Quantitative trait loci conferring blast resistance in hexaploid wheat at adult plant stage

Powdery mildew, caused by Blumeria graminis f. sp. tritici, is a major disease to wheat (Triticum aestivum) worldwide. Use of adult-plant resistance (APR) is an effective method to develop wheat cultivars with durable resistance to powdery mildew. In the present study, 432 molecular markers were used to map quantitative trait loci (QTL) for APR to powdery mildew in a doubled haploid (DH) population with 107 lines derived from the cross Fukuho-komugi × Oligoculm. Field trials were conducted in Beijing and Anyang, China during 2003-2004 and 2004-2005 cropping seasons, respectively. The DH lines were planted in a randomized complete block design with three replicates. Artificial inoculation was carried out in Beijing with highly virulent isolate E20 of B. graminis f. sp. tritici and the powdery mildew severity on penultimate leaf was evaluated four times, and the maximum disease severity (MDS) on penultimate leaf was investigated in Anyang under natural inoculation in May 2004 and 2005. The heritability of resistance to powdery mildew for MDS in 2 years and two locations ranged from 0.82 to 0.93, while the heritability for area under the disease progress curve was between 0.84 and 0.91. With the method of composite interval mapping, four QTL for APR to powdery mildew were detected on chromosomes 1AS, 2BL, 4BL, and 7DS, explaining 5.7 to 26.6% of the phenotypic variance. Three QTL on chromosomes 1AS, 2BL, and 7DS were derived from the female, Fukuho-komugi, while the one on chromosome 4BL was from the male, Oligoculm. The QTL on chromosome 1AS showed high genetic effect on powdery mildew resistance, accounting for 19.5 to 26.6% of phenotypic variance across two environments. The QTL on 7DS associated with the locus Lr34/Yr18, flanked by microsatellite Xgwm295.1 and Ltn (leaf tip necrosis). These results will benefit for improving powdery mildew resistance in wheat breeding programs.

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Quantitative trait loci mapping of adult-plant resistance to leaf rust in a Fundulea 900 × ‘Thatcher’ wheat cross

Plant Breeding ◽

10.1111/pbr.12434 ◽

2016 ◽

Vol 136 (1) ◽

pp. 1-7 ◽

Cited By ~ 10

Author(s):

Peipei Zhang ◽

Aiyong Qi ◽

Yue Zhou ◽

Xianchun Xia ◽

Zhonghu He ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Leaf Rust ◽

Plant Resistance ◽

Quantitative Trait ◽

Adult Plant Resistance ◽

Adult Plant ◽

Quantitative Trait Loci Mapping ◽

Trait Loci ◽

Wheat Cross

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Quantitative Trait Loci for Adult-Plant Resistance to Mycosphaerella graminicola in Two Winter Wheat Populations

Phytopathology ◽

10.1094/phyto-08-10-0203 ◽

2011 ◽

Vol 101 (10) ◽

pp. 1209-1216 ◽

Cited By ~ 31

Author(s):

P. Risser ◽

E. Ebmeyer ◽

V. Korzun ◽

L. Hartl ◽

T. Miedaner

Keyword(s):

Quantitative Trait Loci ◽

Winter Wheat ◽

Plant Height ◽

Plant Resistance ◽

Quantitative Trait ◽

Adult Plant Resistance ◽

Heading Date ◽

Adult Plant ◽

Phenotypic Variance ◽

Trait Loci

Septoria tritici blotch (STB) is one of the most important leaf spot diseases in wheat worldwide. The goal of this study was to detect chromosomal regions for adult-plant resistance in large winter wheat populations to STB. Inoculation by two isolates with virulence to Stb6 and Stb15, both present in the parents, was performed and STB severity was visually scored plotwise as percent coverage of flag leaves with pycnidia-bearing lesions. ‘Florett’/‘Biscay’ and ‘Tuareg’/‘Biscay’, each comprising a cross of a resistant and a susceptible cultivar, with population sizes of 316 and 269 F7:8 recombinant inbred lines, respectively, were phenotyped across four and five environments and mapped with amplified fragment length polymorphism, diversity array technology, and simple sequence repeat markers covering polymorphic regions of ≈1,340 centimorgans. Phenotypic data revealed significant (P < 0.01) genotypic differentiation for STB, heading date, and plant height. Entry-mean heritabilities (h2) for STB were 0.73 for ‘Florett’/‘Biscay’ and 0.38 for ‘Tuareg’/‘Biscay’. All correlations between STB and heading date as well as between STB and plant height were low (r = –0.13 to –0.20). In quantitative trait loci (QTL) analysis, nine and six QTL were found for STB ratings explaining, together, 55 and 51% of phenotypic variation in ‘Florett’/‘Biscay’ and ‘Tuareg’/‘Biscay’, respectively. Genotype–environment and QTL–environment interactions had a large impact. Two major QTL were detected consistently across environments on chromosomes 3B and 6D from ‘Florett’ and chromosomes 4B and 6B from ‘Tuareg’, each explaining 12 to 17% of normalized adjusted phenotypic variance. These results indicate that adult-plant resistance to STB in both mapping populations was of a quantitative nature.

