Plasmodiophora brassicae resting spore dynamics in clubroot resistant canola (Brassica napus ) cropping systems

2018 ◽  
Vol 68 (2) ◽  
pp. 399-408 ◽  
Author(s):  
T. W. Ernst ◽  
S. Kher ◽  
D. Stanton ◽  
D. C. Rennie ◽  
S. F. Hwang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Plasmodiophora Brassicae ◽  
Cropping Systems ◽  
Resting Spore

Influence of resistant cultivars and crop intervals on clubroot of canola

2019 ◽  
Vol 99 (6) ◽  
pp. 862-872 ◽  
Author(s):  
S.F. Hwang ◽  
H.U. Ahmed ◽  
Q. Zhou ◽  
H. Fu ◽  
G.D. Turnbull ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Yield ◽  
Plant Biomass ◽  
Plasmodiophora Brassicae ◽  
Disease Index ◽  
Yield Increase ◽  
Resting Spore ◽  
Important Constraint ◽  
Significant Yield ◽  
Very High

Clubroot, caused by Plasmodiophora brassicae, is an important constraint on canola (Brassica napus) production in Canada. Rotations of clubroot-resistant (CR) canola cultivars in various sequences and planting intervals between canola with non-host crops and fallow periods were evaluated to determine their effects on clubroot severity and P. brassicae resting spore populations under field and micro-plot conditions. Under micro-plot conditions, the rotation sequences including CR canola, continuous fallow, and the non-host barley reduced gall weight by 63%–100% and clubroot severity by 34%–100% compared with continuous planting of susceptible canola. No visible clubroot symptoms developed following continuous fallow or the non-host crop. Under field conditions, clubroot severity was very high (78% disease index) in the continuous susceptible canola sequence. Most of the CR canola rotation sequences significantly reduced clubroot severity by 12%–23%, but continuous fallow, continuous barley, and alternating the CR canola cultivars ‘45H29’ or ‘73-47’ with ‘TC72429-10’ reduced clubroot severity by 32%–36%. A comparison of intervals between canola crops and four cropping sequences (continuous susceptible canola, alternating canola with barley or pea, a 2-yr non-host interval between canola crops, and a 3-yr non-host interval between canola crops) was conducted over 5 yr. A 2- or 3-yr non-host interval improved plant height, plant biomass, and seed yield, and reduced gall mass, P. brassicae propagules in the soil, and clubroot severity. A significant yield increase of more than 3600% was observed in a 3-yr non-host interval.

Effects of root exudates and pH on Plasmodiophora brassicae resting spore germination and infection of canola (Brassica napus L.) root hairs

2013 ◽  
Vol 48 ◽  
pp. 16-23 ◽  
Author(s):  
A. Rashid ◽  
H.U. Ahmed ◽  
Q. Xiao ◽  
S.F. Hwang ◽  
S.E. Strelkov
Keyword(s):  
Brassica Napus ◽  
Root Exudates ◽  
Spore Germination ◽  
Plasmodiophora Brassicae ◽  
Root Hairs ◽  
Brassica Napus L ◽  
Resting Spore

scholarly journals Greenhouse Evaluation of Clubroot Resistant-Brassica napus cv. Mendel and Its Efficacy Concerning Virulence and Soil Inoculum Levels of Plasmodiophora brassicae

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 151 ◽  
Author(s):  
Nazanin Zamani-Noor ◽  
Imke Krohne ◽  
Birger Koopmann
Keyword(s):  
Brassica Napus ◽  
Plasmodiophora Brassicae ◽  
Root Weight ◽  
Susceptible Cultivar ◽  
Clubroot Resistance ◽  
Soil Inoculum ◽  
Resting Spore ◽  
Greenhouse Evaluation ◽  
Major Resistance Gene ◽  
Resting Spores

Clubroot resistance of oilseed rape (OSR) cultivars frequently relies on a major resistance gene originating from cv. Mendel. The efficacy of this resistance was studied in greenhouse experiments using two Plasmodiophora brassicae isolates, which were either virulent (P1(+)) or avirulent (P1) on Mendel. Seeds of clubroot-susceptible cultivar Visby and clubroot-resistant cultivar Mendel were sown in soil mixtures inoculated with different concentrations of resting spores (101, 103, 105, and 107 resting spores/g soil). Clubroot severity, plant height, shoot and root weight as well as resting spore propagation were assessed for each isolate and cultivar separately at four dates after sowing. The OSR cultivars behaved significantly different in the measured parameters. The threshold of inoculum density to cause disease depended strongly on the virulence of the pathogen and susceptibility of the host plant. In Visby grown in soil infested with P1, clubroot symptoms and increases in root weight and the number of propagated resting spores occurred at inoculum levels of 101 resting spores and higher, whereas Mendel was not affected in soils under the three lowest inoculum densities. In contrast, the P1(+) isolate led to earlier and more severe symptoms, heavier galls, and a significantly higher number of new resting spores in both cultivars.

