Characterisation of the serum cytokine profile in feline atopic skin syndrome

Tay-Sachs disease (TSD) is a progressive neurodegenerative disorder that occurs due to a deficiency of a β hexosaminidase A (HexA) enzyme, resulting in the accumulation of GM2 gangliosides. In this work, we analyzed the effect of umbilical cord blood cell transplantation (UCBCT) and curcumin administration on the course of the disease in a patient with adult TSD. The patient’s serum cytokine profile was determined using multiplex analysis. The level of GM2 gangliosides in plasma was determined using mass spectrometry. The enzymatic activity of HexA in the plasma of the patient was assessed using a fluorescent substrate assay. The HexA α-subunit (HexA) concentration was determined using ELISA. It was shown that both UCBCT and curcumin administration led to a change in the patient’s cytokine profile. The UCBCT resulted in an increase in the concentration of HexA in the patient’s serum and in an improvement in the patient’s neurological status. However, neither UCBCT nor curcumin were able to alter HexA activity and the level of GM2 in patient’s plasma. The data obtained indicate that UCBCT and curcumin administration can alter the immunity of a patient with TSD, reduce the level of inflammatory cytokines and thereby improve the patient’s condition.

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Analysis of the Serum Cytokine Profile in Allergic Patients Opens a Way to Personalized Treatment of Allergy

Bulletin of Experimental Biology and Medicine ◽

10.1007/s10517-019-04437-9 ◽

2019 ◽

Vol 166 (6) ◽

pp. 770-773

Author(s):

I. V. Bogdanov ◽

E. I. Finkina ◽

D. N. Melnikova ◽

A. A. Tagaev ◽

Т. V. Ovchinnikova

Keyword(s):

Cytokine Profile ◽

Personalized Treatment ◽

Serum Cytokine

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Serum cytokine profile during subcutaneous implantation of the decellularized esophagus matrix in rats

Journal of Biomaterials Applications ◽

10.1177/0885328220935037 ◽

2020 ◽

Vol 35 (3) ◽

pp. 446-455

Author(s):

Karina Melkonyan ◽

Ramazan Nakokhov ◽

Tatyana Rusinova ◽

Yana Yutskevich ◽

Ilya Bykov ◽

...

Keyword(s):

Dynamic Change ◽

Healing Process ◽

Cytokine Profile ◽

Control Group ◽

Wound Healing Process ◽

Subcutaneous Implantation ◽

Serum Cytokine ◽

Serum Samples ◽

Rat Serum ◽

Gm Csf

Aim to study non-specific immune response characteristics (serum cytokine profile) in rats after subcutaneous implantation of the decellularized esophagus matrix. Methods Data were obtained in Wistar rats. The rats underwent subcutaneous implantation of decellularized esophagus (DE) and native allogeneic esophagus (NE). Explantation of sampling were carried out on the 7th, 14th and 21st day of the experiment. Explanted NEs and DEs were processed for histologic examination. The content of IL1α, IL2, IL4, IL17А, TNFα, IFNγ and GM-CSF in serum samples were tested by ELISA. Results In rat serum with DEs on the 7th day of the experiment it was significant increase in IL1α level in comparison with control group, IL2, TNFα, IL4 levels did not differ from the control group levels that indicates the stabilization of inflammation. The content of IL17A, IFNγ and GM-CSF significantly decreased compared to control. On the 14th day, IL17A concentration analysis showed a sharp decrease in comparison with the the 7th experimental day. We found decrease in IL1α level vs control group and decrease in IFNγ level vs 7th day. On the 21st experimental day was shown a significant decrease in the IL17A, IFNγ and IL1α content in DE rats. Conclusions It was found dynamic change in studied rat cytokine concentrations that correspond to favourable clinical picture in DE group in comparison with an active inflammatory reaction in NE group. IL1α, IL4, IL17A and IFNγ concentrations reflect positive dynamics of the wound healing process and the absence of local inflammation and rejection of decellularized matrices.

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