Sources and prevention of graft infection during long term ex situ liver perfusion

ABSTRACTWe have demonstrated earlier the useful performance of our PSI radiation-grafted membranes in terms of the current-voltage characteristics of 30 cm2 active area fuel cells containing these membranes and their long-term testing over 6,000 h at 60 °C. We report here on testing of PSI radiation-grafted membranes in these fuel cells at 80 °C and in short stacks comprised of two or four 100 cm2 active area cells. The in-situ degradation of membranes has been investigated by characterizing membranes both before testing in fuel cells and post-mortem after testing in fuel cells. Characterization was accomplished by means of ion-exchange capacity and infrared and Raman spectroscopic measurements. In addition, a rapid screening method for our ex-situ testing of the oxidative stability of proton-conducting membranes was developed in this work. Comparison of the initial screening test results concerning the oxidative stability of some perfluorinated, partially-fluorinated, and non-fluorinated membranes compare well qualitatively with the relative stability of these same membranes during their long-term testing in fuel cells.

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The National Tree Seed Centre Celebrates 40 Years

The Forestry Chronicle ◽

10.5558/tfc83719-5 ◽

2007 ◽

Vol 83 (5) ◽

pp. 719-722

Author(s):

J D Simpson ◽

B S.P. Wang

Keyword(s):

Seed Storage ◽

Ex Situ ◽

Future Research ◽

Gene Conservation ◽

Forest Land ◽

Seed Physiology ◽

Technology Research Group ◽

Insect Attack ◽

Tree Seed

The National Tree Seed Centre has been providing seed of known origin and quality for research for 40 years. Seed is also stored for long-term gene conservation purposes to provide a source of germplasm for future research and restoration. This is particularly important for species facing such threats as insect attack, disease, climate change, or conversion of forest land to non-forest uses. The Centre's inventory focuses on native tree and shrub species, striving to store samples from throughout their ranges. Over 26 000 seed samples have been sent to researchers in 65 countries, 70% of these samples being distributed within Canada. Seed research has always been a component of the Seed Centre's program. One notable accomplishment is the development of the Petawawa Germination Box. The Seed Centre participates in and contributes to activities of the Association of Official Seed Analysts, the International Seed Testing Association, and the IUFRO Seed Physiology and Technology Research Group. Key words: collection, dormancy, ex situ gene conservation, germination, research, seed, storage

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Long-term Successful Antibiotic Therapy for Recurrent Aortic Graft Infection

European Journal of Case Reports in Internal Medicine ◽

10.12890/2018_000913 ◽

2018 ◽

pp. 1

Author(s):

Liana Dykman ◽

Leore Cohen ◽

Micha Rapoport

Keyword(s):

Antibiotic Therapy ◽

Graft Infection ◽

Aortic Graft ◽

Aortic Graft Infection

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Long-term effects of a chemical decontamination procedure on the corrosion state of the heat exchanger tubes of steam generators

Nova Biotechnologica et Chimica ◽

10.36547/nbc.1362 ◽

2022 ◽

Vol 7 (1) ◽

pp. 77-83

Author(s):

Andrea Szabó Nagy ◽

Kálmán Varga ◽

Bernadett Baja ◽

Zoltán Németh ◽

Desző Oravetz ◽

...

Keyword(s):

Stainless Steel ◽

Heat Exchanger ◽

Oxide Layer ◽

In Situ Monitoring ◽

Ex Situ ◽

General Corrosion ◽

Long Term Effects ◽

Steam Generators ◽

Corrosion State

Our previous studies have revealed that a ”hybrid” structure of the amorphous and crystalline phases is formed in the outermost surface region of the austenitic stainless steel tubes of steam generators (SGs) as an undesired consequence of the industrial application of the AP-CITROX (AP: alkaline permanganate; CITROX: citric and oxalic acid) decontamination technology. The formation of this mobile oxide-layer increased the amount of the corrosion products in the primary circuit significantly, resulting in magnetite deposition on fuel assemblies. Owing to the fact that there is no investigation method available for the in-situ monitoring of the inner surfaces of heat exchanger tubes, a research project based on sampling as well as on ex-situ electrochemical and surface analytical measurements was elaborated. Within the frame of this project, comprehensive investigation of the general corrosion state and metallographic features of 36 stainless steel specimens, cut out from various locations of the 21 steam generators of the Paks NPP in the time period of 2000-2007 has been performed. The present work gives a brief overview on the general corrosion state of the heat exchanger tubes of SGs, concerning the long-term effects of the AP-CITROX procedure on the chemical composition and structure of the protective oxide-layer.

