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2022 ◽  
Vol 2152 (1) ◽  
pp. 012064
Author(s):  
Junfeng Xu ◽  
Taoying Wang ◽  
Zhipeng Xue

Abstract In the operation of direct air-cooled units, there are many factors that affect the safety and economy of the unit. The “hot air recirculation” and “backfill” phenomena caused by lateral wind, the influence of high ambient wind on the heat transfer performance of the air cooling unit near the windshield wall, and the “hot air recirculation” phenomenon caused by the back wind of the furnace, these may affect the direct air cooling unit. Safe and economical operation. Through carrying out on-site test research, collecting the actual operating conditions of the unit operation, the organic combination of laboratory simulation data and actual data is provided to provide a strong basis for the development of corresponding technologies.


2021 ◽  
Vol 10 (1) ◽  
pp. 70
Author(s):  
Eugene Rosenberg ◽  
Ilana Zilber-Rosenberg

Microbiomes are transmitted between generations by a variety of different vertical and/or horizontal modes, including vegetative reproduction (vertical), via female germ cells (vertical), coprophagy and regurgitation (vertical and horizontal), physical contact starting at birth (vertical and horizontal), breast-feeding (vertical), and via the environment (horizontal). Analyses of vertical transmission can result in false negatives (failure to detect rare microbes) and false positives (strain variants). In humans, offspring receive most of their initial gut microbiota vertically from mothers during birth, via breast-feeding and close contact. Horizontal transmission is common in marine organisms and involves selectivity in determining which environmental microbes can colonize the organism’s microbiome. The following arguments are put forth concerning accurate microbial transmission: First, the transmission may be of functions, not necessarily of species; second, horizontal transmission may be as accurate as vertical transmission; third, detection techniques may fail to detect rare microbes; lastly, microbiomes develop and reach maturity with their hosts. In spite of the great variation in means of transmission discussed in this paper, microbiomes and their functions are transferred from one generation of holobionts to the next with fidelity. This provides a strong basis for each holobiont to be considered a unique biological entity and a level of selection in evolution, largely maintaining the uniqueness of the entity and conserving the species from one generation to the next.


Author(s):  
Victoria B. Ornopia ◽  
Ma. Melanie N. Edig ◽  
Ronald S. Decano

Schools worldwide shuttered their doors in the spring of 2020 to slow the spread of the COVID -19 pandemic virus. This physical closure quickly moved to modular learning, which increased parental and guardian responsibilities. Data were collected from 10 single parents of Rizal Memorial Colleges, Inc. The research design of this study utilized the phenomenological method to determine the Melancholic sentiments towards modular learning modality: single parents in focus in The Rizal Memorial Colleges, Inc. This study aimed to investigate parents’ experiences and struggles by the use of thematic coding with the use of essential themes. Data obtained identified emergent themes clustered, namely; strive to balance parent employment demands and learner needs, strenuous assisting more than one child in the home with learning, lack of personal balance, and parent feels overwhelmed; maintaining a strict schedule; engaging in creative activities; keeping children busy and; organizing daily routine; tracking academic progress, keeping socio-emotional Development, accessing of achievement, and identifying emotional toll. The findings revealed and addressed the learning gap that has emerged in their children’s learning in these challenging times. Furthermore, single parents, being aware of how to manage their time, struggles, hardship, and challenges to cope with their sentiment experiences, and strive on how they can surpass and would be a strong basis for school administrators as this comes as an opportunity to evaluate the effectiveness of different educational approaches and develop teaching and learning process suited to specific educational needs. Considering the result of this study as input from families, and should be sought to create a program and learning method that is beneficial to all school stakeholders. KEYWORDS: Melancholic sentiments, Modular learning modality, and Single Parents


PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0260902
Author(s):  
Alessandro Cherubini ◽  
Francesco Rusconi ◽  
Lorenza Lazzari

In the last few years, there has been a considerable increase in the use of organoids, which is a new three-dimensional culture technology applied in scientific research. The main reasons for their extensive use are their plasticity and multiple applications, including in regenerative medicine and the screening of new drugs. The aim of this study was to better understand these structures by focusing on the choice of the best housekeeping gene (HKG) to perform accurate molecular analysis on such a heterogeneous system. This feature should not be underestimated because the inappropriate use of a HKG can lead to misleading data and incorrect results, especially when the subject of the study is innovative and not totally explored like organoids. We focused our attention on the newly described human pancreatic organoids (hPOs) and compared 12 well-known HKGs (ACTB, B2M, EF1α, GAPDH, GUSB, HPRT, PPIA, RNA18S, RPL13A TBP, UBC and YWHAZ). Four different statistical algorithms (NormFinder, geNorm, BestKeeper and ΔCt) were applied to estimate the expression stability of each HKG, and RefFinder was used to identify the most suitable genes for RT-qPCR data normalization. Our results showed that the intragroup and intergroup comparisons could influence the best choice of the HKG, making clear that the identification of a stable reference gene for accurate and reproducible RT-qPCR data normalization remains a critical issue. In summary, this is the first report on HKGs in human organoids, and this work provides a strong basis to pave the way for further gene analysis in hPOs.


