scholarly journals Granulocytic anaplasmosis in captive ring-tailed lemur (Lemur catta) in Poland

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Łukasz Adaszek ◽  
Anna Wilczyńska ◽  
Jerzy Ziętek ◽  
Marcin Kalinowski ◽  
Oliwier Teodorowski ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Polymerase Chain Reaction Test ◽  
Anaplasma Ovis ◽  
Case Presentation ◽  
Granulocytic Anaplasmosis ◽  
Pcr Product ◽  
Polymerase Chain ◽  
Obligate Intracellular Bacteria ◽  
Chain Reaction Test ◽  
Anaplasma Bovis

Abstract Background Anaplasma are obligate intracellular bacteria and aetiological agents of tick-borne diseases of both veterinary and medical interest. The genus Anaplasma comprises six species: Anaplasma marginale, Anaplasma centrale, Anaplasma ovis, Anaplasma phagocytophilum, Anaplasma bovis and Anaplasma platys. They can infect humans, carnivores, ruminants, rodents, insectivores, birds and reptiles. The aim of this study was to present the first clinical case of granulocytic anaplasmosis in a captive ring-tailed lemur in Poland. Case presentation A 4-year-old female lemur presented anorexia, epistaxis and tick infestation. The microscopic examination of a blood smear revealed morulae in neutrophils. Polymerase chain reaction test and sequencing of obtained PCR product confirmed infection by the GU183908 Anaplasma phagocytophilum strain. Therapeutic protocol included doxycycline (2.5 mg/kg p.o., b.i.d.) for 3 weeks and the lemur recovered within 24 h. Conclusions This is the first report on granulocytic anaplasmosis in a ring-tailed lemur in Europe, indicating that A. phagocytophilum infection must also be considered in differential diagnosis in this animal species, especially in individuals with thrombocytopenia associated with Ixodes ricinus parasitism.

scholarly journals Computerized tomography scan findings of a patient with severe enterocolitis associated with the coronavirus disease 2019: a case report

2020 ◽  
Author(s):  
Katsunari Takifuji ◽  
Hiroshi Terasawa ◽  
Masami Oka ◽  
Masaki Sahara ◽  
Tomoko Hara ◽  
...  
Keyword(s):  
Ct Scan ◽  
Imaging Modality ◽  
Lower Abdominal Pain ◽  
Polymerase Chain Reaction Test ◽  
Bloody Stool ◽  
Case Presentation ◽  
Reactive Protein ◽  
First Case ◽  
Polymerase Chain ◽  
Chain Reaction Test

Abstract Background: Currently, there is no available study about the occurrence of enterocolitis associated with the coronavirus disease 2019 (COVID-19) among adults. Case presentation: A 56-year-old man had lower abdominal pain, distension, diarrhea, and bloody stool. Moreover, the patient presented with fever and general fatigue. Laboratory examination did not reveal leukocytosis nor neutrophilia. However, the patient’s C-reactive protein level increased to 4.9 (normal range: 0.0–0.3) mg/dL. Both sputum and stool cultures had normal results. However, abdominal computed tomography (CT) scan revealed wall thickness mainly in the submucosal layer of the left part of the large intestine. Thus, real-time polymerase chain reaction test of throat swabs was performed for the diagnosis of COVID-19, and positive results were obtained. Conclusions: We report the first case of enterocolitis correlated to COVID-19, and the patient had positive abdominal CT scan findings. This imaging modality could be effective for the diagnosis of enterocolitis associated with COVID-19.

scholarly journals First identification of Anaplasma phagocytophilum in both a biting tick Ixodes nipponensis and a patient in Korea: a case report

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Seung Hun Lee ◽  
Na-Ri Shin ◽  
Choon-Mee Kim ◽  
Sungdo Park ◽  
Na Ra Yun ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Leukemia Cell ◽  
Tick Bite ◽  
Detection Methods ◽  
Leukemia Cell Line ◽  
Rrna Gene ◽  
Nested Polymerase Chain Reaction ◽  
Case Presentation ◽  
Granulocytic Anaplasmosis ◽  
Polymerase Chain

