scholarly journals Study of natural diversity in response to a key pathogenicity regulator of Ralstonia solanacearum reveals new susceptibility genes in Arabidopsis thaliana

2021 ◽  
Author(s):  
Choghag Demirjian ◽  
Narjes Razavi ◽  
Henri Desaint ◽  
Fabien Lonjon ◽  
Stéphane Genin ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Ralstonia Solanacearum ◽  
Susceptibility Genes ◽  
Natural Diversity

scholarly journals Resistance and Susceptibility of Arabidopsis thaliana to Bacterial Wilt Caused by Ralstonia solanacearum

1998 ◽  
Vol 88 (4) ◽  
pp. 330-334 ◽  
Author(s):  
Chang-Hsien Yang ◽  
Gan-Der Ho
Keyword(s):  
Arabidopsis Thaliana ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Bacterial Colonization ◽  
Plant Diseases ◽  
Infection Site ◽  
Bacterial Isolation ◽  
Tomato Plants ◽  
Different Strains ◽  
Growth Of Bacteria

Tomato bacterial wilt caused by Ralstonia solanacearum is a model system for studying plant-bacterial interactions, because it is genetically one of the best characterized plant diseases. We demonstrate here that four different strains of R. solanacearum, two from radishes (Rd4 and Rd15) and two from tomato (Ps21 and Ps95), can infect 27 different ecotypes of Arabidopsis thaliana, causing different responses. All ecotypes tested were highly susceptible to strain Rd15, which caused symptoms similar to those observed in tomato plants. For example, leaf drooping and discoloration developed just 3 days after inoculation, and plants completely wilted within 1 week. Strains Rd4 and Ps95 were less infectious than Rd15. With these two strains, a variety of disease responses were observed among different ecotypes at 2 weeks after inoculation; both susceptible and resistant ecotypes of A. thaliana were identified. Ps21 was the least infectious of the four strains and caused almost no symptoms in any of the ecotypes of Arabidopsis tested. Direct bacterial isolation and plant skeleton hybridization analysis from infected plants indicated that bacterial colonization was correlated with the severity of symptoms. Growth of bacteria was limited to the infection site in resistant plants, whereas the bacteria spread throughout susceptible plants by 1 week after inoculation.

scholarly journals A natural diversity screen in Arabidopsis thaliana reveals determinants for HopZ1a recognition in the ZAR1‐ZED1 immune complex

2020 ◽  
Author(s):  
Maël Baudin ◽  
Eliza C. Martin ◽  
Chodon Sass ◽  
Jana A. Hassan ◽  
Claire Bendix ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Immune Complex ◽  
Natural Diversity

Prediction of protein–protein interactions between Ralstonia solanacearum and Arabidopsis thaliana

Amino Acids ◽  
2011 ◽  
Vol 42 (6) ◽  
pp. 2363-2371 ◽  
Author(s):  
Zhi-Gang Li ◽  
Fei He ◽  
Ziding Zhang ◽  
You-Liang Peng
Keyword(s):  
Arabidopsis Thaliana ◽  
Protein Interactions ◽  
Ralstonia Solanacearum ◽  
Protein Protein Interactions

scholarly journals Transcriptional Responses of Arabidopsis thaliana during Wilt Disease Caused by the Soil-Borne Phytopathogenic Bacterium, Ralstonia solanacearum

PLoS ONE ◽  
2008 ◽  
Vol 3 (7) ◽  
pp. e2589 ◽  
Author(s):  
Jian Hu ◽  
Xavier Barlet ◽  
Laurent Deslandes ◽  
Judith Hirsch ◽  
Dong Xin Feng ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Ralstonia Solanacearum ◽  
Wilt Disease ◽  
Transcriptional Responses ◽  
Phytopathogenic Bacterium

Deciphering the route of Ralstonia solanacearum colonization in Arabidopsis thaliana roots during a compatible interaction: focus at the plant cell wall

Planta ◽  
2012 ◽  
Vol 236 (5) ◽  
pp. 1419-1431 ◽  
Author(s):  
Catherine Digonnet ◽  
Yves Martinez ◽  
Nicolas Denancé ◽  
Marine Chasseray ◽  
Patrick Dabos ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Plant Cell ◽  
Ralstonia Solanacearum ◽  
Plant Cell Wall ◽  
Compatible Interaction

scholarly journals Natural Diversity in Flowering Responses of Arabidopsis thaliana Caused by Variation in a Tandem Gene Array