2021 ◽  
Vol 9 ◽  
Author(s):  
Nicolas Montagné ◽  
Muriel Jager ◽  
Thomas Chertemps ◽  
Emma Persyn ◽  
Yan Jaszczyszyn ◽  
...  

Insects astoundingly dominate Earth’s land ecosystems and have a huge impact on human life. Almost every aspect of their life relies upon their highly efficient and adaptable chemosensory system. In the air, most chemical signals that are detected at long range are hydrophobic molecules, which insects detect using proteins encoded by multigenic families that emerged following land colonization by insect ancestors, namely the odorant-binding proteins (OBPs) and the odorant receptors (ORs). However, land-to-freshwater transitions occurred in many lineages within the insect tree of life. Whether chemosensory gene repertoires of aquatic insects remained essentially unchanged or underwent more or less drastic modifications to cope with physico-chemical constraints associated with life underwater remains virtually unknown. To address this issue, we sequenced and analyzed the transcriptome of chemosensory organs of the diving beetle Rhantus suturalis (Coleoptera, Dytiscidae). A reference transcriptome was assembled de novo using reads from five RNA-seq libraries (male and female antennae, male and female palps, and wing muscle). It contained 47,570 non-redundant unigenes encoding proteins of more than 50 amino acids. Within this reference transcriptome, we annotated sequences coding 53 OBPs, 48 ORs, 73 gustatory receptors (GRs), and 53 ionotropic receptors (IRs). Phylogenetic analyses notably revealed a large OBP gene expansion (35 paralogs in R. suturalis) as well as a more modest OR gene expansion (9 paralogs in R. suturalis) that may be specific to diving beetles. Interestingly, these duplicated genes tend to be expressed in palps rather than in antennae, suggesting a possible adaptation with respect to the land-to-water transition. This work provides a strong basis for further evolutionary and functional studies that will elucidate how insect chemosensory systems adapted to life underwater.


2021 ◽  
Vol 11 ◽  
Author(s):  
Paulina Maria Nawrocka ◽  
Paulina Galka-Marciniak ◽  
Martyna Olga Urbanek-Trzeciak ◽  
Ilamathi M-Thirusenthilarasan ◽  
Natalia Szostak ◽  
...  

Basal cell carcinoma (BCC) of the skin is the most common cancer in humans, characterized by the highest mutation rate among cancers, and is mostly driven by mutations in genes involved in the hedgehog pathway. To date, almost all BCC genetic studies have focused exclusively on protein-coding sequences; therefore, the impact of noncoding variants on the BCC genome is unrecognized. In this study, with the use of whole-exome sequencing of 27 tumor/normal pairs of BCC samples, we performed an analysis of somatic mutations in both protein-coding sequences and gene-associated noncoding regions, including 5’UTRs, 3’UTRs, and exon-adjacent intron sequences. Separately, in each region, we performed hotspot identification, mutation enrichment analysis, and cancer driver identification with OncodriveFML. Additionally, we performed a whole-genome copy number alteration analysis with GISTIC2. Of the >80,000 identified mutations, ~50% were localized in noncoding regions. The results of the analysis generally corroborated the previous findings regarding genes mutated in coding sequences, including PTCH1, TP53, and MYCN, but more importantly showed that mutations were also clustered in specific noncoding regions, including hotspots. Some of the genes specifically mutated in noncoding regions were identified as highly potent cancer drivers, of which BAD had a mutation hotspot in the 3’UTR, DHODH had a mutation hotspot in the Kozak sequence in the 5’UTR, and CHCHD2 frequently showed mutations in the 5’UTR. All of these genes are functionally implicated in cancer-related processes (e.g., apoptosis, mitochondrial metabolism, and de novo pyrimidine synthesis) or the pathogenesis of UV radiation-induced cancers. We also found that the identified BAD and CHCHD2 mutations frequently occur in melanoma but not in other cancers via The Cancer Genome Atlas analysis. Finally, we identified a frequent deletion of chr9q, encompassing PTCH1, and unreported frequent copy number gain of chr9p, encompassing the genes encoding the immune checkpoint ligands PD-L1 and PD-L2. In conclusion, this study is the first systematic analysis of coding and noncoding mutations in BCC and provides a strong basis for further analyses of the variants in BCC and cancer in general.


