Genetic characterization of Listeria monocytogenes from ruminant listeriosis from different geographical regions in the U.S.

The prevalence and genetic diversity of Listeria monocytogenes in raw broiler legs at the retail level in Estonia were studied. A total of 240 raw broiler legs (120 from Estonia and 120 of foreign origin, which had been imported to Estonia from Denmark, Finland, Hungary, Sweden, and the United States) from 12 retail stores in the two largest cities in Estonia (Tallinn and Tartu) were investigated from January to December 2002. Of these, 70% were positive for L. monocytogenes. The prevalence of L. monocytogenes in broiler legs of Estonian origin (88%) was significantly higher than in broiler legs of foreign origin (53%) (P < 0.001). Altogether, 169 (106 Estonian and 63 imported) L. monocytogenes isolates were characterized by pulsed-field gel electrophoresis (PFGE) typing after treatment with the restriction enzyme AscI. The isolates showed a wide genetic diversity, with 35 different PFGE types obtained. Of these, 11 PFGE types came only from isolates of broiler legs of Estonian origin, 4 of Danish origin, 2 of Finnish origin, and 4 of Hungarian origin. Fourteen PFGE types came from isolates of broiler legs that originated from various countries. The strains that shared the same PFGE types from isolates of Estonian origin were recovered from broiler legs that came from different stores over the course of several months. Seventy-one L. monocytogenes isolates, including all PFGE types, were serotyped, and three serotypes (1/2a, 1/2b, and 4b) were obtained. Serotype 1/2a accounted for 96% of the isolates.

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Comparative genetic characterization of Listeria monocytogenes isolates from human and animal listeriosis cases

Microbiology ◽

10.1099/00221287-147-5-1095 ◽

2001 ◽

Vol 147 (5) ◽

pp. 1095-1104 ◽

Cited By ~ 154

Author(s):

Gregory T. Jeffers ◽

James L. Bruce ◽

Patrick L. McDonough ◽

Janet Scarlett ◽

Kathryn J. Boor ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Genetic Characterization

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Characterization of Listeria monocytogenes Recovered from Imported Cheese Contributed to the National PulseNet Database by the U.S. Food and Drug Administration from 2001 to 2008

Journal of Food Protection ◽

10.4315/0362-028x-73.8.1511 ◽

2010 ◽

Vol 73 (8) ◽

pp. 1511-1514 ◽

Cited By ~ 2

Author(s):

BABGALEH B. TIMBO ◽

CHRISTINE KEYS ◽

KARL KLONTZ

Keyword(s):

Listeria Monocytogenes ◽

Drug Administration ◽

Genetic Relatedness ◽

Tracking System ◽

Food And Drug Administration ◽

The United States ◽

Surveillance Network ◽

Imported Foods ◽

The U.S

Imported foods must meet the same U.S. Food and Drug Administration (FDA) standards as domestic foods. The FDA determines whether an imported food is in compliance with the Federal Food, Drug, and Cosmetic Act. Pursuant to its regulatory activities, the FDA conducts compliance surveillance on imported foods offered for entry into the U.S. commerce. The National PulseNet Database is the molecular surveillance network for foodborne infections and is widely used to provide real-time subtyping support to epidemiologic investigations of foodborne diseases. FDA laboratories use pulsed-field gel electrophoresis to subtype foodborne pathogens recovered from imported foods and submit the molecular patterns to the National PulseNet Database at the Centers for Disease Control and Prevention. There were 60 isolates of Listeria monocytogenes in the FDA Field Accomplishment and Compliance Tracking System from 2001 to 2008 due to cheese imported from the following countries: Mexico (n = 21 isolates), Italy (19), Israel (9), Portugal (5), Colombia (3), Greece (2), and Spain (1). We observed genetic diversity of L. monocytogenes isolates and genetic relatedness among strains recovered from imported cheese products coming to the United States from different countries. Consistent characterization of L. monocytogenes isolates recovered from imported cheeses, accompanied by epidemiologic investigations to ascertain human illness associated with these strains, could be helpful in the control of listeriosis acquired from imported cheeses.

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Prevalence, Antibiogram and Genetic Characterization of Listeria monocytogenes from Food Products in Egypt

Foods ◽

10.3390/foods10061381 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1381

Author(s):

Eman E. Abdeen ◽

Walid S. Mousa ◽

Ola. H. Harb ◽

Gehad A. Fath-Elbab ◽

Mohammed Nooruzzaman ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Genetic Characterization ◽