Genetics ◽  
2010 ◽  
Vol 186 (1) ◽  
pp. 263-276 ◽  
Author(s):  
Sarah Marie Rosloski ◽  
Sathya Sheela Jali ◽  
Sureshkumar Balasubramanian ◽  
Detlef Weigel ◽  
Vojislava Grbic
Keyword(s):  
Arabidopsis Thaliana ◽  
Gene Array ◽  
Natural Diversity

scholarly journals A Single Locus Leads to Resistance of Arabidopsis thaliana to Bacterial Wilt Caused by Ralstonia solanacearum Through a Hypersensitive-like Response

1999 ◽  
Vol 89 (8) ◽  
pp. 673-678 ◽  
Author(s):  
Gan-Der Ho ◽  
Chang-Hsien Yang
Keyword(s):  
Arabidopsis Thaliana ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Northern Blot Analysis ◽  
Plant Diseases ◽  
Glutathione S Transferase ◽  
Solanacearum Strain ◽  
Whole Plant ◽  
Pathogenesis Related Protein ◽  
Pathogenesis Related

Strains of Ralstonia solanacearum have been shown to cause bacterial wilt in some, but not all, ecotypes of Arabidopsis thaliana. We demonstrate here that after inoculation of the leaves of resistant ecotype S96 with R. solanacearum strain Ps95 necrosis around the inoculation site rapidly appeared and no further symptoms developed in the plant. Leaves of susceptible ecotype N913 completely wilted 7 days after inoculation with Ps95, and symptoms spread systemically throughout the whole plant within 2 weeks after inoculation. These results suggest that the resistance of Arabidopsis S96 to R. solanacearum is due to a response similar to the hypersensitive response (HR) observed in other plant diseases. Northern blot analysis of the expression of defense-related genes, known to be differentially induced during the HR in Arabidopsis, indicated that pathogenesis-related protein PR-1, glutathione S-transferase (GST1), and Cu, Zn superoxide dismutase (SOD) mRNAs increased significantly in S96 leaves between 3 to 12 h after infiltration with Ps95. The induction of these genes in susceptible ecotype N913 by Ps95 was clearly delayed. Genetic analysis of crosses between resistant ecotype S96 and susceptible ecotype N913 indicated that resistance to Ps95 is due to a single dominant locus.

scholarly journals Genetic Characterization of RRS1, a Recessive Locus in Arabidopsis thaliana that Confers Resistance to the Bacterial Soilborne Pathogen Ralstonia solanacearum

1998 ◽  
Vol 11 (7) ◽  
pp. 659-667 ◽  
Author(s):  
Laurent Deslandes ◽  
Frédéric Pileur ◽  
Laurence Liaubet ◽  
Sylvie Camut ◽  
Canan Can ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Ralstonia Solanacearum ◽  
Recombinant Inbred Lines ◽  
Recessive Trait ◽  
Dependent Manner ◽  
Resistant Accession ◽  
Genetic Cross ◽  
Recessive Locus ◽  
Bacterial Concentrations

The soilborne, vascular pathogen Ralstonia solanacearum, the causative agent of bacterial wilt, was shown to infect a range of Arabidopsis thaliana accessions. The pathogen was capable of infecting the Col-5 accession in an hrp-dependent manner, following root inoculation. Elevated bacterial population levels were found in leaves of Col-5, 4 to 5 days after root inoculation by the GMI1000 strain. Bacteria were found predominantly in the xylem vessels and spread systemically throughout the plant. The Nd-1 accession of A. thaliana was resistant to the GMI1000 strain of R. solanacearum. Bacterial concentrations detected in leaves of Nd-1, inoculated with an hrp+ strain of R. solanacearum, were only slightly higher than those detected in the susceptible accession, Col-5, following inoculation with a strain whose hrp gene cluster was deleted. Leaf inoculation of the GMI1000 strain on the resistant accession Nd-1 induced the formation of lesions in the older leaves of the rosette whereas the same strain of R. solanacearum provoked complete wilting of Col-5. Resistance to strain GMI1000 of R. solanacearum segregated as a simply inherited recessive trait in a genetic cross between Col-5 and Nd-1. F9 recombinant inbred lines generated between these two accessions were used to map a locus, RRS1, that was the major determinant of resistance between restriction fragment length polymorphism markers mi83 and mi61 on chromosome V. This region of the A. thaliana genome is known to contain many other pathogen recognition capabilities.

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