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Improved Conservation of Coffee (Coffea arabica L.) Germplasm via Micropropagation and Cryopreservation

Agronomy ◽

10.3390/agronomy11091861 ◽

2021 ◽

Vol 11 (9) ◽

pp. 1861

Author(s):

Yanelis Castilla Valdés ◽

Mukund R. Shukla ◽

María Esther González Vega ◽

Praveen K. Saxena

Keyword(s):

Coffea Arabica ◽

Plant Genetic Resources ◽

Ex Situ ◽

Zygotic Embryos ◽

Agricultural Crop ◽

Temporary Immersion ◽

Droplet Vitrification ◽

Coffea Arabica L

Coffee (Coffea spp.) is an important tropical agricultural crop that has significant economic and social importance in the world. The ex situ conservation of plant genetic resources through seeds is not feasible due to the sensitivity of coffee seed to desiccation and low temperatures. The cryopreservation of zygotic embryos may allow for an efficient and long-term storage of coffee germplasm. This study describes the cryopreservation methods for conserving zygotic embryos of Coffea arabica L. for the long-term conservation of currently available germplasm. Zygotic embryos were successfully cryopreserved in liquid nitrogen at −196 °C under controlled environmental conditions with either droplet-vitrification or encapsulation–vitrification protocols without dehydration. Zygotic embryos had the highest regrowth (100%) following droplet-vitrification cryopreservation using the Plant Vitrification Solution 3 (PVS3) for 40 min at 23 °C. In the case of encapsulation–vitrification using PVS3 for 40 min at 23 °C, the embryo regeneration response was 78%. Plantlets were recovered following shoot multiplication using a temporary immersion system (TIS) and in vitro rooting. The prolific rooting of shoots was observed after 4 weeks of culture in the liquid medium with plugs made of the inert substrate Oasis® In vitro Express (IVE) compared to the semi-solid medium. The successful cryopreservation of coffee zygotic embryos using droplet vitrification and encapsulation–vitrification followed by micropropagation in temporary immersion culture system has not been reported earlier and together these technologies are anticipated to further facilitate the initiatives for the conservation and distribution of coffee germplasm.

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Propagation of Threatened Nepenthes khasiana: Methods and Precautions

Notulae Scientia Biologicae ◽

10.15835/nsb739639 ◽

2015 ◽

Vol 7 (3) ◽

pp. 313-315

Author(s):

Jibankumar S. KHURAIJAM ◽

Rup K. ROY

Keyword(s):

Plant Species ◽

Threatened Species ◽

Ex Situ Conservation ◽

Ex Situ ◽

In The Wild ◽

Propagation Technique ◽

Subsequent Propagation ◽

Nepenthes Khasiana

Ex-situ conservation is an important key in the management of rare, endangered and threatened (RET) plant species and its effectiveness depends on several factors. Maintenance of viable germplasm and its subsequent propagation plays an important role in long term conservation of many RET species. Nepenthes khasiana is a rare and gravely threatened species in the wild due to over-collection and other threats. The species needs urgent in-situ and ex-situ conservation. Development of easy to propagate techniques would pave faster multiplication for its use of educational, medicinal and horticultural purpose. In the present paper, successful propagation technique of Nepenthes khasiana through seeds is demonstrated along with detailed information on precautions to be taken during the adoption of the techniques.

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Determination of Minimal Hemoglobin Level Necessary for Normothermic Porcine Ex Situ Liver Perfusion

Transplantation Journal ◽

10.1097/tp.0000000000002272 ◽

2018 ◽

Vol 102 (8) ◽

pp. 1284-1292 ◽

Cited By ~ 3

Author(s):

Mariusz Bral ◽

Boris Gala-Lopez ◽

Aducio Thiesen ◽

Sanaz Hatami ◽

David L. Bigam ◽

...

Keyword(s):

Liver Perfusion ◽

Hemoglobin Level ◽

Ex Situ

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Bile formation in long-term (1 week), ex situ perfused livers: Analysis and commentary

Surgery ◽

10.1016/j.surg.2020.10.014 ◽

2020 ◽

Author(s):

Guergana G. Panayotova ◽

Keri E. Lunsford ◽

James V. Guarrera

Keyword(s):

Ex Situ ◽

Bile Formation

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The decline and conservation management of the threatened endemic palms of the Mascarene Islands

Oryx ◽

10.1017/s0030605302000091 ◽

2002 ◽

Vol 36 (1) ◽

pp. 56-65 ◽

Cited By ~ 16

Author(s):

Mike Maunder ◽

Wayne Page ◽

John Mauremootoo ◽

Richard Payendee ◽

Yousoof Mungroo ◽

...

Keyword(s):

Large Scale ◽

Conservation Management ◽

Conservation Status ◽

Iucn Red List ◽

Ex Situ ◽

Population Fragmentation ◽

Wild Populations ◽

Mascarene Islands

Abstract The conservation status of the five genera and 11 species of palm endemic to the Mascarene Islands (Mauritius, La Réunion and Rodriques) are reviewed. All species are threatened with extinction; nine taxa are classified as Critically Endangered and four as Endangered on the 2000 IUCN Red List. Two taxa survive as single wild specimens (Hyophorbe amaricaulis and Dictyosperma album var. conjugatum); an additional seven taxa have wild populations of 100 or fewer. Although the historical phase of large-scale forest clearance has passed, the remaining palm populations in the Mascarenes are under threat from the effects of population fragmentation, invasive plants and animals, and high levels of seed predation that prevent natural regeneration. The advantages of in situ management for the recovery of these palm populations are discussed. Without a long-term conservation programme, utilising both in situ and ex situ management, extinction of wild populations will occur.

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