A mis‐regulated cyclic nucleotide‐gated channel mediates cytosolic calcium elevation and activates immunity in Arabidopsis

2021 ◽  
Author(s):  
Chunhui Zhao ◽  
Yinhua Tang ◽  
Junli Wang ◽  
Yanhong Zeng ◽  
Hequan Sun ◽  
...  
Keyword(s):  
Cyclic Nucleotide ◽  
Cytosolic Calcium ◽  
Gated Channel ◽  
Calcium Elevation

NO upregulation of a cyclic nucleotide-gated channel contributes to calcium elevation in endothelial cells

2002 ◽  
Vol 283 (4) ◽  
pp. C1080-C1089 ◽  
Author(s):  
Jianliang Zhang ◽  
Shen-Ling Xia ◽  
Edward R. Block ◽  
Jawaharlal M. Patel
Keyword(s):  
Endothelial Cells ◽  
Cyclic Nucleotide ◽  
Cytosolic Calcium ◽  
Calcium Entry ◽  
Mrna Levels ◽  
No Donor ◽  
Cng Channel ◽  
Gated Channel ◽  
Calcium Elevation ◽  
Porcine Pulmonary Artery

We investigated whether nitric oxide (NO) upregulates a cyclic nucleotide-gated (CNG) channel and whether this contributes to sustained elevation of intracellular calcium levels ([Ca2+]i) in porcine pulmonary artery endothelial cells (PAEC). Exposure of PAEC to an NO donor, NOC-18 (1 mM), for 18 h increased the protein and mRNA levels of CNGA2 40 and 50%, respectively ( P < 0.05). [Ca2+]iin NO-treated cells was increased 50%, and this increase was maintained for up to 12 h after removal of NOC-18 from medium. Extracellular calcium is required for the increase in [Ca2+]iin NO-treated cells. Thapsigargin induced a rapid cytosolic calcium rise, whereas both a CNG and a nonselective cation channel blocker caused a faster decline in [Ca2+]i, suggesting that capacitive calcium entry contributes to the elevated calcium levels. Antisense inhibition of CNGA2 expression attenuated the NO-induced increases in CNGA2 expression and [Ca2+]iand in capacitive calcium entry. Our results demonstrate that exogenous NO upregulates CNGA2 expression and that this is associated with elevated [Ca2+]iand capacitive calcium entry in porcine PAEC.

scholarly journals A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice

Cell Research ◽  
2019 ◽  
Vol 29 (10) ◽  
pp. 820-831 ◽  
Author(s):  
Jiachang Wang ◽  
Xi Liu ◽  
An Zhang ◽  
Yulong Ren ◽  
Fuqing Wu ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Cyclic Nucleotide ◽  
Cytoplasmic Calcium ◽  
Gated Channel ◽  
Calcium Elevation

scholarly journals Systemic administration of ivabradine, a hyperpolarization‐activated cyclic nucleotide‐gated channel inhibitor, blocks spontaneous absence seizures

Epilepsia ◽  
2021 ◽  
Author(s):  
Yasmine Iacone ◽  
Tatiana P. Morais ◽  
François David ◽  
Francis Delicata ◽  
Joanna Sandle ◽  
...  
Keyword(s):  
Cyclic Nucleotide ◽  
Systemic Administration ◽  
Absence Seizures ◽  
Gated Channel

scholarly journals Protection against hydrogen peroxide cytotoxicity in Rat-1 fibroblasts provided by the oncoprotein Bcl-2: maintenance of calcium homoeostasis is secondary to the effect of Bcl-2 on cellular glutathione

1999 ◽  
Vol 340 (1) ◽  
pp. 291-297 ◽  
Author(s):  
Matthäus M. RIMPLER ◽  
Ursula RAUEN ◽  
Thorsten SCHMIDT ◽  
Tarik MÖRÖY ◽  
Herbert DE GROOT
Keyword(s):  
Cell Death ◽  
Calcium Concentration ◽  
Cell Injury ◽  
Cytosolic Calcium ◽  
Glutathione Metabolism ◽  
Transfected Cells ◽  
Cytosolic Calcium Concentration ◽  
Glutathione Status ◽  
Calcium Elevation ◽  
Reduced State

The oncoprotein Bcl-2 protects cells against apoptosis, but the exact molecular mechanism that underlies this function has not yet been identified. Studying H2O2-induced cell injury in Rat-1 fibroblast cells, we observed that Bcl-2 had a protective effect against the increase in cytosolic calcium concentration and subsequent cell death. Furthermore, overexpression of Bcl-2 resulted in an alteration of cellular glutathione status: the total amount of cellular glutathione was increased by about 60% and the redox potential of the cellular glutathione pool was maintained in a more reduced state during H2O2 exposure compared with non-Bcl-2-expressing controls. In our cytotoxicity model, disruption of cellular glutathione homoeostasis closely correlated with the pathological elevation of cytosolic calcium concentration. Stabilization of the glutathione pool by Bcl-2, N-acetylcysteine or glucose delayed the cytosolic calcium increase and subsequent cell death, whereas depletion of glutathione by DL-buthionine-(S,R)-sulphoximine, sensitized Bcl-2-transfected cells towards cytosolic calcium increase and cell death. We therefore suggest that the protection exerted by Bcl-2 against H2O2-induced cytosolic calcium elevation and subsequent cell death is secondary to its effect on the cellular glutathione metabolism.

Expression of the olfactory cyclic nucleotide gated channel (CNG1) in the rat brain

Neuroreport ◽  
1995 ◽  
Vol 6 (10) ◽  
pp. 1459-1463 ◽  
Author(s):  
Alaa El-Din El-Husseini ◽  
Christopher Bladen ◽  
Steven R. Vincent
Keyword(s):  
Rat Brain ◽  
Cyclic Nucleotide ◽  
Gated Channel

Effect of loratadine, an anti-histamine drug, on cytosolic calcium elevation in rat peritoneal macrophages

1992 ◽  
Vol 26 ◽  
pp. 282
Author(s):  
O. Letari ◽  
A. Miozzo ◽  
G.E. Rovati ◽  
S. Nicosia
Keyword(s):  
Cytosolic Calcium ◽  
Peritoneal Macrophages ◽  
Calcium Elevation

Impaired Opsin Targeting and Cone Photoreceptor Migration in the Retina of Mice Lacking the Cyclic Nucleotide-Gated Channel CNGA3

2005 ◽  
Vol 46 (4) ◽  
pp. 1516 ◽  
Author(s):  
Stylianos Michalakis ◽  
Heidi Geiger ◽  
Silke Haverkamp ◽  
Franz Hofmann ◽  
Andrea Gerstner ◽  
...  
Keyword(s):  
Cyclic Nucleotide ◽  
Cone Photoreceptor ◽  
Gated Channel
Sign in / Sign up

Export Citation Format

Share Document