Treatment outcomes and prognostic factors of feline splenic mast cell tumors: A multi-institutional retrospective study of 64 cases

In this study, age, sex, recurrence, metastasis, death rate, and histologic patterns were in agreement with those of previous reports on canine mast cell tumors. Histologic grading, mitotic index, chromosome nucleolar organizer regions stained with silver (AgNORs), and anti-proliferating cell nuclear antigen (PCNA) were evaluated as indicators of prognosis. Histologic grading, AgNORs estimated in 100 cells, and PCNA-labeled fraction estimated in five high power fields (HPFs) were significantly different between recurring and nonrecurring tumors. Those prognostic factors were also significantly different between tumors that metastasized and those that did not. The survival time was lower in dogs with mast cell tumors with histologic grade 3 (Patnaik's), AgNOR counts higher than 2.25, and PCNA count in five HPFs higher than 261. The significance of these factors as markers for prognosis determined by logistic regression analysis differed with the time period considered. By combining the three most significant prognostic factors in a prognostic index, three models were obtained to determine the probability of nonrecurrence at 3, 6, and 9 months after surgery. The models were accurate in the prediction of the outcome of up to 80% of mast cell tumors. The use of these models provides a less subjective means of prognosticating mast cell tumors than the use of any one component alone.

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Prognosis Following Surgical Excision of Canine Cutaneous Mast Cell Tumors With Histopathologically Tumor-Free Versus Nontumor-Free Margins: A Retrospective Study of 31 Cases

Journal of the American Animal Hospital Association ◽

10.5326/0380458 ◽

2002 ◽

Vol 38 (5) ◽

pp. 458-466 ◽

Cited By ~ 71

Author(s):

Gina M. Michels ◽

Deborah W. Knapp ◽

Dennis B. DeNicola ◽

Nita Glickman ◽

Patty Bonney

Keyword(s):

Mast Cell ◽

Retrospective Study ◽

Small Sample Size ◽

Surgical Excision ◽

Local Relapse ◽

Small Sample ◽

Mast Cell Tumors ◽

Statistical Support ◽

Cutaneous Mast Cell ◽

Tumor Related Death

The purpose of this study was to determine if the presence of histopathologically tumor-free versus nontumor-free margins was prognostic for relapse or tumor-related death in dogs following surgical excision of single or multiple cutaneous mast cell tumors confined to the skin without evidence of metastasis to lymph nodes or other noncutaneous sites. Differences in tumor-related death or frequency of relapse between the two groups were not significant. Failure to achieve histopathological tumor-free margins frequently did not lead to local relapse. All tumor-related deaths occurred following local relapse. The lack of statistical support for an association between prognosis and histopathological tumor-free versus nontumor-free margins may be a result of small sample size.

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Clinical prognostic factors and treatment outcomes in patients with small cell carcinoma of the cervix: A single institution-based retrospective study

Oncology Journal of India ◽

10.4103/oji.oji_18_20 ◽

2020 ◽

Vol 4 (2) ◽

pp. 54

Author(s):

Niketa Thakur ◽

Sonal Patel

Keyword(s):

Prognostic Factors ◽

Retrospective Study ◽

Cell Carcinoma ◽

Treatment Outcomes ◽

Small Cell Carcinoma ◽

Small Cell ◽

Single Institution

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Prognostic Factors and Treatment Outcomes of Patients with Uterine Leiomyosarcoma: A Retrospective Study of Anatolian Society of Medical Oncology

Annals of Oncology ◽

10.1016/s0923-7534(20)33574-2 ◽

2012 ◽

Vol 23 ◽

pp. ix332-ix333

Author(s):

A. Durnali ◽

S. Tokluog˘ lu ◽

N. Ozdemir ◽

M. I˙nanc ◽

N. Alkis ◽

...

Keyword(s):

Prognostic Factors ◽

Retrospective Study ◽

Treatment Outcomes ◽

Medical Oncology ◽

Uterine Leiomyosarcoma

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Use of different fixation times and application of two immunohistochemical methods for detection of KIT and Ki67 proteins in canine cutaneous mast cell tumors

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-5843 ◽

2019 ◽

Vol 39 (1) ◽

pp. 52-60

Author(s):

Alex dos Santos ◽

Harlan H.L. Nascimento ◽

Mariana M. Flores ◽

Glaucia D. Kommers

Keyword(s):

Mast Cell ◽

Retrospective Study ◽

Prospective Study ◽

Biological Behavior ◽

Growth Fraction ◽

Fixation Time ◽

Mast Cell Tumors ◽

Grading Systems ◽

High Prevalence ◽

Cutaneous Mast Cell

ABSTRACT: Due to the high prevalence of mast cell tumors (MCTs) in the diagnostic routine, several factors, especially prognostic, have been sought to determine the biological behavior of these neoplasms. Immunohistochemistry (IHC) is one of the main tools utilized to biologically differentiate more aggressive tumors from less aggressive ones. However, some immunostainings are influenced by formalin fixation, interfering with the results. This is both a retrospective and prospective study of MCTs diagnosed in laboratory routine. A total of 25 samples, without knowledge about fixation time, were analyzed in the retrospective study, whereas 12 samples, with known fixation times, were assessed in the prospective study. Two histologic grading systems (Patnaik and Kiupel), special staining of toluidine blue, and IHC for KIT and Ki67 proteins were applied in both studies. Additionally, two amplification systems (biotinylated and non-biotinylated) for Ki67 protein and counting of the argyrophilic nucleolar organizing regions (AgNOR method) were tested in the prospective study. In the retrospective study, greater agreement between the evaluating pathologists was observed when the Kiupel system was used. IHC staining for KIT protein was effective in both studies, regardless of fixation time. IHC staining for Ki67 protein was highly sensitive to formaldehyde, and staining failure was observed in 56% of the cases in the retrospective study. In the prospective study, samples fixed for longer than 24 hours showed a reduction in the number of stained cells (altering the determination of the cell growth fraction) or showed absence of IHC staining in both amplification systems. The use of the AgNOR method to evaluate the rate of cell proliferation may be an alternative when the fixation time of the neoplasm is unknown or longer than 24 hours.

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