Detection of Anaplasma phagocytophilum in European brown hares ( Lepus europaeus ) using three different methods

2021 ◽  
Author(s):  
Paulina Maria Lesiczka ◽  
David Modry ◽  
Hein Sprong ◽  
Manoj Fonville ◽  
Jiri Pikula ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Lepus Europaeus

scholarly journals Serological Evidence of Natural Exposure to Tick-Borne Pathogens in Horses, Romania

2021 ◽  
Vol 9 (2) ◽  
pp. 373
Author(s):  
Andreea Monica Bogdan ◽  
Mariana Ionita ◽  
Ioan Liviu Mitrea
Keyword(s):  
Confidence Interval ◽  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Serological Evidence ◽  
North Central ◽  
Natural Exposure

The purpose of this study was to investigate the seroprevalence of selected tick-borne-pathogens (TBPs) among Romanian horses. For this, a total of 223 animals originating from north, central, and southeast Romania, including horses from stud farms (n = 118) and working horses (n = 105), were tested using a commercial rapid ELISA-based test. Overall, 10.3% (95% confidence interval (CI): 6.7–15.1%) of the tested horses were seropositive for antibodies (Ab) against Anaplasma phagocytophilum. Additionally, 18.8% (95% CI: 13.9–24.6%) and 0.5% (95% CI: 0.01–2.5%) of horses were seropositive for Ab against Borrelia burgdorferi sensu lato and Ehrlichia spp., respectively. Among the tested horses, 3.1% were seroreactive to two or three pathogens. These findings show the natural exposure of Romanian horses to zoonotic tick-borne pathogens and emphasize the need for further studies to better understand the epidemiology of equine tick-borne diseases in Romania.

scholarly journals Granulocytic anaplasmosis in captive ring-tailed lemur (Lemur catta) in Poland

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Łukasz Adaszek ◽  
Anna Wilczyńska ◽  
Jerzy Ziętek ◽  
Marcin Kalinowski ◽  
Oliwier Teodorowski ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Polymerase Chain Reaction Test ◽  
Anaplasma Ovis ◽  
Case Presentation ◽  
Granulocytic Anaplasmosis ◽  
Pcr Product ◽  
Polymerase Chain ◽  
Obligate Intracellular Bacteria ◽  
Chain Reaction Test ◽  
Anaplasma Bovis

Abstract Background Anaplasma are obligate intracellular bacteria and aetiological agents of tick-borne diseases of both veterinary and medical interest. The genus Anaplasma comprises six species: Anaplasma marginale, Anaplasma centrale, Anaplasma ovis, Anaplasma phagocytophilum, Anaplasma bovis and Anaplasma platys. They can infect humans, carnivores, ruminants, rodents, insectivores, birds and reptiles. The aim of this study was to present the first clinical case of granulocytic anaplasmosis in a captive ring-tailed lemur in Poland. Case presentation A 4-year-old female lemur presented anorexia, epistaxis and tick infestation. The microscopic examination of a blood smear revealed morulae in neutrophils. Polymerase chain reaction test and sequencing of obtained PCR product confirmed infection by the GU183908 Anaplasma phagocytophilum strain. Therapeutic protocol included doxycycline (2.5 mg/kg p.o., b.i.d.) for 3 weeks and the lemur recovered within 24 h. Conclusions This is the first report on granulocytic anaplasmosis in a ring-tailed lemur in Europe, indicating that A. phagocytophilum infection must also be considered in differential diagnosis in this animal species, especially in individuals with thrombocytopenia associated with Ixodes ricinus parasitism.

scholarly journals Novel Protozoans in Austria Revealed through the Use of Dogs as Sentinels for Ticks and Tick-Borne Pathogens

2021 ◽  
Vol 9 (7) ◽  
pp. 1392
Author(s):  
Michiel Wijnveld ◽  
Anna-Margarita Schötta ◽  
Theresa Stelzer ◽  
Georg Duscher ◽  
Michael Leschnik ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Blot Hybridization ◽  
Dermacentor Reticulatus ◽  
Sensu Stricto ◽  
Babesia Microti ◽  
Rickettsia Helvetica ◽  
Haemaphysalis Concinna ◽  
Borrelia Lusitaniae ◽  
Rickettsia Monacensis ◽  
Borrelia Burgdorferi Sensu Stricto