Author(s):  
Xinjun Liu ◽  
Peng Zhang ◽  
Shimul Kanti Nath ◽  
Shuai Li ◽  
Sanjoy Kumar Nandi ◽  
...  

Abstract Volatile memristors, or threshold switching devices, exhibit a diverse range of negative differential resistance (NDR) characteristics under current-controlled operation and understanding the origin of these responses is of great importance for exploring their potential as nano-scale oscillators for neuromorphic computing. Here we use a developed two-zone, parallel memristor model of NDR to undertake a systematic analysis of NDR modes in two-terminal metal-oxide-metal devices. The model assumes that the non-uniform current distribution associated with filamentary conduction can be represented by a high current density core and a lower current-density shell where the core is assumed to have a memristive response due to Poole-Frenkel conduction and the shell is represented by either a fixed resistor or a second memristive region. The detail analysis of the electrical circuits is undertaken using a lumped-element thermal model of the core-shell structure, and is shown to reproduce continuous and discontinuous NDR responses, as well as more complex compound behaviour. Finally, an interesting double-window oscillation behaviour is predicted and experimentally verified for a device with compound NDR behaviour. These results clearly identify the origin of different NDR responses and provide a strong basis for designing devices with complex NDR characteristics.


2021 ◽  
Author(s):  
Boas Pucker ◽  
Samuel F. Brockington

Here we respond to Zhou et al., 2020 'Combined Transcriptome and Metabolome analysis of Pitaya fruit unveiled the mechanisms underlying Peel and pulp color formation' published in BMC Genomics. Given the evolutionary conserved anthocyanin biosynthesis pathway in betalain-pigmented species, we are open to the idea that species with both anthocyanins and betalains might exist. However, in absence of LC-MS/MS spectra, apparent lack of biological replicates, and no comparison to authentic standards, the findings of Zhou et al., 2020 are not a strong basis to propose the presence of anthocyanins in betalain-pigmented pitaya. In addition, our re-analysis of the datasets indicates the misidentification of important genes and the omission of key anthocyanin synthesis genes ANS and DFR. Finally, our re-analysis of the RNA-Seq dataset reveals no correlation between anthocyanin biosynthesis gene expression and pigment status.


Agronomy ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2332
Author(s):  
Shenghong Zheng ◽  
Kang Ni ◽  
Lingfei Ji ◽  
Chenguang Zhao ◽  
Hongling Chai ◽  
...  

Crop coefficient (Kc) is one of the most significant parameters for crop water demand prediction and irrigation scheduling. However, there is a lack of knowledge about water vapor and Kc in tea plantation ecosystems. This study explored and determined the actual evapotranspiration (ETc) and Kc of two cultivars of tea (Camellia sinensis), clone variety Baiye1 (BY1) and Longjing43 (LJ43), based on lysimeter data. An estimation was made for both for ETc and adjusted ETc, and the corresponding Kc and adjusted Kc. The results showed that the adjusted ETc and Kc values revealed a minor fluctuation when compared to the ETc and Kc values during the experimental period, which indicated that the adjusted ETc and Kc values were more precise and practical to field conditions. The average adjusted Kc values were 0.71 (range of 0.43–1.02) for BY1 and 0.84 (range of 0.48–1.22) for LJ43. Additionally, heavy pruning can decrease ETc and Kc values, possibly due to the lower level of LAI after pruning. Moreover, it is clearly manifested that BY1 consistently had lower ETc and Kc values than those of LJ43 because of plant growth status differences between BY1 and LJ43. Overall, our study proposed a reliable reference of Kc in tea plantation, and illuminated the effects of pruning and plant growth differences on Kc, which could provide a strong basis for precise irrigation in tea plantations in a subtropical climate.


2021 ◽  
Vol 78 (4) ◽  
Author(s):  
Marco Ferretti

Abstract Key message Future international forest monitoring should build upon the existing pan-European programs. There is a renewed interest in the monitoring of European forests. Future monitoring systems should build upon existing international programs, making use of their strengths and solving their weaknesses. This approach will result into win–win solutions for both the existing and future systems. The UNECE ICP Forests has a number of characteristics that makes it a very good and strong basis for developing an advanced international forest monitoring system.


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