp = 0.12-0.31; mean 0.29). Mating type distribution did not deviate from equilibrium (MAT1-1: MAT1-2, 1:1 ratio) in Uganda, but in Tanzania the mating types were not in equilibrium (4:1 ratio). Index of association tests (IA and r̄d) showed that all populations were at linkage equilibrium (P>0.05), supporting the hypothesis of random association of alleles. These findings are consistent with a pathogen that reproduces both clonally and sexually. Low and insignificant levels of population differentiation were detected, with 90% of the variation occurring among isolates within subpopulations. The high intra-population variation has implications in breeding for resistance to P. fijiensis, as isolates differing in aggressiveness and virulence are likely to exist over small spatial scales. Diverse isolates will be required for resistance screening to ensure selection of banana cultivars with durable resistance to Sigatoka in East Africa

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