scholarly journals Mutually opposing activity of PIN7 splicing isoforms is required for auxin‐mediated tropic responses in Arabidopsis thaliana

2021 ◽  
Author(s):  
Ivan Kashkan ◽  
Mónika Hrtyan ◽  
Katarzyna Retzer ◽  
Jana Humpolíčková ◽  
Aswathy Jayasree ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Splicing Isoforms

scholarly journals Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana

2020 ◽  
Author(s):  
Ivan Kashkan ◽  
Mónika Hrtyan ◽  
Roberta Filepová ◽  
Zuzana Vondráková ◽  
Jan Hejátko ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Alternative Splicing ◽  
Transcriptome Sequencing ◽  
Subcellular Trafficking ◽  
Amino Acid Motif ◽  
Knock Out ◽  
Eukaryotic Genes ◽  
Evolutionarily Conserved ◽  
Splicing Isoforms ◽  
Functional Relevance

SUMMARYAdvanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, limited effort has been dedicated to investigating the functional relevance of particular splicing events, even those in the key developmental and hormonal regulators. Here we reveal, in the plant model Arabidopsis thaliana, that the PIN7 gene, which encodes a polarly localized transporter for the phytohormone auxin, produces two evolutionarily conserved transcripts. These isoforms PIN7a and PIN7b, differing in a 4 amino acid motif, are present at nearly equal levels in most cells, except some early developing tissues where the expression of PIN7b is moderately prevalent. Both proteins also transport auxin with similar capacity and directionality. However, only PIN7a but not PIN7b cDNA rescues the phenotypes associated with the pin7 knock-out mutation, consistent with their differences in the subcellular trafficking and dynamics at the plasma membrane. Further phenotypic analyses suggested a joint, mutually opposing activity of both isoforms as being required for correct seedling apical hook formation and auxin-mediated tropic responses. These results establish alternative splicing of the PIN family as an evolutionary conserved, functionally relevant mechanism, taking part in the auxin-mediated plant development.

Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta

1994 ◽  
Vol 5 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Seok So Chang ◽  
Soon Ki Park ◽  
Byung Chul Kim ◽  
Bong Joong Kang ◽  
Dal Ung Kim ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Genetic Transformation ◽  
In Planta

Localization of tandemly repeated DNA sequences in Arabidopsis thaliana

1991 ◽  
Vol 1 (2) ◽  
pp. 159-166 ◽  
Author(s):  
J. Maluszynska ◽  
J.S. Heslop-Harrison
Keyword(s):  
Arabidopsis Thaliana ◽  
Dna Sequences ◽  
Repeated Dna ◽  
Repeated Dna Sequences ◽  
Tandemly Repeated Dna

The effect of daylength on the transition to flowering in phytochrome-deficient, late-flowering and double mutants of Arabidopsis thaliana

1995 ◽  
Vol 95 (2) ◽  
pp. 260-266 ◽  
Author(s):  
Maarten Koornneef ◽  
Corrie Hanhart ◽  
Patty van Loenen-Martinet ◽  
Hetty Blankestijn de Vries
Keyword(s):  
Arabidopsis Thaliana ◽  
Late Flowering ◽  
Transition To Flowering

Control of carbohydrate metabolism in a starchless mutant of Arabidopsis thaliana

1992 ◽  
Vol 85 (3) ◽  
pp. 446-452
Author(s):  
Richard C. Sicher ◽  
Diane F. Kremer
Keyword(s):  
Arabidopsis Thaliana ◽  
Carbohydrate Metabolism ◽  
Starchless Mutant

Identification of a single-copy gene encoding a Type I chlorophyll a/b-binding polypeptide of photosystem I in Arabidopsis thaliana

1992 ◽  
Vol 84 (4) ◽  
pp. 561-567 ◽  
Author(s):  
Poul E. Jensen ◽  
Michael Kristensen ◽  
Tine Hoff ◽  
Jan Lehmbeck ◽  
Bjarne M. Stummann ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Chlorophyll A ◽  
Photosystem I ◽  
Single Copy ◽  
Type I ◽  
Single Copy Gene ◽  
Gene Encoding ◽  
Copy Gene

Ammonium uptake and cellular alkalisation in roots of Arabidopsis thaliana: The involvement of cytoplasmic calcium

2000 ◽  
Vol 110 (4) ◽  
pp. 518 ◽  
Author(s):  
Christoph Plieth ◽  
Burkhard Sattelmacher ◽  
Marc R. Knight
Keyword(s):  
Arabidopsis Thaliana ◽  
Ammonium Uptake ◽  
Cytoplasmic Calcium

Flavonoid profiling by LC-MS IN 14 – 3-3ν knockout mutants of Arabidopsis thaliana during drought stress conditions

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
F Nabbie ◽  
O Shperdheja ◽  
J Millot ◽  
J Lindberg ◽  
B Peethambaran
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Conditions ◽  
Knockout Mutants

BAHD-like malonyltransferase genes from Digitalis lanata and Arabidopsis thaliana and their putative role in cardenolide biosynthesis

2019 ◽  
Author(s):  
M Tropper ◽  
S Höhn ◽  
J Munkert ◽  
W Kreis
Keyword(s):  
Arabidopsis Thaliana ◽  
Digitalis Lanata ◽  
Putative Role
Sign in / Sign up

Export Citation Format

Share Document