scholarly journals An Ethenoadenine FAD Analog Accelerates UV Dimer Repair by DNA Photolyase

2018 ◽  
Vol 94 (1) ◽  
pp. 195-195
Author(s):  
Madhavan Narayanan ◽  
Vijay R. Singh ◽  
Goutham Kodali ◽  
Katarina Moravcevic ◽  
Robert J. Stanley
Keyword(s):  
Dna Photolyase

scholarly journals Action mechanism of Escherichia coli DNA photolyase. II. Role of the chromophores in catalysis.

1987 ◽  
Vol 262 (1) ◽  
pp. 486-491 ◽  
Author(s):  
M S Jorns ◽  
E T Baldwin ◽  
G B Sancar ◽  
A Sancar
Keyword(s):  
Escherichia Coli ◽  
Action Mechanism ◽  
Dna Photolyase

scholarly journals Gene diversity explains variation in biological features of insect killing fungus, Beauveria bassiana

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Laila Gasmi ◽  
Sehyeon Baek ◽  
Jong Cheol Kim ◽  
Sihyeon Kim ◽  
Mi Rong Lee ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Beauveria Bassiana ◽  
Gene Diversity ◽  
Dna Photolyase ◽  
Cyclophilin B ◽  
Biological Features ◽  
Number Variation ◽  
Potential Impact ◽  
Chitinase Genes ◽  
Fungus Beauveria Bassiana

AbstractBeauveria bassiana is a species complex whose isolates show considerable natural genetic variability. However, little is known about how this genetic diversity affects the fungus performance. Herein, we characterized the diversity of genes involved in various mechanisms of the infective cycle of 42 isolates that have different growth rates, thermotolerance and virulence. The analysed genes showed general genetic diversity measured as non-synonymous changes (NSC) and copy number variation (CNV), with most of them being subjected to positive episodic diversifying selection. Correlation analyses between NSC or CNV and the isolate virulence, thermotolerance and growth rate revealed that various genes shaped the biological features of the fungus. Lectin-like, mucin signalling, Biotrophy associated and chitinase genes NSCs correlated with the three biological features of B. bassiana. In addition, other genes (i.e. DNA photolyase and cyclophilin B) that had relatively conserved sequences, had variable CNs across the isolates which were correlated with the variability of either virulence or thermotolerance of B. bassiana isolates. The data obtained is important for a better understanding of population structure, ecological and potential impact when isolates are used as mycoinsecticides and can justify industrialization of new isolates.

Toward an Artificial Oxidative DNA Photolyase

2004 ◽  
Vol 69 (2) ◽  
pp. 543-548 ◽  
Author(s):  
Mickaël Pauvert ◽  
Patrick Laine ◽  
Marco Jonas ◽  
Olaf Wiest
Keyword(s):  
Dna Photolyase

A QM/MM Investigation of Thymine Dimer Radical Anion Splitting Catalyzed by DNA Photolyase

ChemPhysChem ◽  
2009 ◽  
Vol 10 (2) ◽  
pp. 400-410 ◽  
Author(s):  
Fanny Masson ◽  
Teodoro Laino ◽  
Ursula Rothlisberger ◽  
Jürg Hutter
Keyword(s):  
Radical Anion ◽  
Dna Photolyase ◽  
Thymine Dimer

scholarly journals Development of the Multiple Gene Knockout System with One-Step PCR in Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius

Archaea ◽  
2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Shoji Suzuki ◽  
Norio Kurosawa
Keyword(s):  
Gene Knockout ◽  
Arginine Decarboxylase ◽  
Sulfolobus Acidocaldarius ◽  
Dna Photolyase ◽  
Genetic Studies ◽  
Multiple Gene ◽  
Plasmid Construction ◽  
Pcr Product ◽  
Optimized Protocol ◽  
One Step

Multiple gene knockout systems developed in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius are powerful genetic tools. However, plasmid construction typically requires several steps. Alternatively, PCR tailing for high-throughput gene disruption was also developed in S. acidocaldarius, but repeated gene knockout based on PCR tailing has been limited due to lack of a genetic marker system. In this study, we demonstrated efficient homologous recombination frequency (2.8 × 104 ± 6.9 × 103 colonies/μg DNA) by optimizing the transformation conditions. This optimized protocol allowed to develop reliable gene knockout via double crossover using short homologous arms and to establish the multiple gene knockout system with one-step PCR (MONSTER). In the MONSTER, a multiple gene knockout cassette was simply and rapidly constructed by one-step PCR without plasmid construction, and the PCR product can be immediately used for target gene deletion. As an example of the applications of this strategy, we successfully made a DNA photolyase- (phr-) and arginine decarboxylase- (argD-) deficient strain of S. acidocaldarius. In addition, an agmatine selection system consisting of an agmatine-auxotrophic strain and argD marker was also established. The MONSTER provides an alternative strategy that enables the very simple construction of multiple gene knockout cassettes for genetic studies in S. acidocaldarius.

Femtosecond Dynamics of Flavin Cofactor in DNA Photolyase:  Radical Reduction, Local Solvation, and Charge Recombination

2005 ◽  
Vol 109 (4) ◽  
pp. 1329-1333 ◽  
Author(s):  
Haiyu Wang ◽  
Chaitanya Saxena ◽  
Donghui Quan ◽  
Aziz Sancar ◽  
Dongping Zhong
Keyword(s):  
Charge Recombination ◽  
Dna Photolyase ◽  
Femtosecond Dynamics ◽  
Radical Reduction

Evaluation of homology between cloned Escherichia coli and yeast DNA photolyase genes and higher eukaryotic genomes

1986 ◽  
Vol 166 (2) ◽  
pp. 143-147 ◽  
Author(s):  
Paul J. Meechan ◽  
Kathryn M. Milam ◽  
James E. Cleaver
Keyword(s):  
Escherichia Coli ◽  
Dna Photolyase ◽  
Eukaryotic Genomes

Identification of oligothymidylates as new simple substrates for E. coli DNA photolyase and their use in a rapid spectrophotometric enzyme assay

Biochemistry ◽  
1985 ◽  
Vol 24 (8) ◽  
pp. 1856-1861 ◽  
Author(s):  
Marilyn Schuman Jorns ◽  
Gwendolyn B. Sancar ◽  
Aziz Sancar
Keyword(s):  
Enzyme Assay ◽  
Dna Photolyase ◽  
E Coli

scholarly journals Identification of DNA Photolyase Gene of a Drought Tolerance Branching Line of Kenaf (<i>Hibiscus cannabinus</i> L.)

2012 ◽  
Vol 03 (06) ◽  
pp. 810-815
Author(s):  
Estri Laras Arumingtyas ◽  
Nur Basuki ◽  
Sutiman Bambang Sumitro ◽  
Sudjindro  
Keyword(s):  
Drought Tolerance ◽  
Hibiscus Cannabinus ◽  
Dna Photolyase
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