Evaluation ofCoxiella burnetiiStatus in Dairy Cattle Herds with Bulk-tank Milk Positive by ELISA and PCR

2012 ◽  
Vol 61 (2) ◽  
pp. 163-168 ◽  
Author(s):  
A. Piñero ◽  
J. F. Barandika ◽  
A. Hurtado ◽  
A. L. García-Pérez
Keyword(s):  
Dairy Cattle ◽  
Bulk Tank Milk ◽  
Bulk Tank ◽  
Cattle Herds

Prevalence and risk factors for Coxiella burnetii (Q fever) in Dutch dairy cattle herds based on bulk tank milk testing

2014 ◽  
Vol 117 (1) ◽  
pp. 103-109 ◽  
Author(s):  
E. van Engelen ◽  
N. Schotten ◽  
B. Schimmer ◽  
J.L.A. Hautvast ◽  
G. van Schaik ◽  
...  
Keyword(s):  
Risk Factors ◽  
Dairy Cattle ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Bulk Tank Milk ◽  
Bulk Tank ◽  
Cattle Herds

scholarly journals Mapping geographical areas at risk for tick-borne encephalitis (TBE) by analysing bulk tank milk from Swedish dairy cattle herds for the presence of TBE virus–specific antibodies

2021 ◽  
Vol 63 (1) ◽  
Author(s):  
Gunilla Blomqvist ◽  
Katarina Näslund ◽  
Linda Svensson ◽  
Cécile Beck ◽  
Jean Francois Valarcher
Keyword(s):  
Dairy Cattle ◽  
Geographical Distribution ◽  
Serum Antibody ◽  
Bulk Tank Milk ◽  
Serological Examination ◽  
Tick Borne Encephalitis ◽  
Swedish Dairy ◽  
Bulk Tank ◽  
Cattle Herds ◽  
Antibody Levels

Abstract Background The vector-borne human viral zoonosis tick-borne encephalitis (TBE) is of growing concern in Sweden. The area where TBE is considered endemic has expanded, with an increasing geographical distribution of Ixodes ricinus as the tick vector and a rising number of reported TBE cases in humans. Efforts to map TBE risk areas have been carried out by sentinel monitoring, mainly based on individual sampling and analysis of wild and domestic animals, as well as ticks, for tick-borne encephalitis virus (TBEV). However, the interpretation of the geographical distribution has been hampered by the patchy and focal nature of TBEV occurrence. This study presents TBEV surveillance data based on antibody analysis of bulk tank milk collected from dairy herds located throughout Sweden before (May) and after (November) the vector season. A commercial TBEV antibody ELISA was modified and evaluated for use in this study. Results The initial comparative TBEV antibody analysis revealed a good correlation between milk and serum antibody levels from individually sampled cows. Also, the TBEV-antibody levels for the mean-herd serum showed good comparability with TBEV antibody levels from bulk tank milk, thus indicating good predictability of seroprevalence when analysing bulk tank milk from a herd. Analyses of bulk tank milk samples collected from 616 herds in May and 560 herds in November showed a geographical distribution of TBEV seropositive herds that was largely consistent with reported human TBE cases. A few TBEV-reactive herds were also found outside known locations of human TBE cases. Conclusion Serological examination of bulk tank milk from dairy cattle herds may be a useful sentinel surveillance method to identify geographical presence of TBEV. In contrast to individual sampling this method allows a large number of animals to be monitored. TBEV seropositive herds were mainly found in coastal areas of southern Sweden similar to human TBE cases. However, some antibody-reactive herds were found outside known TBE areas at the time of the study.

scholarly journals Prevalence of Staphylococcus aureus and of methicillin-resistant S. aureus clonal complexes in bulk tank milk from dairy cattle herds in Lombardy Region (Northern Italy)

2016 ◽  
Vol 144 (14) ◽  
pp. 3046-3051 ◽  
Author(s):  
C. CORTIMIGLIA ◽  
M. LUINI ◽  
V. BIANCHINI ◽  
L. MARZAGALLI ◽  
F. VEZZOLI ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cattle ◽  
Northern Italy ◽  
Bulk Tank Milk ◽  
Methicillin Resistant ◽  
Lombardy Region ◽  
Single Locus Variant ◽  
Bulk Tank ◽  
Cattle Herds ◽  
Mrsa Strains

