Podophyllotoxin‐combined 5‐aminolevulinic acid photodynamic therapy significantly promotes HR‐HPV‐infected cell death

2021 ◽  
Author(s):  
Jingying Wang ◽  
Qi Wang ◽  
Pingjiao Chen ◽  
Qian Li ◽  
Zhijia Li ◽  
...  
Keyword(s):  
Cell Death ◽  
Photodynamic Therapy ◽  
Infected Cell ◽  
Aminolevulinic Acid

Aminolevulinic Acid-Mediated Photodynamic Therapy Causes Cell Death in MG-63 Human Osteosarcoma Cells

2016 ◽  
Vol 34 (9) ◽  
pp. 400-405 ◽  
Author(s):  
Bradley White ◽  
Vince Rossi ◽  
Paige J. Baugher
Keyword(s):  
Cell Death ◽  
Photodynamic Therapy ◽  
Aminolevulinic Acid ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

Ascorbic acid suppresses cell death in rat DS-sarcoma cancer cells induced by 5-aminolevulinic acid-based photodynamic therapy

2006 ◽  
Vol 40 (5) ◽  
pp. 827-836 ◽  
Author(s):  
Juergen Frank ◽  
Andrea Flaccus ◽  
Christine Schwarz ◽  
Christine Lambert ◽  
Hans K. Biesalski
Keyword(s):  
Cell Death ◽  
Ascorbic Acid ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Aminolevulinic Acid

scholarly journals Experimental investigation of a combinational iron chelating protoporphyrin IX prodrug for fluorescence detection and photodynamic therapy

2021 ◽  
Author(s):  
Anette Magnussen ◽  
Charlotte Reburn ◽  
Alexis Perry ◽  
Mark Wood ◽  
Alison Curnow
Keyword(s):  
Cell Death ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Aminolevulinic Acid ◽  
Protoporphyrin Ix ◽  
Iron Chelation ◽  
Squamous Carcinoma ◽  
Chelating Agent ◽  
Neutral Red ◽  
Squamous Carcinoma Cells

AbstractPhotodynamic therapy (PDT) is an oxygen-dependent, light-activated, and locally destructive drug treatment of cancer. Protoporphyrin IX (PpIX)-induced PDT exploits cancer cells’ own innate heme biosynthesis to hyper-accumulate the naturally fluorescent and photoactive precursor to heme, PpIX. This occurs as a result of administering heme precursors (e.g., aminolevulinic acid; ALA) because the final step of the pathway (the insertion of ferrous iron into PpIX by ferrochelatase to form heme) is relatively slow. Separate administration of an iron chelating agent has previously been demonstrated to significantly improve dermatological PpIX-PDT by further limiting heme production. A newly synthesized combinational iron chelating PpIX prodrug (AP2-18) has been assessed experimentally in cultured primary human cells of bladder and dermatological origin, as an alternative photosensitizing agent to ALA or its methyl or hexyl esters (MAL and HAL respectively) for photodetection/PDT. Findings indicated that the technique of iron chelation (either through the separate administration of the established hydroxypyridinone iron chelator CP94 or the just as effective combined AP2-18) did not enhance either PpIX fluorescence or PDT-induced (neutral red assessed) cell death in human primary normal and malignant bladder cells. However, 500 µM AP2-18 significantly increased PpIX accumulation and produced a trend of increased cell death within epithelial squamous carcinoma cells. PpIX accumulation destabilized the actin cytoskeleton in bladder cancer cells prior to PDT and resulted in caspase-3 cleavage/early apoptosis afterwards. AP2-18 iron chelation should continue to be investigated for the enhancement of dermatological PpIX-PDT applications but not bladder photodetection/PDT.

Massive apoptotic cell death of human glioma cells via a mitochondrial pathway following 5-aminolevulinic acid-mediated photodynamic therapy

2007 ◽  
Vol 83 (3) ◽  
pp. 223-231 ◽  
Author(s):  
Hiroto Inoue ◽  
Yoshinaga Kajimoto ◽  
Masa-Aki Shibata ◽  
Norio Miyoshi ◽  
Naoko Ogawa ◽  
...  
Keyword(s):  
Cell Death ◽  
Photodynamic Therapy ◽  
Aminolevulinic Acid ◽  
Apoptotic Cell ◽  
Glioma Cells ◽  
Mitochondrial Pathway ◽  
Apoptotic Cell Death ◽  
Human Glioma ◽  
Human Glioma Cells

scholarly journals Systemic MEK inhibition enhances the efficacy of 5-aminolevulinic acid-photodynamic therapy

2019 ◽  
Vol 121 (9) ◽  
pp. 758-767 ◽  
Author(s):  
Vipin Shankar Chelakkot ◽  
Jayoti Som ◽  
Ema Yoshioka ◽  
Chantel P. Rice ◽  
Suzette G. Rutihinda ◽  
...  
Keyword(s):  
Cell Death ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Aminolevulinic Acid ◽  
Combined Therapy ◽  
Combined Treatment ◽  
Mek Inhibitor ◽  
Ros Generation ◽  
Strong Basis ◽  
Mek Inhibition

Abstract Background Protoporphyrin IX (PpIX) gets accumulated preferentially in 5-aminolevulinic acid (5-ALA)-treated cancer cells. Photodynamic therapy (PDT) utilises the accumulated PpIX to trigger cell death by light-induced generation of reactive oxygen species (ROS). We previously demonstrated that oncogenic Ras/MEK decreases PpIX accumulation in cancer cells. Here, we investigated whether combined therapy with a MEK inhibitor would improve 5-ALA-PDT efficacy. Methods Cancer cells and mice models of cancer were treated with 5-ALA-PDT, MEK inhibitor or both MEK inhibitor and 5-ALA-PDT, and treatment efficacies were evaluated. Results Ras/MEK negatively regulates the cellular sensitivity to 5-ALA-PDT as cancer cells pre-treated with a MEK inhibitor were killed more efficiently by 5-ALA-PDT. MEK inhibition promoted 5-ALA-PDT-induced ROS generation and programmed cell death. Furthermore, the combination of 5-ALA-PDT and a systemic MEK inhibitor significantly suppressed tumour growth compared with either monotherapy in mouse models of cancer. Remarkably, 44% of mice bearing human colon tumours showed a complete response with the combined treatment. Conclusion We demonstrate a novel strategy to promote 5-ALA-PDT efficacy by targeting a cell signalling pathway regulating its sensitivity. This preclinical study provides a strong basis for utilising MEK inhibitors, which are approved for treating cancers, to enhance 5-ALA-PDT efficacy in the clinic.

In vitro study of cell death with 5-aminolevulinic acid based photodynamic therapy to improve the efficiency of cancer treatment

Laser Physics ◽  
2012 ◽  
Vol 22 (3) ◽  
pp. 626-633 ◽  
Author(s):  
S. Firdous ◽  
M. Nawaz ◽  
M. Ikram ◽  
M. Ahmed
Keyword(s):  
Cell Death ◽  
Photodynamic Therapy ◽  
Cancer Treatment ◽  
Aminolevulinic Acid ◽  
In Vitro Study ◽  
Vitro Study

Abstract 3464: Aminolevulinic acid-mediated photodynamic therapy induces cell death in human osteosarcoma cells

2012 ◽  
Author(s):  
Mariko Newton ◽  
Bradley White ◽  
Vincent Rossi ◽  
Paige J. Baugher
Keyword(s):  
Cell Death ◽  
Photodynamic Therapy ◽  
Aminolevulinic Acid ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells
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