Abstract Background Human granulocytic anaplasmosis (HGA) is a tick-borne infectious disease caused by Anaplasma phagocytophilum. To date, there have been no reported cases of A. phagocytophilum infection found in both the biting tick and the patient following a tick bite. Case presentation An 81-year-old woman presented with fever following a tick bite, with the tick still intact on her body. The patient was diagnosed with HGA. The tick was identified as Ixodes nipponensis by morphological and molecular biological detection methods targeting the 16S rRNA gene. The patient’s blood was cultured after inoculation into the human promyelocytic leukemia cell line HL-60. A. phagocytophilum growth was confirmed via culture and isolation. A. phagocytophilum was identified in both the tick and the patient’s blood by Anaplasma-specific groEL- and ankA-based nested polymerase chain reaction followed by sequencing. Moreover, a four-fold elevation in antibodies was observed in the patient’s blood. Conclusion We report a case of a patient diagnosed with HGA following admission for fever due to a tick bite. A. phagocytophilum was identified in both the tick and the patient, and A. phagocytophilum was successfully cultured. The present study suggests the need to investigate the possible incrimination of I. nipponensis as a vector for HGA in Korea.

scholarly journals Molecular survey of zoonotic Anaplasma phagocytophilum and genetic evidence of a putative novel Anaplasma species in goats from Taif, Saudi Arabia

2019 ◽  
pp. 54-59
Author(s):  
Mohamed W. Ghafar ◽  
Sayed A. M. Amer
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Public Health Importance ◽  
Specific Primers ◽  
Anaplasma Ovis ◽  
Variant Strain ◽  
Molecular Identity ◽  
Complete Identification ◽  
Polymerase Chain ◽  
Anaplasma Bovis ◽  
Positive Results

Aim: Genus Anaplasma is of veterinary and public health importance, and its members utilize ruminants as key hosts in their epidemiology. To date, information about the occurrence and molecular identity of Anaplasma phagocytophilum and other Anaplasma species in Saudi Arabian goats is scarce. This study aimed to molecularly detect and characterize zoonotic A. phagocytophilum and other Anaplasma spp. in goats from Taif District, KSA. Materials and Methods: Blood samples collected from 67 goats were polymerase chain reaction tested using common and A. phagocytophilum-specific primers targeting 16S rRNA and msp4 genes, respectively. Amplicons of common reactions were purified, sequenced, and analyzed. Results: Six goats yielded positive results with common primers, whereas all animals proved negative for A. phagocytophilum. Analysis of the two successfully sequenced amplicons revealed the presence of a variant strain of Anaplasma ovis (99.52% ID) and a new Anaplasma organism, which was clustered with Anaplasma bovis (95.9% ID) and Aegyptianella pullorum (94.99% ID) and distinctly separated from all other recognized species of the genus Anaplasma. Conclusion: The tested goats proved negative for A. phagocytophilum; however, we could not confirm that the area is pathogen free. A variant strain of A. ovis and a putative novel Anaplasma spp. were reported raising the concern of veterinary and zoonotic potential. Other genes should be sequenced and analyzed for complete identification of the detected organisms.

scholarly journals Clinical Evaluation of a 16S Ribosomal RNA Polymerase Chain Reaction Test for the Diagnosis of Lymph Node Tuberculosis

2006 ◽  
Vol 43 (7) ◽  
pp. 855-859 ◽  
Author(s):  
Fernando Osores ◽  
Oscar Nolasco ◽  
Kristien Verdonck ◽  
Jorge Arevalo ◽  
Juan Carlos Ferrufino ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Lymph Node ◽  
Rna Polymerase ◽  
Ribosomal Rna ◽  
Polymerase Chain Reaction Test ◽  
Chain Reaction ◽  
Reaction Test ◽  
Polymerase Chain ◽  
Lymph Node Tuberculosis ◽  
Chain Reaction Test

Polymerase Chain Reaction for the Direct Detection of Brucella spp. in Milk and Cheese

2001 ◽  
Vol 64 (2) ◽  
pp. 164-167 ◽  
Author(s):  
G. TANTILLO ◽  
A. DI PINTO ◽  
A. VERGARA ◽  
C. BUONAVOGLIA
Keyword(s):  
Polymerase Chain Reaction ◽  
Direct Detection ◽  
Polymerase Chain Reaction Test ◽  
Chain Reaction ◽  
Sheep Milk ◽  
Reaction Test ◽  
Polymerase Chain ◽  
Quality Of Milk ◽  
Chain Reaction Test

A polymerase chain reaction test was developed to detect Brucella spp. directly in milk and cheese and optimized using primers for the BSCP-31 gene. A total of 46 cheese samples produced with sheep and goats milk were assayed, and Brucella spp. was detected in 46% of them, especially in cheese made from sheep milk. This method is of remarkable epidemiologic interest because it is an indirect test indicating the sanitary quality of milk used in dairy industries. The method showed good sensitivity and specificity. It is faster and less expensive than the conventional bacteriological assays.

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