We previously isolated and cultivated the novel Rickettsia raoultii strain Jongejan. This prompted us to ask whether this strain is unique or more widely present in Austria. To assess this issue, we retrospectively screened ticks collected from dogs in 2008. Of these collected ticks, we randomly selected 75 (47 females and 28 males) Dermacentor reticulatus, 44 (21 females, 7 males, and 16 nymphs) Haemaphysalis concinna, and 55 (52 females and 3 males) ticks of the Ixodes ricinus complex. Subsequently, these ticks were individually screened for the presence of tick-borne pathogens using the reverse line blot hybridization assay. In our current study, we detected DNA from the following microbes in D. reticulatus: Anaplasma phagocytophilum, Borrelia lusitaniae, Borrelia spielmanii, Borrelia valaisiana, and R. raoultii, all of which were R. raoultii strain Jongejan. In H. concinna, we found DNA of a Babesia sp., Rickettsia helvetica, and an organism closely related to Theileria capreoli. Lastly, I. ricinus was positive for Anaplasma phagocytophilum, Borrelia afzelii, Borrelia burgdorferi sensu stricto, Borrelia garinii/Borrelia bavariensis, B. lusitaniae, B. spielmanii, B. valaisiana, Candidatus Neoehrlichia mikurensis, Rickettsia helvetica, Rickettsia monacensis, and Theileria (Babesia) microti DNA. The detection of DNA of the Babesia sp. and an organism closely related to Theileria capreoli, both found in H. concinna ticks, is novel for Austria.

scholarly journals A preliminary study on the detection of potential contaminants in the European brown hare (Lepus europaeus) by suspect and microplastics screening

2021 ◽  
Vol 4 ◽  
pp. 100045
Author(s):  
Romana Hornek-Gausterer ◽  
Herbert Oberacher ◽  
Vera Reinstadler ◽  
Christina Hartmann ◽  
Bettina Liebmann ◽  
...  
Keyword(s):  
Lepus Europaeus ◽  
Brown Hare ◽  
European Brown Hare ◽  
Preliminary Study

A new genetic approach to distinguish strains of Anaplasma phagocytophilum that appear not to cause human disease

2021 ◽  
Vol 12 (3) ◽  
pp. 101659
Author(s):  
Dionysios Liveris ◽  
Maria E. Aguero-Rosenfeld ◽  
Thomas J. Daniels ◽  
Sandor Karpathy ◽  
Christopher Paddock ◽  
...  
Keyword(s):  
Human Disease ◽  
Anaplasma Phagocytophilum ◽  
Genetic Approach

scholarly journals An efficient microinjection method to generate human anaplasmosis agent Anaplasma phagocytophilum-infected ticks

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Vikas Taank ◽  
Ellango Ramasamy ◽  
Hameeda Sultana ◽  
Girish Neelakanta
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Ixodes Scapularis ◽  
In Vitro Cultures ◽  
Transmission Dynamics ◽  
Tick Feeding ◽  
Nymphal Stage ◽  
Bacterial Colony ◽  
Obligate Intracellular ◽  
Transstadial Transmission

Abstract Ticks are important vectors that transmit several pathogens including human anaplasmosis agent, Anaplasma phagocytophilum. This bacterium is an obligate intracellular rickettsial pathogen. An infected reservoir animal host is often required for maintenance of this bacterial colony and as a source for blood to perform needle inoculations in naïve animals for tick feeding studies. In this study, we report an efficient microinjection method to generate A. phagocytophilum-infected ticks in laboratory conditions. The dense-core (DC) form of A. phagocytophilum was isolated from in vitro cultures and injected into the anal pore of unfed uninfected Ixodes scapularis nymphal ticks. These ticks successfully transmitted A. phagocytophilum to the murine host. The bacterial loads were detected in murine blood, spleen, and liver tissues. In addition, larval ticks successfully acquired A. phagocytophilum from mice that were previously infected by feeding with DC-microinjected nymphal ticks. Transstadial transmission of A. phagocytophilum from larvae to nymphal stage was also evident in these ticks. Taken together, our study provides a timely, rapid, and an efficient method not only to generate A. phagocytophilum-infected ticks but also provides a tool to understand acquisition and transmission dynamics of this bacterium and perhaps other rickettsial pathogens from medically important vectors.

Sign in / Sign up

Export Citation Format

Share Document