Spectrofluorometric Insights into the Application of PAM Fluorometry in Photosynthetic Research

2021 ◽  
Author(s):  
Suhailar Sma‐Air ◽  
Raymond J. Ritchie
Keyword(s):  
Pam Fluorometry

scholarly journals A Novel Thin-Film Technique to Improve Accuracy of Fluorescence-Based Estimates for Periphytic Biofilms

Water ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1464
Author(s):  
Leon Katona ◽  
Yvonne Vadeboncoeur ◽  
Christopher T. Nietch ◽  
Katie Hossler
Keyword(s):  
Thin Film ◽  
Thin Films ◽  
Light Attenuation ◽  
Pulse Amplitude ◽  
Light Signal ◽  
Photosynthetic Parameters ◽  
Pam Fluorometry ◽  
Attenuation Coefficients ◽  
Thin Film Technique ◽  
Relative Errors

Recent studies suggest that photophysiological parameters for intact substrates with depth (e.g., periphytic biofilms, microphytobenthos) are overestimated by pulse-amplitude modulated (PAM) fluorometry. This overestimation results from depth-integration effects, following the activation of deeper photosynthesizing layers by an attenuated light signal. To mitigate this error, we propose a novel slide-based thin-film technique in which fluorescence is measured on a vertically representative subsample of the biofilm, spread evenly on a microscope slide. We compared bias and precision for photosynthetic parameters estimated through conventional PAM fluorometry on intact biofilms and through our novel slide-based technique, both theoretically and empirically. Numerical simulations confirmed the consistent overestimation of key parameters for intact biofilms, with relative errors up to 145%, compared to, at most, 52% on thin films. Paired empirical observations likewise demonstrated that estimates based on intact biofilms were consistently higher (up to 248%, p<0.001) than estimates from thin films. Numerical simulation suggested greater precision with the slide-based technique for homogeneous biofilms, but potentially less precision for heterogeneous biofilms with improper subsampling. Our empirical comparison, however, demonstrated some improvement in precision with the slide-based technique (e.g., the coefficient of variation for the maximum electron transport rate was reduced 30%, p=0.009). We recommend the use of the slide-based technique, particularly for biofilms that are thick or have small light attenuation coefficients. Care should be taken, however, to obtain vertically representative subsamples of the biofilm for measurement.

Electron transport in Tradescantia leaves acclimated to high and low light: thermoluminescence, PAM-fluorometry, and EPR studies

2020 ◽  
Vol 146 (1-3) ◽  
pp. 123-141
Author(s):  
Olesya A. Kalmatskaya ◽  
Boris V. Trubitsin ◽  
Igor S. Suslichenko ◽  
Vladimir A. Karavaev ◽  
Alexander N. Tikhonov
Keyword(s):  
Electron Transport ◽  
Pam Fluorometry ◽  
Low Light

Use of pulse amplitude modulated (PAM) fluorometry for in situ measurements of photosynthesis in two Red Sea faviid corals

1998 ◽  
Vol 131 (4) ◽  
pp. 607-612 ◽  
Author(s):  
S. Beer ◽  
M. Ilan ◽  
A. Eshel ◽  
A. Weil ◽  
I. Brickner
Keyword(s):  
Red Sea ◽  
Pulse Amplitude ◽  
In Situ Measurements ◽  
Pam Fluorometry

Sensitivity evaluation of the green alga Chlamydomonas reinhardtii to uranium by pulse amplitude modulated (PAM) fluorometry

2013 ◽  
Vol 140-141 ◽  
pp. 288-294 ◽  
Author(s):  
Olivier Herlory ◽  
Jean-Marc Bonzom ◽  
Rodolphe Gilbin
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Green Alga ◽  
Pulse Amplitude ◽  
Pam Fluorometry ◽  
Sensitivity Evaluation

scholarly journals CMOS-based Image Cytometry for Detection of Phytoplankton in Ballast Water

2017 ◽  
Author(s):  
M Jofre ◽  
J M Perez ◽  
P Martinez ◽  
Z Moubarak ◽  
C Hurth ◽  
...  
Keyword(s):  
The Netherlands ◽  
Ballast Water ◽  
Wadden Sea ◽  
Limit Of Detection ◽  
Water Environment ◽  
Image Cytometry ◽  
Marine Water ◽  
Pam Fluorometry ◽  
Ballast Water Treatment Systems ◽  
Fresh Water Environment

An image cytometer (CYT) for the analysis of phytoplankton in fresh and marine water environments is introduced. A linear quantification of the number of cells over several orders of magnitude of concentrations was observed using cultures of Tetraselmis and Nannochloropsis measured by autofluorescence of the chlorophyll in a laboratory environment. The functionality of the system outside the laboratory was analysed by phytoplankton quantification of samples taken from marine water environment (Dutch Wadden Sea, The Netherlands) and fresh water environment (Lake Ijssel, The Netherlands). The CYT was also employed to study the effects of two ballast water treatment systems (BWTS), based on chlorine electrolysis and UV sterilisation by determining the vitality of the phytoplankton. In order to ensure the detection limit, a large volume (1l) of samples was collected and concentrated to 3 ml using CelltrapTM filters. The results were compared to benchmarked flow cytometer and PAM Fluorometry at Marine Eco-Analytics (MEA-NL). The image cytometer reached a 10 cells/ml limit of detection (LoD) with an accuracy between 0.7 and 0.5 log, and a correlation of 88.29% in quantification and 96.21% in vitality, when compared to benchmarked monitoring techniques.

Photosynthetic rates ofUlva(Chlorophyta) measured by pulse amplitude modulated (PAM) fluorometry

2000 ◽  
Vol 35 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Sven Beer ◽  
Christer Larsson ◽  
Orna Poryan ◽  
Lennart Axelsson
Keyword(s):  
Pulse Amplitude ◽  
Pam Fluorometry ◽  
Photosynthetic Rates

scholarly journals Polychromatic Fourier-PAM fluorometry and hyperspectral analysis of chlorophyll fluorescence from Phaseolus vulgaris leaves: Effects of green light

2020 ◽  
Vol 7 (2) ◽  
pp. 204-211 ◽  
Author(s):  
Vladimir Lysenko ◽  
Ya Guo ◽  
Alexey Kosolapov ◽  
Elena Usova ◽  
Tatyana Varduny ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Phaseolus Vulgaris ◽  
Green Light ◽  
Pam Fluorometry
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