scholarly journals Diagnostic specificity of the African swine fever virus antibody detection enzyme-linked immunosorbent assay in feral and domestic pigs in the United States

2017 ◽  
Vol 64 (6) ◽  
pp. 1665-1668 ◽  
Author(s):  
H. C. Bergeron ◽  
P. S. Glas ◽  
K. R. Schumann
Keyword(s):  
United States ◽  
Immunosorbent Assay ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
The United States ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diagnostic Specificity ◽  
Virus Antibody ◽  
Domestic Pigs

scholarly journals Techniques of blood sampling for detection of African swine fever virus in wild boar and domestic pigs in the field conditions

2021 ◽  
pp. 285-294
Author(s):  
A. S. Pershin ◽  
A. R. Shotin ◽  
E. O. Morozova ◽  
A. S. Igolkin ◽  
O. A. Manuylova ◽  
...  
Keyword(s):  
Wild Boar ◽  
Immunosorbent Assay ◽  
Filter Paper ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Blood Sampling ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunoperoxidase Technique ◽  
Domestic Pigs

It is thought that due to the high virulence of the African swine fever virus its circulation in the Russian Federation is accompanied by a low seroprevalence. However taking into account a long-term ASF unfavourable situation, the introduction of the virus into the wild boar population, and the occurrence of attenuated viral variants, the significance of serological testing aimed at the detection of viral antibodies is increasing. To collect field samples of biological material from animals for molecular genetic, virological, and serological tests, filter paper, as well as swabs, can be used. The specificity and sensitivity of enzyme-linked immunosorbent assay when testing blood absorbed by filter paper are worse than those shown when testing sera, but they allow effective detection of African swine fever virus antibodies. It was demonstrated that blood absorbed on filter paper can be used for the immunoblot analysis, but the optimum performance could be achieved when the immunoperoxidase technique in combination with samples, taken by swabs was used. When comparing results of enzyme-linked immunosorbent assay performed on sera collected from domestic pigs (infected with ASFV isolates Antonovo 07/14 and Sobinka 07/15), and blood from ear veins absorbed on filter paper the sensitivity was 88.9%, specificity – 90.6%. However, the use of the immunoperoxidase technique for testing blood from swabs showed 100% coincidence with ELISA, while testing of sera with immunoperoxidase technique was superior to ELISA in sensitivity. This means blood sampling using swabs may be recommended for tests after proper validation. This technique can be especially useful for collecting data about infected wild boars because effective eradication strategies are impossible without such data.

scholarly journals Characterization of Anti-p54 Monoclonal Antibodies and Their Potential Use for African Swine Fever Virus Diagnosis

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 178
Author(s):  
Weldu Tesfagaber ◽  
Lulu Wang ◽  
Ghebremedhin Tsegay ◽  
Yibrah Tekle Hagoss ◽  
Zhenjiang Zhang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Roc Analysis ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Antibody Detection ◽  
Effective Vaccine ◽  
Enzyme Linked Immunosorbent Assay

African swine fever (ASF) is a highly lethal hemorrhagic viral disease of domestic pigs caused by African swine fever virus (ASFV). Although a good advance has been made to understand the virus, a safe and effective vaccine against ASFV is still lacking and its eradication solely depends on its early and accurate diagnosis. Thus, improving the available diagnostic assays and adding some validated techniques are useful for a range of serological investigations. The aim of this study was to produce and characterize p54 monoclonal antibodies with an ultimate goal of developing a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) for ASFV antibody detection. Five monoclonal antibodies against p54 protein expressed in Escherichia coli was generated and their characterizations were investigated. Furthermore, a competitive enzyme-linked immunosorbent assay (cELISA) based on a monoclonal antibody designated as 2A7 was developed. To evaluate the performance of the assay, a total of 365 pig serum samples (178 negative and 187 positive samples) were tested and a receiver-operating characteristic (ROC) analysis was applied to determine the cut-off value. Based on the ROC analysis, the area under the curve (AUC) was 0.982 (95% confidence interval: 96.9% to 99.4%), besides a sensitivity of 92.5% and a specificity of 98.9% was achieved when the percent inhibition of 20% was selected as a threshold. Moreover, the result showed an excellent agreement when compared to other commercially available blocking ELISA (kappa value = 0.912) and showed no reaction to other swine pathogens. Overall, the newly developed cELISA method offers a promising approach for a rapid and convenient ASFV serodiagnosis, which could be used as alternative to other serological assays for screening possible ASFV infection.

scholarly journals Reviewing the Potential Vectors and Hosts of African Swine Fever Virus Transmission in the United States

2019 ◽  
Vol 19 (7) ◽  
pp. 512-524 ◽  
Author(s):  
Andrew J. Golnar ◽  
Estelle Martin ◽  
Jillian D. Wormington ◽  
Rebekah C. Kading ◽  
Pete D. Teel ◽  
...  
Keyword(s):  
United States ◽  
African Swine Fever Virus ◽  
Virus Transmission ◽  
African Swine Fever ◽  
The United States ◽  
Fever Virus

Alternative sampling strategies for passive classical and African swine fever surveillance in wild boar – Extension towards African swine fever virus antibody detection