Food Products ◽

Raw Milk ◽

Food Samples ◽

World Health ◽

Fish Fillet ◽

Minced Meat ◽

The Impact

World Health Organization classified Listeria monocytogenes as a major notable foodborne pathogen associated with high mortality and hospitalization. The study reports the prevalence, antibiogram, virulence determination and genetic characterization of L. monocytogenes from different food products. A total of 250 food samples, fifty samples each from raw milk, ice cream, minced meat, fish fillet and sausage were collected from the Menoufiya governorate in Egypt. L. monocytogenes was detected in 17 (6.8%) of the tested food samples including minced meat (14%), fish fillet (8%), sausage (6%) and raw milk (6%). The antimicrobial susceptibility assay of 17 L. monocytogenes isolates against seventeen antibiotics belonging to eight antibiotics classes revealed a high susceptibility to norfloxacin (82.3%), amoxicillin-clavulanic acid (76.4%), cefotaxime (70.5%), erythromycin (64.6%), amoxicillin (64.6%), gentamicin (58.7%) and vancomycin (58.7%). While, high resistance was observed against oxytetracycline (76.4%), trimethoprim-sulfamethoxazole (76.4%), chloramphenicol (70.5%), doxycycline (64.6%), levofloxacin (41.2%) and azithromycin (41.2%). Of note, all L. monocytogenes isolates were multidrug-resistant. The multiplex PCR successfully amplified L. monocytogenes in all tested isolates. Screening of the five virulence-related genes revealed the hlyA and iap as the most prevalent genes followed by actA gene, however, the inlA and prfA genes were not detected in any of the studied isolates. The partial 16S rRNA gene sequencing of three L. monocytogenes isolates showed a high nucleotide similarity (99.1–99.8%) between the study isolates and various global clones, and phylogenetic analysis clustered these L. monocytogenes strains with other Listeria species including L. welshimeri, L. seeligeri and L. innocua. This study demonstrates the impact of L. monocytogenes as a major contaminant of various food products and suggests more attention to the awareness and hygienic measures in the food industry.

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Genetic characterization of clones of the bacterium Listeria monocytogenes causing epidemic disease.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.86.10.3818 ◽

1989 ◽

Vol 86 (10) ◽

pp. 3818-3822 ◽

Cited By ~ 194

Author(s):

J. C. Piffaretti ◽

H. Kressebuch ◽

M. Aeschbacher ◽

J. Bille ◽

E. Bannerman ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Genetic Characterization ◽

Epidemic Disease

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Genetic Characterization of a Listeria monocytogenes Serotype IVb Variant 1 Strain Isolated from Vegetal Matrix in Italy

Microbiology Resource Announcements ◽

10.1128/mra.00782-20 ◽

2020 ◽

Vol 9 (33) ◽

Author(s):

Marina Torresi ◽

Massimiliano Orsini ◽

Vicdalia Acciari ◽

Gabriella Centorotola ◽

Valeria Di Lollo ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Genetic Characterization ◽

Content Type

ABSTRACT We report the chromosome and plasmid sequences of a strain of Listeria monocytogenes IVb variant 1, a recently emerging serotype, isolated in Italy from ready-to-eat vegetables.

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Genetic Characterization of Plasmid-Associated Benzalkonium Chloride Resistance Determinants in a Listeria monocytogenes Strain from the 1998-1999 Outbreak

Applied and Environmental Microbiology ◽

10.1128/aem.02056-10 ◽

2010 ◽

Vol 76 (24) ◽

pp. 8231-8238 ◽

Cited By ~ 106

Author(s):

Driss Elhanafi ◽

Vikrant Dutta ◽

Sophia Kathariou

Keyword(s):

Listeria Monocytogenes ◽

Food Processing ◽

Benzalkonium Chloride ◽

Genetic Basis ◽

Genetic Characterization ◽

Chloride Resistance ◽

Processing Plants ◽

Resistant Strains ◽

Ammonium Compounds

ABSTRACT Quaternary ammonium compounds such as benzalkonium chloride (BC) are widely used as disinfectants in both food processing and medical environments. BC-resistant strains of Listeria monocytogenes have been implicated in multistate outbreaks of listeriosis and have been frequently isolated from food processing plants. However, the genetic basis for BC resistance in L. monocytogenes remains poorly understood. In this study, we have characterized a plasmid (pLM80)-associated BC resistance cassette in L. monocytogenes H7550, a strain implicated in the 1998-1999 multistate outbreak involving contaminated hot dogs. The BC resistance cassette (bcrABC) restored resistance to BC (MIC, 40 μg/ml) in a plasmid-cured derivative of H7550. All three genes of the cassette were essential for imparting BC resistance. The transcription of H7550 BC resistance genes was increased under sublethal (10 μg/ml) BC exposure and was higher at reduced temperatures (4, 8, or 25°C) than at 37°C. The level of transcription was higher at 10 μg/ml than at 20 or 40 μg/ml. In silico analysis suggested that the BC resistance cassette was harbored by an IS1216 composite transposon along with other genes whose functions are yet to be determined. The findings from this study will further our understanding of the adaptations of this organism to disinfectants such as BC and may contribute to the elucidation of possible BC resistance dissemination in L. monocytogenes.

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