Evaluation of Lime Products for Clubroot (Plasmodiophora brassicae) Management in Canola (Brassica napus) Cropping Systems

2021 ◽  
Author(s):  
Nicole M. Fox ◽  
Sheau-Fang Hwang ◽  
Victor P. Manolii ◽  
George Turnbull ◽  
Stephen E. Strelkov
Keyword(s):  
Brassica Napus ◽  
Plasmodiophora Brassicae ◽  
Cropping Systems

scholarly journals Clubroot Symptoms and Resting Spore Production in a Doubled Haploid Population of Oilseed Rape (Brassica napus) Are Controlled by Four Main QTLs

2020 ◽  
Vol 11 ◽  
Author(s):  
Andrea Botero-Ramírez ◽  
Anne Laperche ◽  
Solenn Guichard ◽  
Mélanie Jubault ◽  
Antoine Gravot ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Plasmodiophora Brassicae ◽  
Spore Production ◽  
Doubled Haploid Population ◽  
Resting Spore ◽  
Monogenic Resistance ◽  
Pathogen Fitness ◽  
Haploid Population

Clubroot, caused by Plasmodiophora brassicae Woronin, is one of the most important diseases of oilseed rape (Brassica napus L.). The rapid erosion of monogenic resistance in clubroot-resistant (CR) varieties underscores the need to diversify resistance sources controlling disease severity and traits related to pathogen fitness, such as resting spore production. The genetic control of disease index (DI) and resting spores per plant (RSP) was evaluated in a doubled haploid (DH) population consisting of 114 winter oilseed rape lines, obtained from the cross ‘Aviso’ × ‘Montego,’ inoculated with P. brassicae isolate “eH.” Linkage analysis allowed the identification of three quantitative trait loci (QTLs) controlling DI (PbBn_di_A02, PbBn_di_A04, and PbBn_di_C03). A significant decrease in DI was observed when combining effects of the three resistance alleles at these QTLs. Only one QTL, PbBn_rsp_C03, was found to control RSP, reducing resting spore production by 40%. PbBn_rsp_C03 partially overlapped with PbBn_di_C03 in a nucleotide-binding leucine-rich repeat (NLR) gene-containing region. Consideration of both DI and RSP in breeding for clubroot resistance is recommended for the long-term management of this disease.

Root Gall Formation, Resting Spore Isolation and High Molecular Weight DNA Extraction of Plasmodiophora brassicae

BIO-PROTOCOL ◽  
2018 ◽  
Vol 8 (11) ◽  
Author(s):  
Sara Mehrabi ◽  
Suzana Stjelja ◽  
Christina Dixelius
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Dna Extraction ◽  
Plasmodiophora Brassicae ◽  
Gall Formation ◽  
High Molecular Weight Dna ◽  
Resting Spore

scholarly journals Mapping of clubroot (Plasmodiophora brassicae) resistance in canola (Brassica napus)

2015 ◽  
Vol 65 (3) ◽  
pp. 435-440 ◽  
Author(s):  
H. Zhang ◽  
J. Feng ◽  
S.-F. Hwang ◽  
S. E. Strelkov ◽  
I. Falak ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Plasmodiophora Brassicae

scholarly journals Endosphere microbiome comparison between symptomatic and asymptomatic roots of Brassica napus infected with Plasmodiophora brassicae

PLoS ONE ◽  
2017 ◽  
Vol 12 (10) ◽  
pp. e0185907 ◽  
Author(s):  
Ying Zhao ◽  
Zhixiao Gao ◽  
Binnian Tian ◽  
Kai Bi ◽  
Tao Chen ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Plasmodiophora Brassicae

The life history of Plasmodiophora brassicae Woron

1972 ◽  
Vol 180 (1058) ◽  
pp. 103-112 ◽  
Keyword(s):  
Life History ◽  
Root Hair ◽  
Plasmodiophora Brassicae ◽  
Root Hairs ◽  
Secondary Phase ◽  
Tissue Cultures ◽  
Resting Spore ◽  
Resting Spores ◽  
Seedling Roots ◽  
History Of

Resting spore germination and the root hair stages of the life history of Plasmodiophora brassicae were studied in stained preparations of infected Brassica rapa seedling roots. Naked protoplasts, usually possessing two unequal flagella, were released from resting spores through a small circular pore. They penetrated the root hairs of B. rapa and there developed into plasmodia which, after becoming multinucleate, cleaved to form zoosporangia con­taining incipient zoospores. Biflagellate zoospores were released from root hair zoosporangia and fused in pairs, although karyogamy did not occur. The resulting binucleate zoospores infected the cortical dells of B. rapa to form binucleate plasmodia, the earliest stages of the secondary phase of the life history. These findings are discussed in relation to previous studies on the life history of P. brassicae in Brassica plants and in Brassica tissue cultures, and a new complete life history, including nuclear fusion in the secondary plasmodium, is suggested for the organism.

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