2014 ◽  
Vol 174 (3-4) ◽  
pp. 607-608 ◽  
Author(s):  
Sandra Blome ◽  
Katja V. Goller ◽  
Anja Petrov ◽  
Carolin Dräger ◽  
Jana Pietschmann ◽  
...  
Keyword(s):  
Wild Boar ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Antibody Detection ◽  
Sampling Strategies ◽  
Virus Antibody

scholarly journals A solid-phase enzyme linked immunosorbent assay for the detection of African swine fever virus antigen and antibody

1979 ◽  
Vol 83 (2) ◽  
pp. 363-370 ◽  
Author(s):  
R. C. Wardley ◽  
E. M. E. Abu Elzein ◽  
J. R. Crowther ◽  
P. J. Wilkinson
Keyword(s):  
Immunosorbent Assay ◽  
Solid Phase ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Virus Antigen ◽  
Fever Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diagnostic Use

summaryA solid-phase enzyme-linked immunosorbent assay was developed to measure both African swine fever virus (ASFV) antigen and antibody. Experiments showed it to be reproducible and able to detect limiting antigen concentrations of 50–500 HAD50/ml. The assay was more sensitive than those used at present to detect ASFV antibody and it is suggested that it might be of great diagnostic use in countries where African swine fever has recently appeared.

Highly specific confirmatory Western blot test for African swine fever virus antibody detection using the recombinant virus protein p54

1995 ◽  
Vol 52 (1-2) ◽  
pp. 111-119 ◽  
Author(s):  
C. Alcaraz ◽  
F. Rodriguez ◽  
J.M. Oviedo ◽  
A. Eiras ◽  
M. De Diego ◽  
...  
Keyword(s):  
Western Blot ◽  
Recombinant Virus ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Antibody Detection ◽  
Virus Protein ◽  
Virus Antibody ◽  
Western Blot Test ◽  
Confirmatory Western Blot

scholarly journals Influenza A plasma and serum virus antibody detection comparison in dogs using blocking enzyme-linked immunosorbent assay

2015 ◽  
Vol 8 (5) ◽  
pp. 580-583 ◽  
Author(s):  
H. T. Lin ◽  
C. H. Hsu ◽  
H. J. Tsai ◽  
C. H. Lin ◽  
P. Y. Lo ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Influenza A ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Antibody

scholarly journals Rethinking the uncertainty of African swine fever virus contamination in feed ingredients and risk of introduction into the United States

2021 ◽  
Author(s):  
Rachel A. Schambow ◽  
Fernando Sampedro ◽  
Pedro E. Urriola ◽  
Jennifer L. G. Ligt ◽  
Andres Perez ◽  
...  
Keyword(s):  
United States ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
The United States ◽  
Fever Virus ◽  
Feed Ingredients ◽  
Virus Contamination

scholarly journals Risk of African Swine Fever Virus Sylvatic Establishment and Spillover to Domestic Swine in the United States

2019 ◽  
Vol 19 (7) ◽  
pp. 506-511 ◽  
Author(s):  
Jillian D. Wormington ◽  
Andrew Golnar ◽  
Karen C. Poh ◽  
Rebekah C. Kading ◽  
Estelle Martin ◽  
...  
Keyword(s):  
United States ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
The United States ◽  
Fever Virus ◽  
Domestic Swine

scholarly journals Evaluation of a Diagnostic Algorithm Using Immunoglobulin M Enzyme-Linked Immunosorbent Assay To Differentiate Human West Nile Virus and St. Louis Encephalitis Virus Infections during the 2002 West Nile Virus Epidemic in the United States

2004 ◽  
Vol 11 (6) ◽  
pp. 1130-1133 ◽  
Author(s):  
Denise A. Martin ◽  
Amanda Noga ◽  
Olga Kosoy ◽  
Alison J. Johnson ◽  
Lyle R. Petersen ◽  
...  
Keyword(s):  
United States ◽  
West Nile Virus ◽  
Immunosorbent Assay ◽  
Diagnostic Algorithm ◽  
The United States ◽  
Encephalitis Virus ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Infections ◽  
West Nile

ABSTRACT A diagnostic algorithm was developed to differentiate between human infections of West Nile virus (WNV) and St. Louis encephalitis virus (SLEV) using positive-to-negative (P/N) ratios derived from the immunoglobulin M capture enzyme-linked immunosorbent assay (MAC-ELISA). To validate this algorithm, we tested 1,418 serum and cerebrospinal fluid (CSF) samples from confirmed WNV and SLEV infections collected during the WNV epidemic of 2002 in the United States. WNV P/N-to-SLEV P/N ratios (W/S ratios) were calculated and used to identify the infecting virus. These results were compared to results from the plaque reduction neutralization test (PRNT), which is currently the standard assay used to discriminate between closely related flavivirus infections. If the W/S ratio was ≥1, the predictive value positive (PNP) for WNV was 97.8%, where 95% of flavivirus cases were due to WNV infection and only 3.7% of specimens would require PRNT to differentiate WNV from SLEV infection. Use of the W/S ratio as part of the testing algorithm to interpret MAC-ELISA results generates reportable probable cases quickly, alleviating the need for PRNT in most instances.

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