H5N1 avian influenza virus without 80–84 amino acid deletion at the NS1 protein hijacks the innate immune system of dendritic cells for an enhanced mammalian pathogenicity

2020 ◽  
Author(s):  
Sujuan Chen ◽  
Xinyu Miao ◽  
Dandan Huangfu ◽  
Xinyi Zhao ◽  
Minxia Zhang ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Influenza Virus ◽  
Immune System ◽  
Amino Acid ◽  
Innate Immune System ◽  
Innate Immune ◽  
Ns1 Protein ◽  
Amino Acid Deletion ◽  
H5n1 Avian Influenza Virus ◽  
H5n1 Avian Influenza

Production and Characterization of Monoclonal Antibodies Against NS1 Protein of H5N1 Avian Influenza Virus

Hybridoma ◽  
2010 ◽  
Vol 29 (2) ◽  
pp. 183-186 ◽  
Author(s):  
Mohita Rai ◽  
Sandeep Bhatia ◽  
Y.P.S. Malik ◽  
S.C. Dubey
Keyword(s):  
Influenza Virus ◽  
Monoclonal Antibodies ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Ns1 Protein ◽  
H5n1 Avian Influenza Virus ◽  
H5n1 Avian Influenza

scholarly journals The innate immune system in SLE: type I interferons and dendritic cells

Lupus ◽  
2008 ◽  
Vol 17 (5) ◽  
pp. 394-399 ◽  
Author(s):  
L Rönnblom ◽  
V Pascual
Keyword(s):  
Dendritic Cells ◽  
Immune System ◽  
Innate Immune System ◽  
Innate Immune ◽  
Type I Interferons ◽  
Type I

scholarly journals Receptor specificity of the influenza virus hemagglutinin modulates sensitivity to soluble collectins of the innate immune system and virulence in mice

Virology ◽  
2011 ◽  
Vol 413 (1) ◽  
pp. 128-138 ◽  
Author(s):  
Michelle D. Tate ◽  
Andrew G. Brooks ◽  
Patrick C. Reading
Keyword(s):  
Influenza Virus ◽  
Immune System ◽  
Innate Immune System ◽  
Innate Immune ◽  
Receptor Specificity ◽  
Influenza Virus Hemagglutinin

scholarly journals Regulation of the innate immune system by autophagy: monocytes, macrophages, dendritic cells and antigen presentation

2019 ◽  
Vol 26 (4) ◽  
pp. 715-727 ◽  
Author(s):  
Nina Germic ◽  
Ziva Frangez ◽  
Shida Yousefi ◽  
Hans-Uwe Simon
Keyword(s):  
Dendritic Cells ◽  
Immune System ◽  
Antigen Presentation ◽  
Innate Immune System ◽  
Innate Immune

Innate Immune System Modulation During Aging: Contributions of Macrophages and Dendritic Cells

2010 ◽  
Vol 6 (4) ◽  
pp. 329-338 ◽  
Author(s):  
Belkys A. Maletto ◽  
Gabriel Moron ◽  
Maria Cristina Pistoresi-Palencia
Keyword(s):  
Dendritic Cells ◽  
Immune System ◽  
Innate Immune System ◽  
Innate Immune ◽  
Immune System Modulation

scholarly journals NS1 Protein Amino Acid Changes D189N and V194I Affect Interferon Responses, Thermosensitivity, and Virulence of Circulating H3N2 Human Influenza A Viruses

2016 ◽  
Vol 91 (5) ◽  
Author(s):  
Aitor Nogales ◽  
Luis Martinez-Sobrido ◽  
David J. Topham ◽  
Marta L. DeDiego
Keyword(s):  
Influenza Virus ◽  
Amino Acid ◽  
Immune Responses ◽  
Influenza A ◽  
Innate Immune ◽  
Temperature Sensitive ◽  
Ns1 Protein ◽  
Innate Immune Responses ◽  
Influenza A Viruses ◽  
Virus Surveillance

ABSTRACT Influenza virus NS1 protein is a nonstructural, multifunctional protein that counteracts host innate immune responses, modulating virus pathogenesis. NS1 protein variability in subjects infected with H3N2 influenza A viruses (IAVs) during the 2010/2011 season was analyzed, and amino acid changes in residues 86, 189, and 194 were found. The consequences of these mutations for the NS1-mediated inhibition of IFN responses and the pathogenesis of the virus were evaluated, showing that NS1 mutations D189N and V194I impaired the ability of the NS1 protein to inhibit general gene expression, most probably because these mutations decreased the binding of NS1 to the cleavage and polyadenylation specificity factor 30 (CPSF30). A recombinant A/Puerto Rico/8/34 (PR8) H1N1 virus encoding the H3N2 NS1-D189N protein was slightly attenuated, whereas the virus encoding the H3N2 NS1-V194I protein was further attenuated in mice. The higher attenuation of this virus could not be explained by differences in the ability of the two NS1 proteins to counteract host innate immune responses, indicating that another factor must be responsible. In fact, we showed that the virus encoding the H3N2 NS1-V194I protein demonstrated a temperature-sensitive (ts) phenotype, providing a most likely explanation for the stronger attenuation observed. As far as we know, this is the first description of a mutation in NS1 residue 194 conferring a ts phenotype. These studies are relevant in order to identify new residues important for NS1 functions and in human influenza virus surveillance to assess mutations affecting the pathogenicity of circulating viruses. IMPORTANCE Influenza viral infections represent a serious public health problem, with influenza virus causing a contagious respiratory disease that is most effectively prevented through vaccination. The multifunctional nonstructural protein 1 (NS1) is the main viral factor counteracting the host antiviral response. Therefore, influenza virus surveillance to identify new mutations in the NS1 protein affecting the pathogenicity of the circulating viruses is highly important. In this work, we evaluated amino acid variability in the NS1 proteins from H3N2 human seasonal viruses and the effect of the mutations on innate immune responses and virus pathogenesis. NS1 mutations D189N and V194I impaired the ability of the NS1 protein to inhibit general gene expression, and recombinant viruses harboring these mutations were attenuated in a mouse model of influenza infection. Interestingly, a virus encoding the H3N2 NS1-V194I protein demonstrated a temperature-sensitive phenotype, further attenuating the virus in vivo.

The role of dendritic cells in the innate immune system

2000 ◽  
Vol 2 (3) ◽  
pp. 257-272 ◽  
Author(s):  
Georgina J Clark ◽  
Nicola Angel ◽  
Masato Kato ◽  
J.Alejandro López ◽  
Kelli MacDonald ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Immune System ◽  
Innate Immune System ◽  
Innate Immune

W1786 Transfer of Dexamathasone Treated Dendritic Cells (Ddc) Prevent TNBS Colitis by Enhancing the Innate Immune System

2010 ◽  
Vol 138 (5) ◽  
pp. S-740
Author(s):  
André Z. Leite ◽  
Renata R. Polombo ◽  
Carmen Lucia Ortiz-Agostinho ◽  
Iêda Nishitokukado ◽  
Cyro A. Brito ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Immune System ◽  
Innate Immune System ◽  
Innate Immune ◽  
Tnbs Colitis
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