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Molecular Mapping and Analysis of an Excellent Quantitative Trait Loci Conferring Adult-Plant Resistance to Stripe Rust in Chinese Wheat Landrace Gaoxianguangtoumai

Frontiers in Plant Science ◽

10.3389/fpls.2021.756557 ◽

2021 ◽

Vol 12 ◽

Author(s):

Yuqi Wang ◽

Fengying Liang ◽

Fangnian Guan ◽

Fangjie Yao ◽

Li Long ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Stripe Rust ◽

Plant Resistance ◽

Quantitative Trait ◽

Adult Plant Resistance ◽

Puccinia Striiformis ◽

Adult Plant ◽

Wheat Landrace ◽

Trait Loci ◽

Chinese Wheat

The Chinese wheat landrace “Gaoxianguangtoumai” (GX) has exhibited a high level of adult-plant resistance (APR) to stripe rust in the field for more than a decade. To reveal the genetic background for APR to stripe rust in GX, a set of 249 F6:8 (F6, F7, and F8) recombinant inbred lines (RILs) was developed from a cross between GX and the susceptible cultivar “Taichung 29.” The parents and RILs were evaluated for disease severity at the adult-plant stage in the field by artificial inoculation with the currently predominant Chinese Puccinia striiformis f. sp. tritici races during three cropping seasons and genotyped using the Wheat 55K single-nucleotide polymorphism (SNP) array to construct a genetic map with 1,871 SNP markers finally. Two stable APR quantitative trait loci (QTL), QYr.GX-2AS and QYr.GX-7DS in GX, were detected on chromosomes 2AS and 7DS, which explained 15.5–27.0% and 11.5–13.5% of the total phenotypic variation, respectively. Compared with published Yr genes and QTL, QYr.GX-7DS and Yr18 may be the same, whereas QYr.GX-2AS is likely to be novel. Haplotype analysis revealed that QYr.GX-2AS is likely to be rare which presents in 5.3% of the 325 surveyed Chinese wheat landraces. By analyzing a heterogeneous inbred family (HIF) population from a residual heterozygous plant in an F8 generation of RIL, QYr.GX-2AS was further flanked by KP2A_36.85 and KP2A_38.22 with a physical distance of about 1.37Mb and co-segregated with the KP2A_37.09. Furthermore, three tightly linked Kompetitive allele-specific PCR (KASP) markers were highly polymorphic among 109 Chinese wheat cultivars. The results of this study can be used in wheat breeding for improving resistance to stripe rust.

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A Genome-Wide Meta-Analysis of Rice Blast Resistance Genes and Quantitative Trait Loci Provides New Insights into Partial and Complete Resistance

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-21-7-0859 ◽

2008 ◽

Vol 21 (7) ◽

pp. 859-868 ◽

Cited By ~ 216

Author(s):

Elsa Ballini ◽

Jean-Benoît Morel ◽

Gaétan Droc ◽

Adam Price ◽

Brigitte Courtois ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Resistance Genes ◽

Quantitative Trait ◽

Rice Blast ◽

Blast Resistance ◽

Durable Resistance ◽

Rice Genome ◽

Blast Disease ◽

Trait Loci ◽

Blast Resistance Genes

The completion of the genome sequences of both rice and Magnaporthe oryzae has strengthened the position of rice blast disease as a model to study plant–pathogen interactions in monocotyledons. Genetic studies of blast resistance in rice were established in Japan as early as 1917. Despite such long-term study, examples of cultivars with durable resistance are rare, partly due to our limited knowledge of resistance mechanisms. A rising number of blast resistance genes and quantitative trait loci (QTL) have been genetically described, and some have been characterized during the last 20 years. Using the rice genome sequence, can we now go a step further toward a better understanding of the genetics of blast resistance by combining all these results? Is such knowledge appropriate and sufficient to improve breeding for durable resistance? A review of bibliographic references identified 85 blast resistance genes and approximately 350 QTL, which we mapped on the rice genome. These data provide a useful update on blast resistance genes as well as new insights to help formulate hypotheses about the molecular function of blast QTL, with special emphasis on QTL for partial resistance. All these data are available from the OrygenesDB database.

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