scholarly journals Feasibility of methylome analysis using small amounts of genomic DNA from formalin-fixed paraffin-embedded tissue

2018 ◽  
Vol 68 (11) ◽  
pp. 633-635 ◽  
Author(s):  
Kentaro Ohara ◽  
Eri Arai ◽  
Yoriko Takahashi ◽  
Yukihiro Fukamachi ◽  
Nanako Ito ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Formalin Fixed Paraffin ◽  
Methylome Analysis ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

scholarly journals Effective DNA/RNA Co-Extraction for Analysis of MicroRNAs, mRNAs, and Genomic DNA from Formalin-Fixed Paraffin-Embedded Specimens

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e34683 ◽  
Author(s):  
Adam Kotorashvili ◽  
Andrew Ramnauth ◽  
Christina Liu ◽  
Juan Lin ◽  
Kenny Ye ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Improved protocol for extraction of genomic DNA from formalin-fixed paraffin-embedded tissue samples without the use of xylene

2016 ◽  
Vol 54 (12) ◽  
Author(s):  
Arizumi Kikuchi ◽  
Takahiro Sawamura ◽  
Osami Daimaru ◽  
Masanobu Horie ◽  
Kazutoshi Sasaki ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Tissue Samples ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

KRAS mutation analysis in genomic DNA isolated from formalin-fixed paraffin-embedded ovarian tissue: evaluation of a strip-based reverse-hybridisation assay

2011 ◽  
Vol 64 (3) ◽  
pp. 252-256 ◽  
Author(s):  
Gernot Kriegshäuser ◽  
Veronika Auner ◽  
Eva Schuster ◽  
Barbara Holzer ◽  
Christian Oberkanins ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Ovarian Tissue ◽  
Analytical Sensitivity ◽  
Tissue Samples ◽  
Formalin Fixed Paraffin ◽  
Ffpe Samples ◽  
Formalin Fixed Paraffin Embedded ◽  
Sensitivity Specificity ◽  
Formalin Fixed ◽  
Reverse Hybridisation Assay

AimsTo evaluate a reverse-hybridisation assay (strip assay) designed for the sensitive detection of 10 mutations in codons 12 and 13 of the KRAS gene. The strip assay relies on mutant-enriched PCR followed by reverse-hybridisation of biotinylated amplification products to oligonucleotide probes immobilised as an array of parallel lines on nitrocellulose test strips.MethodsThe strip assay was used to analyse genomic DNA isolated from 120 formalin-fixed paraffin-embedded (FFPE) ovarian tissue samples. The samples were analysed in parallel using a biochip-based protocol (biochip assay) covering the same mutation spectrum, and results were compared with respect to sensitivity, specificity and operational input.ResultsThe strip assay identified 19 (16%) of 120 FFPE samples to carry a KRAS mutation; results were in agreement with those obtained by biochip hybridisation. Both assays had an analytical sensitivity of 1% when performed on FFPE-extracted DNA with approximately the same operational input needed for post-PCR processing. In contrast to the biochip assay, strip assay hybridisation may be automated to a large extent.ConclusionsThe strip assay is an accurate and sensitive tool for the low to medium throughput detection of KRAS mutation in genomic DNA isolated from FFPE tissue.

Genomic DNA extraction methods using formalin-fixed paraffin-embedded tissue

2015 ◽  
Vol 486 ◽  
pp. 17-23 ◽  
Author(s):  
Keerti Potluri ◽  
Ahmed Mahas ◽  
Michael N. Kent ◽  
Sameep Naik ◽  
Michael Markey
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Extraction Methods ◽  
Genomic Dna Extraction ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Dna Extraction Methods ◽  
Formalin Fixed

scholarly journals Evaluation of the genomic DNA extracted from formalin-fixed, paraffin-embedded oral samples archived for the past 40-years

2005 ◽  
Vol 41 (6) ◽  
Author(s):  
Tatiana Nayara Libório ◽  
Adriana Etges ◽  
Adriana da Costa Neves ◽  
Ricardo Alves Mesquita ◽  
Fábio Daumas Nunes
Keyword(s):  
Genomic Dna ◽  
The Past ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed
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