Nine hundred and seventy-seven (977) cucumber (Cucumis sativus L.) accessions from the U.S. National Plant Germplasm System (NPGS) collection were tested for resistance to powdery mildew, caused by the fungus Podosphaera xanthii (Castagne) Braun and Shishkoff, formerly known as Sphaerotheca fuliginea (Schlecht.) Poll. Plants from each accession were evaluated in the greenhouse following inoculation with field isolates of P. xanthii. Each plant was placed into one of three susceptibility classes based on the amount of fungal growth and sporulation on the hypocotyl, stem, petioles and leaves - susceptible (S), intermediate (I) or resistant (R). Of the 977 accessions (9.6%), 94 contained at least one I or R-type plant. Seventeen of the 20 most-resistant accessions came from Asian sources, including China (PIs 418962, 418964, 432860, and 432870), India (PIs 197085, 197088, and 605930), Japan (PIs 279465, 288238, 390258, and 390266), Pakistan (PI 330628), the Philippines (PIs 426169 and 426170), and Taiwan (PIs 321006, 321009, and 321011). A quantitative study was conducted to compare mildew reproduction on S, I, and R-type cucumbers in the greenhouse and under field conditions in Ames, Iowa. Leaf disks were removed weekly and microscopic counts made of spore populations. The leaf disk method was superior to visual rating for ranking and differentiating intermediate from resistant accessions. Both the intermediate (moderately resistant) and highly resistant accessions provided excellent protection against powdery mildew in the field.