SUMMARYStaphylococcus aureus is the most important causative agent of subclinical mastitis in cattle resulting in reduced milk production and quality. Methicillin-resistant S. aureus (MRSA) strains has a clear zoonotic relevance, especially in the case of occupational exposure. The aim of the study was to evaluate the prevalence of S. aureus and MRSA in bulk tank milk (BTM) from dairy cattle herds in the Lombardy Region (Northern Italy) and to identify the main MRSA circulating genotypes. MRSA strains were characterized by susceptibility testing, multi-locus sequence typing (MLST), spa typing and SCCmec typing. A total 844 BTM samples were analysed and S. aureus and MRSA were detected in 47·2% and 3·8% of dairy herds, respectively. MLST showed that the majority (28/32) of isolates belonged to the typical livestock-associated lineages: ST398, ST97 and ST1. Interestingly, in this study we report for the first time the new ST3211, a single locus variant of ST(CC)22, with the newly described 462 aroE allele. Our study indicates high diffusion of S. aureus mastitis and low, but not negligible, prevalence of MRSA in the considered area, suggesting the need for planning specific control programmes for bovine mastitis caused by S. aureus, especially when MRSA is implicated.

scholarly journals Prevalence of contagious and environmental mastitis-causing bacteria in bulk tank milk and its relationships with milking practices of dairy cattle herds in São Miguel Island (Azores)

2015 ◽  
Vol 48 (2) ◽  
pp. 451-459 ◽  
Author(s):  
Carla Azevedo ◽  
Diana Pacheco ◽  
Luísa Soares ◽  
Ricardo Romão ◽  
Mónica Moitoso ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Bulk Tank Milk ◽  
Bulk Tank ◽  
Cattle Herds

scholarly journals Short communication: Characterization of Staphylococcus aureus from bulk tank milk of dairy cattle in Lombardy (northern Italy)

2020 ◽  
Vol 103 (3) ◽  
pp. 2685-2692
Author(s):  
A. Gazzola ◽  
A.M. Maisano ◽  
V. Bianchini ◽  
F. Vezzoli ◽  
A. Romanò ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cattle ◽  
Short Communication ◽  
Northern Italy ◽  
Bulk Tank Milk ◽  
Bulk Tank

Relationship between occurrence of mastitis pathogens in dairy cattle herds and raw-milk indicators of hygienic-sanitary quality

2008 ◽  
Vol 75 (1) ◽  
pp. 121-127 ◽  
Author(s):  
Luís IM Souto ◽  
Clarice Y Minagawa ◽  
Evelise O Telles ◽  
Márcio A Garbuglio ◽  
Marcos Amaku ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Bacterial Growth ◽  
Negative Correlation ◽  
Raw Milk ◽  
Mammary Glands ◽  
Plate Counts ◽  
Bulk Tank ◽  
Cattle Herds ◽  
Mastitis Pathogens ◽  
Infectious Mastitis

Mastitis is an inflammation of the mammary glands and in most cases it is caused by the presence of microorganisms. High mastitis rates in dairy cattle herds can cause an increase in total microorganism counts of bulk tank milk. The present paper was aimed at verifying whether the occurrence of mastitis in dairy cattle herds is reflected in raw-milk indicators of hygienic-sanitary quality. To observe the correlation among the analysed variables, we performed a logarithmical transformation (log10) of different indicator counts of raw milk and compared them with the occurrence of mastitis in dairy cattle herds. Few correlations were observed among mastitis cases in dairy cattle herds and the raw-milk indicators of hygienic-sanitary quality. We observed a negative correlation between the log10 of mesophilic aerobic plate counts and psychotropic aerobic plate counts when compared with the occurrence of no bacterial growth. The log10 of thermophilic aerobic plate counts and yeasts and mould aerobic plate counts presented a positive correlation with the cases of infectious mastitis and mastitis caused by Staphylococcus spp.

Coxiella burnetiiin bulk tank milk of dairy cattle in south-west England: FIG 1:

2012 ◽  
Vol 171 (6) ◽  
pp. 156.2-156 ◽  
Author(s):  
G. E. Valergakis ◽  
C. Russell ◽  
R. Grogono-Thomas ◽  
M. C. Eisler ◽  
A. J. Bradley
Keyword(s):  
Dairy Cattle ◽  
Bulk Tank Milk ◽  
South West ◽  
Bulk Tank ◽  
South West England
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